Isolation and characterization of paddy soil actinomycetes for antimicrobial properties
Journal name: World Journal of Pharmaceutical Research
Original article title: Isolation and characterization of actinomycetes from paddy soil and evaluation of their antimicrobial activities
The WJPR includes peer-reviewed publications such as scientific research papers, reports, review articles, company news, thesis reports and case studies in areas of Biology, Pharmaceutical industries and Chemical technology while incorporating ancient fields of knowledge such combining Ayurveda with scientific data.
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B.Sreedevi, M.R Thanusree and J.Pramoda kumari
World Journal of Pharmaceutical Research:
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Full text available for: Isolation and characterization of actinomycetes from paddy soil and evaluation of their antimicrobial activities
Source type: An International Peer Reviewed Journal for Pharmaceutical and Medical and Scientific Research
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Summary of article contents:
Introduction
The urgent need for new antibiotics arises from the growing resistance of microbial pathogens to current treatments. Actinomycetes, a prolific source of natural antibiotics, were isolated from paddy soil in Pudipatla near Tirupati, Andhra Pradesh, India, and examined for their antimicrobial activities against various pathogens.
Isolation and Identification of Actinomycetes
Soil samples were collected, air-dried, and processed for actinomycete isolation using serial dilution and plating on starch casein agar. Ten isolates were obtained, three of which (A1, A2, A3) showed significant antimicrobial activity against E. coli, B. subtilis, and S. aureus. These isolates were characterized both morphologically and biochemically, including tests for indole production, citrate utilization, and catalase activity, among others.
Antimicrobial Screening
Primary screening involved a cross-streak method where actinomycete isolates were tested for antibacterial activity on nutrient agar plates. The most promising isolates, A1, A2, and A3, were further evaluated using disc diffusion and well diffusion assays. The results revealed that A1 and A3 exhibited significant antagonistic activity against both Gram-positive and Gram-negative bacteria, whereas A2 showed selective inhibition against Gram-positive bacteria.
Chemical Analysis and Bioautography
The antimicrobial compounds produced by actinomycetes were partially purified using Thin Layer Chromatography (TLC). The ethyl acetate extracts were analyzed, revealing several bioactive compounds. Bioautography confirmed the presence of antibacterial substances, with inhibition zones observed around the TLC spots, indicating effective antimicrobial activity.
Conclusion
The study successfully isolated and characterized actinomycetes from paddy soil, highlighting their potential in producing novel antibiotics effective against resistant pathogens. The isolates demonstrated significant antimicrobial activity, especially Staphylococcus aureus, showcasing the promise of actinomycetes as a valuable source for new drug development.
FAQ section (important questions/answers):
What is the focus of the research study?
The study focuses on isolating and characterizing Actinomycetes from paddy soil, evaluating their antimicrobial activities, and identifying potential new antibiotic compounds.
What methods were used for screening Actinomycetes?
The modified agar-cross streak and agar-disc well diffusion methods were employed for primary and secondary screening of antimicrobial activities in the isolated Actinomycetes.
Which bacteria were tested for antimicrobial activity?
The antibacterial activity was tested against three strains: Escherichia coli (E. coli), Bacillus subtilis (B. subtilis), and Staphylococcus aureus (S. aureus).
What are the key findings from Thin Layer Chromatography?
TLC showed active substances with Rf values of 4, 6, and 7 for isolates A1, A2, and A3, respectively. These substances exhibited antimicrobial properties.
How was the antibiotic activity detected in TLC?
Antibiotic activity was detected using a bioautography method by overlaying agar seeded with Staphylococcus aureus on the TLC plate. This localized the antimicrobial activity directly on the chromatogram.
What were the results of the bioautography test?
The bioautography test showed inhibition zones around 0.8 cm for all three Actinomycetes isolates (A1, A2, A3) against Staphylococcus aureus, indicating effective antimicrobial activity.
