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Anxiolytic activity of cannabis sativa extract in albino mice

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Journal name: World Journal of Pharmaceutical Research
Original article title: Pharmacological evaluation of anxiolytic activity of cannabis sativa extract in albino mice
The WJPR includes peer-reviewed publications such as scientific research papers, reports, review articles, company news, thesis reports and case studies in areas of Biology, Pharmaceutical industries and Chemical technology while incorporating ancient fields of knowledge such combining Ayurveda with scientific data.
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Author:

Dheeraj Dubey, Avadh Biharee, Arpita Yadav, Mohit Shrivastava and Dr. Kamal Kishore Maheshwari


World Journal of Pharmaceutical Research:

(An ISO 9001:2015 Certified International Journal)

Full text available for: Pharmacological evaluation of anxiolytic activity of cannabis sativa extract in albino mice

Source type: An International Peer Reviewed Journal for Pharmaceutical and Medical and Scientific Research

Copyright (license): WJPR: All rights reserved


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Summary of article contents:

Introduction

Anxiety disorders are a significant mental health issue, affecting approximately 33.7% of the population at some point in their lives. These disorders encompass a range of conditions characterized by excessive emotional responses to perceived threats, leading to various psychological symptoms. The study conducted by Dubey et al. (2021) aimed to evaluate the anxiolytic effects of ethanolic extracts of Cannabis sativa in albino mice. Using the marble burying apparatus, the research compared the anxiolytic activity of Cannabis sativa with that of the established anxiolytic drug, diazepam.

The Pharmacological Effects of Cannabis sativa

The primary focus of the study was to understand the anxiolytic properties of Cannabis sativa and its potential as an alternative treatment for anxiety disorders. The research involved administering various doses (10 mg/kg, 20 mg/kg, and 40 mg/kg) of ethanolic extract of Cannabis sativa to different groups of mice and observing their behavior in the marble burying test. The results indicated that increasing doses of Cannabis sativa significantly decreased the number of marbles buried, suggesting anxiolytic effects. The effectiveness of the extract was notably lower than diazepam but exhibited potential for inclusion in treatment strategies for anxiety disorders.

Behavioral Assessment Using the Marble Burying Apparatus

The marble burying test serves as a reliable method for assessing anxiety-like behavior in rodents. In this study, the amount of marbles buried by the mice was recorded as a measure of anxiety, where fewer marbles buried indicated lower anxiety levels. The results showed that mice treated with increasing doses of Cannabis sativa extracted exhibited a reduction in marble burying compared to the control group treated with normal saline. This tool not only highlights behavioral changes but also provides insights into the pharmacological effects of potential anxiolytic compounds, establishing a clear methodology for such evaluations.

Comparative Effectiveness of Diazepam and Cannabis sativa

A significant part of the findings emphasized the comparison between the effects of Cannabis sativa and the standard anxiolytic drug diazepam. Diazepam, administered at 2 mg/kg, demonstrated a more substantial reduction in marble burying than either of the Cannabis sativa doses. The study found that while the 20 mg/kg dose of extract showed some anxiolytic effects, the 40 mg/kg dose produced the most pronounced effect among the Cannabis sativa groups, albeit still less effective than diazepam. These results underscore that while Cannabis sativa shows potential, it may not yet match the efficacy of existing anxiolytic treatments.

Conclusion

The research by Dubey et al. (2021) revealed that the ethanolic extract of Cannabis sativa possesses anxiolytic properties, as evidenced by significant decreases in marble burying behavior in mice. However, the efficacy of the extract was found to be inferior to that of diazepam. The dosage that provided the most notable anxiolytic effect was 40 mg/kg, suggesting a potential role for Cannabis sativa in anxiety management strategies. Overall, while Cannabis sativa exhibits promise, further research and clinical trials are necessary to fully explore its therapeutic potential and safety for treating anxiety disorders.

FAQ section (important questions/answers):

What was the purpose of the study on Cannabis sativa?

The study aimed to evaluate the anxiolytic effects of the ethanolic extract of Cannabis sativa in mice, using the marble burying apparatus to assess behavioral changes.

How was the anxiolytic activity evaluated in the study?

