Significance of Quantitative real-time PCR
Quantitative real-time PCR, also known as qRT-PCR, is a laboratory technique utilized for quantifying mRNA levels to measure gene expression in cells. It is particularly useful in medical research, as evidenced by its application in quantifying miRNA expression levels in biopsy samples from oral cancer patients. Additionally, qRT-PCR can assess the quantities of DNA or RNA, often for purposes such as evaluating mitochondrial DNA copy number in various research studies.
Synonyms: Real-time pcr, Quantitative pcr, Quantitative polymerase chain reaction, Qrt-pcr, Rt-qpcr, Rt-pcr
The below excerpts are indicatory and do represent direct quotations or translations. It is your responsibility to fact check each reference.
The concept of Quantitative real-time PCR in scientific sources
Quantitative real-time PCR (qRT-PCR) is a technique used to measure miRNA expression levels in biopsy samples, providing crucial insights for diagnosing and understanding oral cancer in patients.
From: The Malaysian Journal of Medical Sciences
(1) A technique used to confirm the presence of gene amplification, aiding in the validation of findings from other genomic analyses.[1] (2) A laboratory technique used to quantify DNA or RNA levels, often employed to assess mitochondrial DNA copy number in research.[2] (3) Quantitative real-time PCR (qRT-PCR) is a laboratory technique used to quantify mRNA levels, allowing for the measurement of gene expression in cells.[3]