Therapeutic impact of bone marrow mesenchymal stem cells in liver fibrosis
Journal name: World Journal of Pharmaceutical Research
Original article title: Therapeutic implications of bone marrow mesenchymal stem cells in experimental liver fibrosis
The WJPR includes peer-reviewed publications such as scientific research papers, reports, review articles, company news, thesis reports and case studies in areas of Biology, Pharmaceutical industries and Chemical technology while incorporating ancient fields of knowledge such combining Ayurveda with scientific data.
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Hanaa H. Ahmed, Magdy S. Amer, Neveen A. Salem, Hebatullah M. Abou El-Fadl
World Journal of Pharmaceutical Research:
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Full text available for: Therapeutic implications of bone marrow mesenchymal stem cells in experimental liver fibrosis
Source type: An International Peer Reviewed Journal for Pharmaceutical and Medical and Scientific Research
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Summary of article contents:
Introduction
Liver fibrosis is a severe condition characterized by excessive extracellular matrix (ECM) accumulation leading to scar formation and significant morbidity. Current treatments are limited, often lacking efficacy in humans and necessitating liver transplants. This study evaluates the therapeutic potential of bone marrow-derived mesenchymal stem cells (BM-MSCs) for managing liver fibrosis using a rat model.
Therapeutic Potential of BM-MSCs
Bone marrow mesenchymal stem cells (BM-MSCs) were harvested, characterized, and tested for their therapeutic efficacy on liver fibrosis. Morphological and gene expression analyses confirmed their identity. BM-MSCs demonstrated a significant ability to improve liver functions, highlighted by reduced serum aspartate aminotransferase (AST) and alanine aminotransferase (ALT) levels, decreased serum fibronectin, and elevated serum albumin levels in treated rats compared to controls. This improved biochemical profile was corroborated by histological findings, showing reduced fibrosis and inflammation.
Mechanism of Action: TGF-β and HGF Modulation
Treatment with BM-MSCs resulted in a marked modulation of key fibrotic and regenerative markers. The study found a significant reduction in hepatic transforming growth factor β (TGF-β) levels in BM-MSCs treated groups, indicating a suppression of fibrogenic activity. Concurrently, an increase in hepatic hepatocyte growth factor (HGF) levels was observed, promoting tissue repair and regeneration. These results suggest that BM-MSCs exert their therapeutic effects through the downregulation of fibrotic pathways and the enhancement of regenerative mechanisms in liver tissue.
Cell Homing and Histopathological Changes
The study also investigated the homing ability of BM-MSCs to the injured liver. PKH-stained BM-MSCs were successfully engrafted in the liver tissue of treated rats, illustrating effective targeting and integration into the damaged sites. Histological examinations supported these findings, showing significant reductions in fibrosis, cholestasis, and improved hepatic architecture in BM-MSC-treated groups. These results not only confirm the homing efficiency of BM-MSCs but also their capability to mitigate histopathological damage induced by thioacetamide.
Conclusion
The study demonstrates the substantial therapeutic potential of BM-MSCs in treating experimental liver fibrosis. BM-MSCs restore liver function through ECM organization and secretion of regenerative factors like HGF, thus positioning themselves as a promising remedy for human liver fibrosis. This research paves the way for further exploration and potential clinical application of BM-MSCs in managing liver-related diseases.
Glossary definitions and references:
Scientific and Ayurvedic Glossary list for “Therapeutic impact of bone marrow mesenchymal stem cells in liver fibrosis�. This list explains important keywords that occur in this article and links it to the glossary for a better understanding of that concept in the context of Ayurveda and other topics.
1) Water:
Water is a critical element in the study as it was used for washing and preparing specimens for histopathological investigation, as seen in the methodology section where tap water was used after fixation in formalin saline (10%) to ensure proper dehydration and clearing processes.
2) Accumulation (Accumulating, Accumulate):
Accumulated relates to the extracellular matrix components that build up during liver fibrosis. The study tracks changes in these accumulated components, like fibronectin, as a measure of disease progression and therapeutic effectiveness of BM-MSCs.
3) Activity:
Activity refers to the enzymatic actions measured in the study, particularly serum AST and ALT activities, which are indicators of liver function and hepatocellular injury. The study shows how BM-MSCs treatment reduced these enzyme activities, indicating improvement in liver condition.
4) Transformation (Transform, Transforming):
Transforming is part of the term 'Transforming Growth Factor-β (TGF-β)', a profibrogenic cytokine central to the study. TGF-β plays a key role in the development and progression of liver fibrosis, making it a critical target for evaluating the effectiveness of BM-MSCs therapy.
5) Medicine:
Medicine is integral to the study, which investigates the therapeutic application of bone marrow mesenchymal stem cells (BM-MSCs) in treating liver fibrosis. The study involves pharmacological and medical approaches to understand cell-based treatments.
