Antibacterial and phytochemical properties of Himachal white-rot fungi.
Journal name: World Journal of Pharmaceutical Research
Original article title: Study of antibacterial and phytochemical properties of white-rot fungi collected from the northwestern regions of himachal pradesh
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Jyoti Sharma, Astha Tripathi, Pradeep Kumar, Isha Sharma, Monika Thakur, Neha Thakur and Shaveta Singh
World Journal of Pharmaceutical Research:
(An ISO 9001:2015 Certified International Journal)
Full text available for: Study of antibacterial and phytochemical properties of white-rot fungi collected from the northwestern regions of himachal pradesh
Source type: An International Peer Reviewed Journal for Pharmaceutical and Medical and Scientific Research
Doi: 10.20959/wjpr201716-10134
Copyright (license): WJPR: All rights reserved
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Summary of article contents:
Introduction
Mushrooms have gained recognition as functional foods and sources of various medicinal compounds and nutraceuticals. This study investigates the antibacterial and phytochemical properties of six mushroom cultures collected from the northwestern regions of Himachal Pradesh. The research evaluates the methanol and hot water extracts of these mushrooms, specifically targeting their efficacy against several pathogenic bacteria, including Staphylococcus aureus, Pseudomonas aeruginosa, Escherichia coli, Salmonella typhi, and Klebsiella pneumoniae. The findings suggest the potential of these mushrooms as sources of bioactive compounds with significant antibacterial activity.
Antibacterial Activity of Mushroom Extracts
The study demonstrated that methanol and hot water extracts from various mushroom cultures showed significant antimicrobial activity. Specifically, the methanolic extract of Agaricus sp. exhibited the highest antibacterial activity against K. pneumoniae with a zone of inhibition of 36±1.4 mm, while the hot water extract of Chlorophyllum sp. showed maximum activity against the same bacterium at 37±1.4 mm. In terms of minimal inhibitory concentration (MIC), the extracts generally demonstrated good efficacy, with some showing MIC values as low as 0.78 mg/ml. This highlights the potential of these mushrooms in the development of novel antibacterial agents.
Phytochemical Analysis of Extracts
A comprehensive phytochemical analysis revealed the presence of various bioactive compounds in the mushroom extracts, including alkaloids, carbohydrates, flavonoids, phenolic compounds, terpenoids, and tannins. Notably, the hot water extract of Chlorophyllum sp. was found to have the highest phenolic content at 7.77±0.9 mg/g, while Schizophyllum sp. contained the most flavonoids at 17.94±13.1 mg/g. This information underscores the nutritional richness and medicinal properties of these mushrooms, as flavonoids and phenolic compounds are known for their health benefits, including antioxidant effects and potential antibacterial activity.
Role of Mushrooms in Medicine and Nutrition
Beyond their antibacterial properties, mushrooms are recognized for their nutritional content, being rich in essential amino acids and other beneficial compounds. This study supports the historical use of mushrooms in traditional medicine and food, indicating that these organisms could be key in combating health issues related to human nutrition and medicine due to their bioactive properties. The presence of antioxidant compounds in mushrooms emphasizes their potential role in promoting health and preventing diseases influenced by oxidative stress.
Conclusion
The research concludes that the examined mushroom cultures from Himachal Pradesh present a promising source of bioactive compounds with both antibacterial and antioxidant properties. The diverse phytochemical profile supports the traditional use of mushrooms in medicine and suggests potential applications in food safety and nutrition. Further studies are encouraged to explore the industrial cultivation of these mushrooms, aiming to enhance the production of their bioactive metabolites and exploit their health benefits more thoroughly. Overall, the findings highlight the significant role mushrooms can play in both culinary and medicinal fields.
FAQ section (important questions/answers):
What types of mushrooms were tested for antibacterial activity?
The study tested six mushroom cultures: Morchella sp., Mycena sp., Trametes sp., Agaricus sp., Chlorophyllum sp., and Schizophyllum sp. Each was evaluated for its potential antibacterial properties against specific pathogenic bacteria.
Which mushroom showed the highest antibacterial activity?
The methanol extract of Agaricus sp. exhibited the highest antibacterial activity, with a zone of inhibition of 36±1.4 mm against Klebsiella pneumoniae, followed closely by Chlorophyllum sp.
What methods were used to extract compounds from mushrooms?
Mushroom extracts were prepared using two methods: a methanolic extraction and a hot water extraction. Both methods aimed to isolate bioactive compounds for further testing.
What are the key phytochemical compounds found in the extracts?
The extracts contained various phytochemicals, including alkaloids, tannins, phenols, flavonoids, and terpenoids. The presence of these compounds suggests potential health benefits and bioactive properties.
What pathogenic bacteria were investigated in the study?