Glossary definitions and references:
Scientific and Ayurvedic Glossary list for “Isolation and characterization of paddy soil actinomycetes for antimicrobial properties�. This list explains important keywords that occur in this article and links it to the glossary for a better understanding of that concept in the context of Ayurveda and other topics.
1) Activity:
Activity refers to the effectiveness of the isolated actinomycetes strains in inhibiting the growth of pathogenic bacteria. It is assessed by measuring zones of inhibition during primary and secondary screenings, helping researchers evaluate the antimicrobial potential of soil-derived actinomycetes.
2) Soil:
Soil is the primary source of actinomycetes isolated in this study. Soil samples from paddy fields in Pudipatla near Tirupati, India, were collected, processed, and used to isolate different actinomycetes, which were then screened for their antibiotic-producing capabilities.
3) Antibiotic (Antibacterial):
Antibacterial describes the property of the compounds produced by the actinomycetes that inhibit the growth of bacterial pathogens such as E. coli, B. subtilis, and S. aureus, which are tested through various screening methods.
4) Pramoda (Pramodā):
Pramoda refers to J. Pramoda Kumari, one of the authors of the study, who contributed to the research on isolating and characterizing actinomycetes from paddy soil to find new antimicrobial agents.
5) Kumari (Kumārī, Kumāri):
Kumari is part of the full name of the lead author J. Pramoda Kumari, who is affiliated with the Department of Microbiology at Sri Venkateswara University, and played a significant role in the research presented in the World Journal of Pharmaceutical Research.
6) Substance:
Substance refers to the antimicrobial compounds isolated from the actinomycetes. The study involves characterizing these substances using methods like Thin Layer Chromatography (TLC) to determine their Rf values and bioactivity.
7) Glass:
Glass refers to the container used for Thin Layer Chromatography. A glass beaker was used to hold the solvent for developing TLC plates, which helps in analyzing the antibacterial compounds extracted from actinomycetes.
8) Purification:
Purification is the process of isolating and refining the antibiotic compounds from the actinomycetes. Techniques like Thin Layer Chromatography (TLC) and bioautography are employed to purify and identify the active substances.
9) Discussion:
Discussion is the section of the study where the results are interpreted and compared with existing research. It provides an analysis of the effectiveness of the isolated actinomycetes and their potential as new sources of antibiotics.
10) Habitat:
Habitat refers to the natural environment of the actinomycetes. In this study, soil from paddy fields around Tirupati, Andhra Pradesh, was used to isolate actinomycetes, highlighting the ecological niche from which these antibiotic producers were sourced.
11) Water:
Water is part of the solvent system used in Thin Layer Chromatography (TLC). Specifically, a mixture of ethyl acetate, methanol, and water is utilized to separate the compounds, which are then analyzed for their antimicrobial properties.
12) Blood:
Blood is mentioned as a common bacteriological medium for growing actinomycetes. While blood agar can be used for culturing various bacteria, this study focuses on agar types like starch casein agar for isolating actinomycetes.
13) Drug:
Drug refers to the potential new antibiotics derived from the isolated actinomycetes. The study aims to discover and characterize compounds that can contribute to the development of new therapeutic drugs, addressing the growing issue of bacterial resistance.
Other Science Concepts:
Discover the significance of concepts within the article: �Isolation and characterization of paddy soil actinomycetes for antimicrobial properties�. Further sources in the context of Science might help you critically compare this page with similair documents:
Protective action, Andhra Pradesh, Antibacterial activity, Antimicrobial activities, TLC analysis, Thin Layer Chromatography, Antimicrobial activity, Zone of inhibition, Minimum inhibitory concentration, Microbial pathogens, Inhibition zone, Minimum inhibitory concentration (MIC), Experimental results, Antagonistic activity, Well diffusion method, Bacterial growth, E. coli, Solvent extraction, Biochemical characterization, Gram staining, S. aureus, Secondary screening, Gram positive organism, Nutrient agar plate, Antibacterial compound.
Concepts being referred in other categories, contexts and sources.