6) Science (Scientific):
Science underlies the entire research as it employs scientific methods to isolate, culture, and test the efficacy of BM-MSCs in therapeutic applications for liver fibrosis. The research aligns with biomedical science protocols for experimental and therapeutic studies.
7) Repair:
Repair is a central theme, as the study focuses on how BM-MSCs can aid in the repair and regeneration of liver tissue damaged by fibrosis. The cells promote tissue repair by secreting growth factors like HGF, which support liver regeneration.
8) Veterinary medicine:
Veterinary medicine is relevant as two authors are affiliated with the Faculty of Veterinary Medicine. The study uses animal models (rats) to investigate liver fibrosis treatments, which is a common practice in veterinary pharmacology and pathology.
9) Inflammation:
Inflammation features prominently in the study; liver fibrosis involves inflammatory responses, as seen in the histopathological findings of inflammatory cell infiltration in liver tissue. BM-MSCs treatment reduces inflammation, showcasing their therapeutic potential.
10) Pharmacology:
Pharmacology is a key discipline in the study, as it deals with the actions of BM-MSCs as a therapeutic intervention. Authors from the Pharmacology Department have investigated how these stem cells impact liver fibrosis pharmacodynamically.
11) Malnutrition:
Malnutrition is mentioned as a potential factor contributing to hypoalbuminemia in liver fibrosis. The study notes how liver damage can result in malnutrition and malabsorption, further complicating the fibrotic condition and liver function.
12) Purification:
Purification is significant in the context of isolating total RNA from cultured cells, which is a biochemical procedure to confirm the identity and functionality of BM-MSCs. Purified RNA is crucial for accurate gene expression analysis.
13) Discussion:
Discussion is a sectional heading in the article where the authors interpret their findings, compare them to existing literature, and offer insights into the implications of their study, highlighting the therapeutic potential of BM-MSCs for liver fibrosis.
14) Varsha (Varṣ�, Varṣa, Vārṣa):
Varsha is mentioned in a reference citation, specifically in the context of a study by Mehul and Varsha that examines the effects of thioacetamide on liver function. This reference supports findings related to hyperammonemia and liver damage.
15) Glass:
Glass slides are used in the histopathological investigation of liver tissues, where sections from paraffin-embedded liver samples are collected on glass slides, deparaffinized, stained, and then examined to assess the histological impact of treatments.
16) Blood:
Blood samples are critical in the study for biochemical assays. Serum and plasma from blood samples were used to measure enzymatic activities, ammonia, albumin, fibrinogen, and fibronectin levels, providing insight into liver function and treatment efficacy.
17) Eagle:
Eagle refers to 'Dulbecco's modified Eagle's medium (DMEM)', a culture medium used for harvesting and preparing bone marrow mesenchymal stem cells. It's essential for cell culture, ensuring the cells have the necessary nutrients for growth and differentiation.
18) Diet:
Diet of experimental rats consisted of a specified formula with casein, salts, vitamins, corn oil, and other nutrients, which is crucial for maintaining the health of rats during the study and ensuring that nutritional variables do not confound the results.
19) Hand:
Hand refers to one of the authors, Handley, cited in a method for estimating plasma fibrinogen level using ELISA technique. The contribution by Handley's method supports the biochemical analyses conducted in the study.
20) Salt (Salty):
Salt is part of the diet given to experimental rats. It is also mentioned in the histopathological process where formalin saline is used to fix liver samples. This is crucial for preserving tissue morphology for subsequent analysis.
Other Science Concepts:
Discover the significance of concepts within the article: �Therapeutic impact of bone marrow mesenchymal stem cells in liver fibrosis�. Further sources in the context of Science might help you critically compare this page with similair documents:
Therapeutic application, Insulin, Therapeutic role, Statistical analysis, Oxidative stress, Extracellular matrix, Dexamethasone, Liver Function, Vascular endothelial growth factor, Serum albumin, Real-time PCR, Hepatic damage, Serum aspartate aminotransferase, Collagen deposition, Cell surface markers, Matrix metalloproteinase-9, Tissue repair, Glycosaminoglycans, Hypoalbuminemia, Mesenchymal stem cells, Liver fibrosis, Transforming growth factor beta, Hepatocyte growth factor, Histological investigation, Serum fibronectin level, Cell therapy, Nucleated cells, Serum albumin level, Experimental animal, Biochemical Assay, Serum enzymes, Extracellular matrix components, Inflammatory cells infiltration, Osteogenic differentiation, Adipocyte differentiation, Hyperammonemia, Positive expression, Hepatic fibrosis, Adipose-derived stem cells, Microscopic investigation, Forward primer, Reverse primer, Chondrogenic differentiation, CDNA, Antiapoptotic effect, Plasminogen activator inhibitor-1.
Concepts being referred in other categories, contexts and sources.