Significance of Reverse transcription–polymerase chain reaction
Reverse transcription–polymerase chain reaction (RT-PCR) is a crucial diagnostic method for confirming the presence of the novel coronavirus, SARS-CoV-2, associated with COVID-19. This laboratory technique detects the virus's genetic material through samples from nasopharyngeal or oropharyngeal swabs, making it the gold standard in COVID-19 testing. Known for its accuracy and reliability, RT-PCR testing is commonly used in healthcare settings to effectively diagnose infections caused by the virus.
Synonyms: Rt-pcr, Reverse transcription pcr, Quantitative rt-pcr, Real-time rt-pcr, Reverse transcriptase pcr, Real-time pcr, Molecular diagnostic technique
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The concept of Reverse transcription–polymerase chain reaction in scientific sources
Reverse transcription–polymerase chain reaction (RT-PCR) is a laboratory method that detects the genetic material of pathogens, including SARS-CoV-2, enabling the testing for COVID-19's presence in samples.
From: The Malaysian Journal of Medical Sciences
(1) This is a molecular biology technique that uses reverse transcription to convert RNA into DNA, followed by polymerase chain reaction to amplify specific DNA sequences.[1] (2) The reverse transcription polymerase chain reaction (RT-PCR) is considered the gold standard for COVID-19 diagnostics, especially real-time RT-PCR, as stated in the document.[2] (3) A laboratory technique used to amplify and analyze RNA, allowing researchers to study gene expression.[3] (4) A molecular testing method preferred for diagnosing SARS-CoV-2 infections, known for its accuracy and reliability.[4]