Significance of Real-time PCR
Real-time PCR is a molecular biology technique essential for amplifying and quantifying targeted DNA molecules simultaneously. It is particularly noted for its reliability, speed, and cost-effectiveness in diagnosing various infections, including viral load detection and gene expression analysis. This method allows continuous monitoring of the DNA amplification process through fluorescent signals, enhancing the accuracy of results without requiring post-PCR processing. Its application spans diverse studies, including those on viral infections, gene expression levels, and even bacteria quantification.
Synonyms: Quantitative pcr, Real-time quantitative pcr, Real-time polymerase chain reaction, Rt-pcr
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The concept of Real-time PCR in scientific sources
Real-time PCR is a laboratory technique utilized to analyze cytokine expression profiles in somatic cells extracted from infected milk, allowing for precise and quantitative measurement of cellular responses during infection.
From: The Malaysian Journal of Medical Sciences
(1) This type of PCR can be used to confirm the presence of gene amplification, and is used to validate common aberrant regions revealed by SNPA.[1] (2) This is a PCR technique that measures the amount of DNA amplified in real-time, and the text mentions it as a more sophisticated technique.[2] (3) The text mentions real-time PCR and its use in the analysis of relative gene expression data.[3] (4) This is a highly sensitive advancement that does not require post-PCR processing and allows for real-time visualization of amplicons with an increased dynamic range of detection.[4] (5) This is a diagnostic technique, and it is used for the early detection of Fasciola hepatica in sheep, and it involves the amplification and detection of DNA in real-time.[5]