Significance of Reverse transcriptase PCR
Reverse transcriptase PCR is a molecular biology technique that enables the detection and quantification of specific gene expressions, including genes like beta-actin and osteocalcin, in differentiated cells. This method is widely utilized in research to analyze the expression levels of genes, providing vital insights into cellular functions and processes. By allowing researchers to measure RNA levels, reverse transcriptase PCR serves as a crucial tool in understanding gene regulation and the effects of various biological conditions.
Synonyms: Rt-pcr, Reverse transcription polymerase chain reaction, Reverse transcriptase polymerase chain reaction, Quantitative rt-pcr, Real-time rt-pcr, Reverse transcription pcr, Real-time pcr, Quantitative pcr, Cdna synthesis, Gene expression analysis
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The concept of Reverse transcriptase PCR in scientific sources
Reverse transcriptase PCR is a molecular biology technique utilized to detect and quantify gene expression, including genes like beta-actin and osteocalcin, in differentiated cells, helping to analyze cellular functions and conditions.
From: The Malaysian Journal of Medical Sciences
(1) A technique used to amplify and quantify RNA levels to assess the expression of specific candidate genes.[1]