Significance of Reverse transcription-PCR
Reverse transcription-PCR is a laboratory technique that amplifies RNA by first converting it into complementary DNA (cDNA). This method is essential for generic detection of coronaviruses and allows for differentiation at the prototype strain level. It facilitates the conversion of RNA into cDNA, enabling specific cDNA targets to be amplified for further analysis, making it a crucial tool in molecular biology and virology research.
Synonyms: Rt-pcr, Reverse transcriptase pcr, Quantitative pcr, Real-time pcr
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The concept of Reverse transcription-PCR in scientific sources
Reverse transcription-PCR is a laboratory technique that converts RNA into complementary DNA (cDNA) and amplifies specific cDNA targets, allowing for detailed analysis of gene expression and other RNA-related research.
From: The Malaysian Journal of Medical Sciences
(1) Reverse transcription-PCR, also known as RT-PCR, was used to examine the following genes: nephrin, E-Cadherin, BMP-7, HGF, TNFα, TLR4, NFκB, and GAPDH.[1] (2) This is a laboratory technique used for the generic detection of coronaviruses, allowing for differentiation at the prototype strain level, as mentioned in the provided text.[2]