Significance of Real-time polymerase chain reaction
Real-time polymerase chain reaction (RT-PCR) is a versatile laboratory technique utilized in various studies to amplify and quantify DNA and RNA samples. It facilitates the measurement of gene expression levels, such as in specific RNA molecules or in assessing viral loads, including dengue and SARS-CoV-2. RT-PCR allows for real-time visualization of the amplification process, making it crucial for diagnosing diseases and understanding microbial presence in samples, such as Porphyromonas gingivalis in dental research.
Synonyms: Quantitative pcr, Real-time pcr, Pcr assay, Rt-pcr
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The concept of Real-time polymerase chain reaction in scientific sources
Real-time polymerase chain reaction is a technique employed to quantify dengue viral load and evaluate the effectiveness of plant extracts, enabling researchers to monitor and analyze viral response effectively in real-time.
From: The Malaysian Journal of Medical Sciences
(1) This is a method described as being utilized for high-throughput β-thalassaemia carrier screening using allele-specific Q-primer.[1] (2) A method used to quantify the expression of specific RNA molecules, such as vitamin D receptor mRNA, enabling measurement of gene expression.[2] (3) An advanced molecular technique that amplifies DNA samples, providing accurate, reproducible results and allowing live visualization of the amplification process.[3] (4) A technique used in DNA profiling to amplify DNA segments for analysis, it allows for the monitoring of the amplification process in real-time.[4] (5) Real time polymerase chain reaction (RT-PCR) is a laboratory technique used to amplify and simultaneously quantify a targeted DNA molecule, allowing for the measurement of gene expression levels.[5]