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Antioxidant, cytotoxic, and phytochemical analysis of Ficus microphylla

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Journal name: World Journal of Pharmaceutical Research
Original article title: Antioxidant, cytotoxic and phytochemical analysis of medicinal plant ficus microphylla
The WJPR includes peer-reviewed publications such as scientific research papers, reports, review articles, company news, thesis reports and case studies in areas of Biology, Pharmaceutical industries and Chemical technology while incorporating ancient fields of knowledge such combining Ayurveda with scientific data.
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Original source:

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Author:

Barsa Pradhan and Sunita Bhatnagar


World Journal of Pharmaceutical Research:

(An ISO 9001:2015 Certified International Journal)

Full text available for: Antioxidant, cytotoxic and phytochemical analysis of medicinal plant ficus microphylla

Source type: An International Peer Reviewed Journal for Pharmaceutical and Medical and Scientific Research

Doi: 10.20959/wjpr202314-29384

Copyright (license): WJPR: All rights reserved


Download the PDF file of the original publication


Summary of article contents:

Introduction

The study investigates the biological potential of the medicinal plant Ficus microphylla, which belongs to the Moraceae family. The research focuses on solvent leaf extracts from the plant and evaluates their antioxidant, cytotoxic, and phytochemical properties. Utilizing various solvent extraction methods, the study reveals significant findings related to the presence of secondary metabolites and their respective biological activities.

Antioxidant Activity of Ficus microphylla

The antioxidant activity was evaluated using both qualitative and quantitative assays. A qualitative assessment through Thin Layer Chromatography (TLC) demonstrated the presence of antioxidant bands in several solvent extracts, notably with chloroform exhibiting the highest number of bands. Quantitative analysis further confirmed the antioxidant potential, particularly in the methanol extract, which showcased the highest Ferric Reducing Antioxidant Power (FRAP) value at 5.12 mM ascorbic acid equivalents. This indicates that Ficus microphylla could serve as a valuable source of natural antioxidants.

Phytochemical Analysis

The phytochemical analysis revealed a rich variety of secondary metabolites within the leaf extracts of Ficus microphylla. The presence of alkaloids was confirmed through multiple tests, while tannins and saponins were found in varying amounts across the different solvent extracts. Notably, the methanol extract demonstrated a high content of phenolic compounds, which are critical for exhibiting antioxidant activity. These findings emphasize the therapeutic potential of Ficus microphylla due to its diverse phytochemical composition.

Cytotoxic Activity

The cytotoxic potential of the leaf extracts was assessed via a brine shrimp lethality assay, wherein the methanol extract displayed the highest toxicity, achieving an inhibition rate of 87.9% at a concentration of 200 µg/ml. The chloroform extract also showed significant activity with a 76.36% inhibition rate at a lower concentration of 100 µg/ml. These results highlight the effectiveness of Ficus microphylla as a potential agent in cytotoxic applications, illustrating its pharmacological relevance.

Conclusion

In summary, the study underscores the promising antioxidant and cytotoxic properties of Ficus microphylla, particularly in its methanol extract. The presence of significant secondary metabolites establishes its potential for medicinal applications. Further research is warranted to explore the specific active compounds and their mechanisms of action, paving the way for potential therapeutic uses. The findings advocate for the importance of Ficus microphylla in traditional medicine and its value in scientific research for developing novel pharmaceutical agents.

FAQ section (important questions/answers):

What is the medicinal significance of Ficus microphylla?

Ficus microphylla belongs to the Moraceae family and has various biological activities, including antioxidant, cytotoxic, and antimicrobial properties. It is traditionally used for its medicinal potential and has been studied for its health benefits.

What types of leaf extracts were analyzed in this study?

The study explored four types of leaf extracts: hexane, chloroform, ethyl acetate, and methanol. These extracts were analyzed for their antioxidant, cytotoxic, and phytochemical properties.

Which extract showed the highest antioxidant activity?

The methanol extract exhibited the highest antioxidant activity, demonstrated through Ferric Reducing Antioxidant Power (FRAP) assay and DPPH scavenging assays, outperforming the other solvent extracts.

How was the cytotoxic activity evaluated in Ficus microphylla?

Cytotoxic activity was assessed using the Brine Shrimp Lethality Assay. The methanol extract showed the highest cytotoxicity, with an 87.9% mortality rate in brine shrimp at a dosage of 200 μg/ml.