The anxiolytic activity was evaluated using the marble burying apparatus, where the number of marbles buried by mice was measured after treatment with different doses of Cannabis sativa extract.

What groups were used in the experimental design?

Mice were divided into five groups: normal saline, diazepam, and three different doses of Cannabis sativa extract (10, 20, and 40 mg/kg).

Which doses of Cannabis sativa showed significant anxiolytic effects?

The study found that doses of 20 mg/kg and 40 mg/kg of Cannabis sativa showed significant anxiolytic effects, with decreased marble burying compared to the control.

How does the effect of Cannabis sativa compare to diazepam?

While the ethanolic extract of Cannabis sativa showed some anxiolytic activity, diazepam was more effective, demonstrating stronger anti-anxiety effects in the marble burying test.

What were the key findings of the research on Cannabis sativa?

The research concluded that while Cannabis sativa has potential anxiolytic properties, its efficacy varies by dosage and is less potent than diazepam, particularly at the lower dosage.

Glossary definitions and references:

Scientific and Ayurvedic Glossary list for “Anxiolytic activity of cannabis sativa extract in albino mice�. This list explains important keywords that occur in this article and links it to the glossary for a better understanding of that concept in the context of Ayurveda and other topics.

1) Cannabis:
Cannabis, specifically Cannabis sativa, is a plant recognized for its psychoactive and therapeutic properties. Its relevance in this study lies in its potential anxiolytic effects, explored through an ethanolic extract. The plant has historical significance ranging from medicinal applications to recreational use, prompting scientific inquiry into its pharmacological impacts on anxiety disorders.

2) Marble:
Marble refers to the small spherical objects used in the marble burying apparatus, a behavioral test in this study to measure anxiety. The relevance of how many marbles are buried by the mice serves as an indicator of anxiety levels; fewer buried marbles typically reflect reduced anxiety or increased exploratory behavior.

3) Anxiety:
Anxiety represents a significant mental health issue characterized by heightened fear and stress responses. The relevance of anxiety in this study is crucial as it investigates the anxiolytic potential of Cannabis sativa, addressing an increasingly prevalent disorder that affects a large portion of the population, necessitating effective treatment alternatives.

4) Animal:
Animals, plural of animal, refers to the collective subjects used in experimental settings, such as the mice utilized in this research. The relevance lies in their role in understanding biological and behavioral responses to treatments aimed at alleviating anxiety, as research findings often extrapolate to potential human applications.

5) Activity:
Activity in this context refers to the behavioral responses observed in the mice during the study, particularly related to anxiety levels as measured by the marble-burying assay. The distinction between different levels of activity can indicate the efficacy of the treatment being tested and the overall neuropharmacological impact.

6) Drug:
Drugs encompasses a broader category of substances used to treat medical conditions, which includes both the standard medication (diazepam) and the experimental cannabis extract in the study. The relevance of this term highlights the ongoing search for effective pharmacological interventions for anxiety and the importance of exploring various therapeutic agents.

7) Study (Studying):
The study is a systematic investigation aimed at exploring the anxiolytic effects of Cannabis sativa extract on mice. Its relevance pertains to addressing significant gaps in the pharmacological treatment of anxiety disorders, with findings potentially influencing clinical practices and shaping future research directions in this domain.

8) Pharmacological:
Pharmacological relates to the study of how drugs interact with biological systems. In this context, it highlights the investigation of the pharmacological properties of Cannabis sativa extract and its potential therapeutic effects against anxiety. Understanding these interactions guides the safer development of anxiety treatments and enhances clinical applications.

9) India:
India is the country where this research was conducted, specifically in Uttar Pradesh. Its relevance indicates the cultural and legal context of studying Cannabis sativa, an important aspect when considering traditional practices and contemporary scientific explorations. The geographic setting may influence the availability and strains of the plant used.

10) Fear:
Fears relate to specific anxiety responses toward perceived threats or dangers. The relevance in this study connects to understanding anxiety disorders, particularly how cannabis might mitigate fears, thereby influencing behavior and leading to potential therapeutic avenues in the treatment of anxiety and related disorders.

