Significance of Quantitative PCR
Quantitative PCR, often abbreviated as qPCR, is a laboratory technique that quantifies DNA or RNA levels in various samples, facilitating the analysis of gene expression and viral load. It is particularly valuable for detecting specific genetic sequences, such as the EBV DNA genome encoding the BNRF1 gene and analyzing viral infections like CMV. By allowing detailed measurements of nucleic acids, qPCR enhances sensitivity in pathogen detection, providing critical insights into microbial loads and infection statuses.
Synonyms: Real-time pcr, Quantitative polymerase chain reaction, Quantitative real-time pcr, Real-time quantitative pcr, Rt-qpcr
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The concept of Quantitative PCR in scientific sources
Quantitative PCR (qPCR) is a laboratory technique for measuring DNA or RNA amounts, crucial for gene expression analysis, viral infection measurement, and detecting specific genomes, such as EBV in clinical studies.
From: The Malaysian Journal of Medical Sciences
(1) This is a laboratory technique used to amplify and quantify specific DNA sequences, allowing for the measurement of gene expression levels.[1] (2) A laboratory technique used to quantify gene expression of the Hsp90 gene in the study.[2]