Five pathogenic bacteria were investigated: Klebsiella pneumoniae, Salmonella typhi, Staphylococcus aureus, Escherichia coli, and Pseudomonas aeruginosa, which are significant in medical and clinical settings.
Can the mushrooms be used for medicinal purposes?
Yes, the mushrooms tested showed promising antibacterial and antioxidant activities, indicating their potential for use in developing new medicines and nutraceuticals, supporting traditional medicinal practices.
Glossary definitions and references:
Scientific and Ayurvedic Glossary list for “Antibacterial and phytochemical properties of Himachal white-rot fungi.�. This list explains important keywords that occur in this article and links it to the glossary for a better understanding of that concept in the context of Ayurveda and other topics.
1) Activity:
In the context of the study, 'activity' refers to the effectiveness of the mushroom extracts in inhibiting the growth of pathogenic bacteria. This microbial activity is a key focus of research in pharmacology and nutrition, providing insights into potential therapeutic applications of mushrooms as natural antimicrobials.
2) Water:
The term 'water' is important in the study as it relates to the preparation of hot water extracts from mushroom cultures. Water extracts are utilized to assess the solubility of various phytochemicals and their biological activities, offering insights into the therapeutic potential and natural properties of mushrooms.
3) Antibiotic (Antibacterial):
The term 'anti-bacterial' describes the property of preventing the growth of bacteria. This study focuses on this attribute in mushroom extracts, emphasizing their potential role as natural remedies in combating bacterial infections amid increasing antibiotic resistance.
4) Flavonoid:
Flavonoids are a class of phytonutrients found in many plants and fungi, noted for their antioxidant and anti-inflammatory properties. In this study, the presence of flavonoids in mushroom extracts underscores their potential health benefits, including antimicrobial effects that could play a role in preventing infections.
5) Phytochemical:
Phytochemicals are naturally occurring chemical compounds in plants known for their health-promoting properties. In this research, identifying phytochemicals in mushrooms helps establish their medicinal value, promoting their use in diets and traditional medicine as sources of antioxidants and antimicrobial agents.
6) Aureus:
'Aureus' specifically refers to Staphylococcus aureus, a common pathogenic bacterium that the study's mushroom extracts were tested against. Understanding the inhibitory effects on this bacterium is vital since it is often associated with various infections, reflecting the practical importance of the research.
7) Table:
In scientific studies, a 'table' is a structured arrangement of data that summarizes findings succinctly. In this research, tables are used to present the antibacterial activity results, facilitating comparisons across different mushroom extracts, thus enhancing the communication of important quantitative findings.
8) Study (Studying):
'Study' refers to the systematic investigation undertaken to explore the antibacterial properties and phytochemical composition of mushrooms. The rigor of the study is crucial for validating results, ensuring findings are reliable, and contributing significantly to the field of pharmacological research.
9) Species:
The term 'species' denotes the different types of mushrooms analyzed in the research. Identifying specific species is significant as each may exhibit unique properties and efficacy against pathogens, influencing their application in alternative medicine and antibiotic development.
10) Food:
'Food' relates to the nutritional aspect of mushrooms, indicating their potential role as functional foods rich in bioactive compounds. The study emphasizes the dual benefit of mushrooms as both food sources and therapeutic agents, aligning with contemporary health and wellness trends.
11) Pharmacological:
'Pharmacological' pertains to the study of drugs and their effects. In this context, it highlights the potential medicinal uses of mushroom-derived compounds, suggesting avenues for future drug development aimed at treating infections and improving health outcomes.
12) Medicine:
The term 'medicine' encompasses the sciences of diagnosing, treating, and preventing illness. This study adds to the body of medical research that investigates natural compounds, positioning mushrooms as viable sources for new therapeutic agents due to their antimicrobial properties.
13) Cancer:
Cancer refers to a group of diseases characterized by uncontrolled cell growth. The study indirectly addresses cancer through the investigation of phenolic compounds and antioxidants in mushrooms, suggesting potential protective properties against oxidative stress and inflammation, relevant in cancer research.
14) Medium:
'Medium' typically refers to the substrate on which bacteria or fungi grow. In this research, it signifies the nutrient media used for cultivating pathogenic bacteria and mushrooms, indicating the conditions under which various tests, including antibacterial activity, were conducted.
15) Substance:
The term 'substance' describes the specific extracts obtained from mushrooms. Identifying these beneficial substances is crucial in understanding their biological activities, guiding further research into their therapeutic applications and the mechanisms of action against various pathogens.
16) Quality:
'Quality' in this study refers to the effectiveness and properties of mushroom extracts, emphasizing the importance of high-quality collection and preparation methods to ensure that the active compounds retain efficacy for antibacterial activity and potential therapeutic use.
17) Disease:
The term 'disease' reflects the pathogenic conditions targeted by the study's findings, particularly bacterial infections. Identifying natural remedies for diseases caused by bacteria is central to addressing public health challenges in an era of increasing antibiotic resistance.