What secondary metabolites were detected in the extracts?

The study identified several secondary metabolites, including tannins, saponins, and alkaloids. Methanol extract was particularly rich in phenolics, which contribute to its biological activity.

What methods were used for antioxidant activity analysis?

The antioxidant activity was evaluated using qualitative methods (TLC-based DPPH assay) and quantitative methods (DPPH free radical scavenging assay and FRAP assay) to gauge the efficacy of the extracts.

Glossary definitions and references:

Scientific and Ayurvedic Glossary list for “Antioxidant, cytotoxic, and phytochemical analysis of Ficus microphylla�. This list explains important keywords that occur in this article and links it to the glossary for a better understanding of that concept in the context of Ayurveda and other topics.

1) Activity:
Activity refers to the biological effects that the leaf extracts of Ficus microphylla exhibit, including antioxidant and cytotoxic actions. These activities demonstrate the potential medicinal properties of the plant, showcasing its ability to protect cells from damage and its potential in combating cancer through cytotoxic analysis.

2) Bhatnagar:
Bhatnagar is the surname of one of the authors, Sunita Bhatnagar, who contributed to this study. The presence of authors� names underscores the human element in research and reinforces accountability and credibility in scientific work, highlighting the expertise and contributions of individuals involved in the project.

3) Phytochemical:
Phytochemicals are bioactive compounds found in plants that often possess health benefits. In this study, phytochemical analysis was conducted to identify secondary metabolites like alkaloids and flavonoids in Ficus microphylla, which are linked to the plant's therapeutic potential and its traditional medicinal use.

4) Water:
Water is the solvent used in various methods of extraction and analysis in the study. The role of water is crucial in preparing solutions and dilutions, and it is an essential component in biological assays and extraction processes, reflecting its universal solvent properties in scientific research.

5) Table:
Table refers to the organized presentation of data within the document, summarizing results and comparison of various extracts. Tables facilitate comprehension by visually displaying pertinent information, aiding in the interpretation of experimental findings and supporting the written content with clear, concise numerical evidence.

6) Family:
The family emphasizes the collective grouping of related species within taxonomy that are evaluated. Understanding this concept in botanical research aids scientists in studying shared characteristics among plants, forming a foundation for exploring their traditional medicinal applications and potential interactions within ecosystems.

7) Bhubaneswar (Bhubaneshvar, Bhubanesvar):
Bhubaneswar is the city in India where the research was conducted at the Regional Plant Resource Centre. Its geographical context is significant as it contributes to the local biodiversity and availability of medicinal plants, and it also reflects the cultural and ecological frame in which the study is situated.

8) Study (Studying):
Study denotes the research process conducted to explore the biomedical properties of Ficus microphylla. This term encapsulates the systematic investigation undertaken, including methodologies and analyses, aimed at discovering the scientific basis for the plant's medicinal applications and enhancing understanding of its pharmacological effects.

9) Flavonoid:
Flavonoid is a specific term denoting individual compounds within the broader class of flavonoids. Each flavonoid can have unique biological activities and health benefits, and their presence in Ficus microphylla may contribute to the overall antioxidant and cytotoxic properties, indicating the plant's medicinal significance.

10) Salina (Shalina):
Salina refers to Artemia salina, a species of brine shrimp used as a model organism in the cytotoxicity assays of this study. The use of Salina is relevant for assessing the lethality of extracts, serving as an indicator for potential anti-cancer properties and biological activity of the plant.

11) Genu:
Genu refers to a specific acceptable anatomical or biological context, possibly representing the genus or lineage of species under study. It reinforces the systematic taxonomy of the plant research context, underscoring the importance of structured classification in botanical studies.

12) Wall:
Wall may refer to the structural aspects of experimental setups in laboratory methodologies or possibly in structural anatomy relevant to plant studies. Its functional dimension is crucial for ensuring the integrity and safety of experiments conducted in the context of the research described.

13) Pharmacological:
Pharmacological relates to the study of the interactions, effectiveness, and mechanisms of drugs. In this paper, it encompasses evaluating the medicinal properties of Ficus microphylla extracts in terms of their antioxidant and cytotoxic activities, which may provide insight into potential therapeutic applications.

