Significance of QRT-PCR
QRT-PCR, or Quantitative reverse transcription polymerase chain reaction, is a real-time PCR technique utilized for detecting and quantifying RNA. This method is significant in various scientific and medical applications, allowing researchers to measure RNA levels accurately and efficiently. By providing real-time data, QRT-PCR enhances the ability to analyze gene expression and monitor RNA changes in different conditions, making it a crucial tool in molecular biology and diagnostics.
Synonyms: Real-time pcr, Quantitative pcr, Rt-pcr, Reverse transcription pcr, Quantitative real-time pcr, Rt-qpcr
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The concept of QRT-PCR in scientific sources
QRT-PCR, or Quantitative Reverse Transcription Polymerase Chain Reaction, is a real-time PCR technique essential for detecting and quantifying RNA levels in samples, enabling researchers to analyze gene expression efficiently.
From: The Malaysian Journal of Medical Sciences
(1) This is a technique used to measure mRNA expression, and it was performed to measure the mRNA expression of survivin, caspase-9, TRAIL, DR4, and DcR1 in T98G cells, and is also known as quantitative reverse transcriptase-polymerase chain reaction.[1] (2) This is a technique used to determine the relative mRNA expressions of certain genes, which is important for studying gene expression profiles.[2]