11) Cage:
Cages signify the housing used for the mice during the study, reflecting the controlled environment necessary for ethical animal research. The relevance of cage design and size promotes understanding behavior, influences stress levels, and mimics natural conditions to obtain valid experimental results in pharmacological studies.

12) Obsession:
Obsession is defined as persistent intrusive thoughts commonly associated with anxiety disorders, especially Obsessive-Compulsive Disorder (OCD). The relevance of obsession in this context highlights the study's purpose to explore potential interventions for anxiety symptoms and related conditions, examining if Cannabis sativa can mitigate compulsive behaviors related to anxiety.

13) Water:
Water signifies the solvent used for diluting compounds and providing hydration to the animals. In experimental settings, ensuring that lab animals have access to clean water is crucial for their health. In this study, distilled water acted as the control solution to compare against the drug treatments administered to the mice.

14) Table:
The table format is used to present data systematically, summarizing experimental groups and results clearly. In the context of the study, tables display the number of marbles buried, enabling straightforward comparison of anxiety levels across different treatment conditions. This presentation aids in clarity and aids the analytical interpretation of results.

15) Depression:
Depression, often associated with anxiety disorders, is another significant mental health concern analyzed in conjunction with anxiety. The relevance here indicates a potential overlap in pharmacological treatments and underscores the complexity of treating co-morbid mental health conditions, highlighting the need for comprehensive strategies that encompass anxiety and depressive symptoms.

16) Genu:
The term 'genu' typically denotes a 'knee' or 'bend' and might refer contextually to anatomical references. However, in relation to this study, it may hold less direct significance as it’s not specifically addressed. Its inclusion may indicate a broader discussion of anatomy relevant to drug action or behavioral responses in pharmacological studies.

17) Post:
Post refers to something that occurs after a certain event. In the context of this study, it might relate to post-experimental observations or results measured after treatment with the Cannabis extract. The relevance emphasizes the need for post-treatment evaluation to accurately gauge the efficacy and temporal effects of anxiolytic interventions.

18) Substance:
Substance broadly indicates any material with substance, in this case, referring to the cannabis extract as a treatment for anxiety. Its relevance lies in distinguishing between various substances used in the study, such as the active compounds in Cannabis sativa, which are crucial for understanding their therapeutic potential and effects.

19) Relative:
Relative indicates a relationship or comparison between two or more factors. In this study context, the term is relevant in discussing how the effects of the cannabis extract relate to those of established anxiety treatments, allowing for a better understanding of its efficacy compared to other interventions like diazepam.

20) Heating:
Heating is part of the extraction process, where cannabis leaves are subjected to heat to obtain the ethanolic extract. The relevance of heating emphasizes the importance of temperature control in extracting active compounds, which influences the quality and efficacy of the resultant extract used in evaluating its pharmacological effects.

21) Family:
Family in a biological context indicates a category that encompasses several genera. Cannabis sativa belongs to the Cannabaceae family. The relevance lies in classifying plants based on genetic and morphological similarities, aiding in the identification and understanding of the properties of Cannabis in relation to other plants within the same family.

22) Glass:
Glass pertains to the materials used in laboratory equipment, such as beakers and flasks, essential for conducting experiments. Its relevance emphasizes the need for proper laboratory tools to ensure safe handling of substances and accurate measurements during the extraction and evaluation phases of the research.

23) Road:
Road may refer to a physical pathway or a metaphorical journey in the context of research progression. Its relevance is less direct within the study but may imply the broader path of medicinal plant research and the exploration of effective treatments for mental health issues like anxiety.

24) Bronchial asthma:
Bronchial asthma is a chronic inflammatory disease of the airways. Its mention suggests that cannabis may have therapeutic implications beyond anxiety, with cannabinoids also being researched for respiratory conditions. The relevance lies in broadening the scope of cannabis properties, linking it to multiple medical applications.

25) Mental disorder:
Mental disorder refers to conditions that affect mood, thinking, and behavior. The relevance is key to this study, focusing on anxiety as a mental disorder. Understanding the prevalence of mental disorders informs the rationale for exploring alternative treatments, like Cannabis sativa, to address various psychiatric conditions.