14) Kannan:
Kannan is part of the address associated with the Regional Plant Resource Centre in Bhubaneswar, India. It reflects the specific location where the research was conducted, highlighting the institution's role in the study, which is crucial for validation and context in scientific research.

15) Powder:
Powder pertains to the form of the dried leaves after grinding, used in the extraction processes. The reduction of the plant material into powder increases the surface area for solvent interaction, optimizing extraction efficiency and thereby enhancing the yield of biologically active compounds.

16) India:
India is the country where the research was conducted, indicating the biodiversity and rich traditional knowledge of medicinal plants available there. The geographical context of India is significant as it directly relates to the local usage, cultural significance, and potential discovery of novel medicinal resources.

17) Tree:
Tree denotes a single specimen of the larger plant category, emphasizing the importance of specific individual plants, such as Ficus microphylla, in research. Each tree provides unique chemical compounds that can have considerable medicinal implications, highlighting the value of tree species in scientific exploration.

18) Rich (Rch):
Rich characterizes the abundance of specific compounds, such as phytochemicals, found within Ficus microphylla. This term illustrates the plant's potential as a source of various beneficial metabolites, emphasizing the importance of exploring such plants for their potential health benefits and their role in traditional medicine.

19) Salt (Salty):
Salt, specifically in the context of the brine shrimp experiment, indicates the use of saline solutions in the preparation of artificial seawater. The relevance of salt lies in creating an environment conducive for brine shrimp, crucial for assessing the cytotoxic activity of the plant extracts under study.

20) Salt water:
Salt water refers to the saline solution used to hatch the brine shrimp, Artemia salina. The significance of salt water is essential in mimicking the natural aquatic habitat of these organisms, thus providing an appropriate medium for evaluating the cytotoxic effects of the plant extracts.

21) Forest:
Forest refers to the ecosystem in which plant species, including Ficus microphylla, ideally thrive. The ecological context denotes the importance of biodiversity, conservation efforts, and sustainable practices in maintaining healthful environments, which are essential for preserving valuable medicinal plant resources.

22) Ficus benghalensis:
Ficus benghalensis, known as the banyan tree, is a prominent species within the Ficus genus recognized for its historical and cultural significance, as well as its medicinal properties. Its mention highlights comparative analyses with other Ficus species, offering insights into the diversity and applications of these plants.

23) Ficus religiosa:
Ficus religiosa, known as the sacred fig, holds substantial cultural and religious significance in various traditions. Including this species emphasizes the importance of understanding the pharmacological roles and attributes shared among Ficus genus members, reinforcing the unity and diversity within the Moraceae family.

24) Observation:
Observation represents individual instances of data collection during the study, affirming the role of careful monitoring in research. Each observation contributes to a broader understanding of the plant's properties and validates the methodologies employed to reach definitive conclusions within the study.

25) Discussion:
Discussion encapsulates the section in research where findings are interpreted and contextualized. This part is vital as it synthesizes results with existing literature, contributing to a better understanding of the study implications, implications for future research, and potential applications of the findings.

26) Pradhan:
Pradhan is the name of a contributing author to the study alongside Bhatnagar. The presence of authors' names indicates collaborative effort in research, emphasizing the importance of teamwork in scientific endeavors and acknowledging the individual contributions of researchers to the field.

27) Species:
Species indicates the specific classification of organisms within the biological taxonomy. In this study, Ficus microphylla is the primary species being analyzed, thus emphasizing the significance of understanding specific traits and properties of species in botanical research and conservation efforts.

28) Account:
Account in this context refers to the descriptive narrative or overview provided on the methodologies, findings, and implications of the study. It serves to inform readers of the research process, insight into results, and the relevance of the findings, enriching the understanding of the plant's properties.

Other Science Concepts:

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Discover the significance of concepts within the article: �Antioxidant, cytotoxic, and phytochemical analysis of Ficus microphylla�. Further sources in the context of Science might help you critically compare this page with similair documents:

Medicinal plant, Active principle, Antioxidant activity, Phytochemical analysis, Traditional use, Antimicrobial activity, Moisture content, Secondary metabolite, Phenolic content, Cytotoxic activity, Total antioxidant activity, DPPH free radical scavenging assay, Methanol extract, Tannin, FRAP assay, Solvent extraction, Biological potential, Percentage yield, Saponin, Alkaloid, Solvent extraction method, Cardiac glycoside, Neuroprotection.

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