18) Science (Scientific):
'Science' encompasses the systematic enterprise that builds and organizes knowledge in the form of testable explanations and predictions about the universe. This study exemplifies scientific research aimed at understanding natural products and their application in health and medicine.
19) Sharman (Sarma, Sharma, Sarman):
Sharma refers to one of the authors of the study, emphasizing contributions from the academic community in exploring the antibacterial properties of mushrooms. Acknowledging the researchers enhances credibility and highlights the collaborative nature of scientific exploration.
20) Colar (Colan):
'Solan' indicates the geographical location where the study was conducted, specifically the Mycology Research Laboratory at Shoolini University, highlighting the importance of regional biodiversity and local resources in scientific research on mushrooms.
21) Rich (Rch):
The term 'rich' describes the abundance of bioactive compounds present in mushrooms. It emphasizes the nutritional and medicinal value of mushrooms, suggesting their potential to contribute positively to human health as sources of antioxidants and natural drugs.
22) Wall:
'Wall' relates to the structure of the fungal cells, particularly the cell wall components that may possess immunomodulatory properties. Understanding these structures is critical in assessing the biological activities of mushrooms and their potential therapeutic applications.
23) Discussion:
'Discussion' refers to a section in research that interprets the findings, compares them with previous studies, and contextualizes the results. This element is crucial for drawing conclusions and suggesting implications for future research and applications in medicine.
24) Richness:
'Richness' denotes the variety and abundance of phytochemicals present in mushrooms. The study highlights this aspect to emphasize the potential diversity of compounds that could be harnessed for various health benefits, particularly in drug discovery.
25) Surface:
'Surface' refers to the external area of the agar plates used in experiments, where mushroom extracts were applied. This area plays a critical role in observing antimicrobial effects as it serves as the interface for interaction between the extracts and bacterial growth.
26) Family:
'Family' in a biological context refers to a group of related organisms. In this study, recognizing the family of mushrooms aids in understanding their evolutionary relationships, biodiversity, and the potential for discovering new species with medicinal properties.
27) Reason:
'Reason' pertains to the rationale behind conducting the study. This includes the need for alternative antimicrobial agents, the rich diversity of mushrooms, and their potential health benefits, thus justifying the investigation of their bioactive compounds.
28) Animal:
'Animal' refers to the biological classification of multicellular organisms. The study draws comparisons indicating that some compounds found in mushrooms may offer equivalent nutritional benefits to animal products, promoting mushrooms as sources of essential nutrients for human health.
29) Powder:
'Powder' refers to the form in which mushroom extracts may be prepared for experiments. This aspect highlights a method of processing mushrooms to facilitate the extraction of bioactive compounds, essential for conducting various assays in the study.
30) Kumar:
'Kumar' indicates one of the authors of the study, showcasing the collaborative efforts in research. Acknowledging the contribution of various researchers enriches the academic dialogue and emphasizes the importance of teamwork in scientific inquiry.
31) Isha (Isa):
'Isha' refers to an author involved in the research, highlighting the diverse contributions to the study. Acknowledging all researchers is crucial in recognizing their collective efforts towards understanding the medicinal properties of mushrooms.
32) Meat:
'Meat' signifies a primary source of protein in many diets. By comparing mushrooms to meat, the study emphasizes the nutritional value of mushrooms as plant-based sources and their potential role in addressing protein needs amidst dietary shifts.
33) Milk:
'Milk' refers to a fundamental source of nutrition, particularly in many cultures. The comparison with mushrooms underscores mushrooms' potential as alternative sources of essential nutrients, supporting plant-based diets and enhancing options for health-conscious individuals.
34) Drug:
'Drug' refers to substances used for medical treatment. The potential of mushroom extracts as natural drugs is a primary focus of the study, highlighting their antibacterial properties and roles in developing new treatments against bacterial infections.
35) Soil:
'Soil' refers to the natural environment where mushrooms grow, emphasizing the ecological aspect of fungiculture. The study's findings may enhance the understanding of how soil quality and composition impact the growth and medicinal properties of mushrooms.
Other Science Concepts:
Discover the significance of concepts within the article: �Antibacterial and phytochemical properties of Himachal white-rot fungi.�. Further sources in the context of Science might help you critically compare this page with similair documents:
Flavonoid, Traditional medicine, Antibacterial activity, Pharmacological activities, Antioxidant properties, Minimum inhibitory concentration, Pathogenic bacteria, Quantitative analysis, Secondary metabolite, Methanolic extract, Nutraceutical, Bioactive compound, Hot water extract, Functional food, Saponin, Terpenoid, Antimicrobial compound, Phyto-chemical properties, Phenolic compound, Active component, Antioxidant function, Glycoside.