26) Mental health:
Mental health indicates the overall psychological well-being of an individual. The relevance of mental health in this study is profound, as it underscores the significance of developing effective treatments for anxiety disorders, contributing to the broader public health discourse on promoting mental well-being and addressing the mental health crisis.

27) Pharmacotherapeutics:
Pharmacotherapeutics denotes the field concerned with the study of drugs used in treatment. Its relevance in the study stems from analyzing the therapeutic effects of Cannabis sativa extracts, contributing to knowledge of effective pharmacological interventions for treating anxiety disorders and exploring new avenues for mental health treatment.

28) Arrangement:
Arrangement may refer to the organization of experimental setups or treatments in the research methodology. The relevance involves systematic planning of the experiments conducted to evaluate the anxiolytic effects accurately, ensuring reliability and consistency of results gathered during the study and enabling clear interpretation of outcomes.

29) Srivastava (Sri-vastava, Shrivastava, Shri-vastava):
Srivastava may refer to a key figure in the study or research team, contributing to the research's insights. Recognizing authorship underlines the importance of collaboration in scientific endeavors and may inform readers about the credibility and expertise behind the research findings on Cannabis sativa and its anxiolytic effects.

30) Discussion:
Discussion encompasses the analysis and interpretation of the results obtained during the study. The relevance lies in contextualizing findings within existing literature on anxiety treatments, addressing implications for future research, and exploring the broader impact of the results on understanding Cannabis sativa's role in mental health treatment.

31) Science (Scientific):
Science denotes the systematic pursuit of knowledge through observation and experimentation. Its relevance in this study asserts the structured approach taken to explore the anxiolytic effects of Cannabis sativa, aligning the research with broader scientific inquiries aimed at enhancing mental health treatments and understanding complex biological systems.

32) Suffering:
Suffering relates to the experience of distress and discomfort often associated with anxiety and other mental disorders. The relevance underscores the human impact of anxiety-related conditions, reinforcing the necessity of developing effective treatments, such as the one explored in this study, to alleviate the burdens of those affected.

33) Himalaya (Hima-alaya):
Himalaya, similar to 'Himalayas', relates to the mountain range and its historical significance in the context of herbal medicine. Its inclusion emphasizes the potential traditional knowledge surrounding the use of cannabis in different regions, providing insights into its pharmacological properties and applications influenced by cultural practices.

34) Medicine:
Medicine signifies the field focused on the diagnosis and treatment of illnesses. The relevance in this study includes understanding Cannabis sativa's therapeutic applications for mental health conditions like anxiety. Insights gained here may enhance clinical practices and contribute to the ongoing transformation of treatment paradigms in mental health.

35) Epilepsy:
Epilepsy is mentioned as a condition possibly influenced by cannabis therapy, highlighting the range of medical conditions studied in pharmacology. The relevance underlines the versatility of cannabis compounds in addressing various neurological and psychiatric disorders, prompting further investigation into their broad therapeutic potential.

36) Mathura (Mathur):
Mathura is a town in Uttar Pradesh, India, where part of the research activities were likely conducted. Its mention emphasizes the regional context of the study and may reflect on the local biodiversity and availability of medicinal plants, enhancing the understanding of ethnobotanical practices and their scientific validation.

37) Disease:
Disease encompasses broad categories of health conditions, including mental health disorders that this study directly addresses. The relevance is critical, as understanding disease mechanisms informs the development of effective treatments, with the research exploring how pharmacological interventions can alleviate symptoms of specific mental health conditions, such as anxiety.

38) Potter:
Potter may refer to a researcher or author who contributed to the foundational knowledge about the Cannabis plant discussed in the study. The relevance lies in recognizing prior studies that shape contemporary research, helping to inform the current understanding of cannabis' properties and therapeutic implications.

39) Nature:
Nature refers to the inherent qualities and characteristics of living organisms and the environment. Within this study, the relevance emphasizes understanding the natural properties of Cannabis sativa and its extraction procedures, providing insights into harnessing natural resources for therapeutic purposes and the ecological contexts of medicinal plants.

40) Botany:
Botany, the study of plants, is relevant here as it encompasses the classification, properties, and uses of Cannabis sativa in the research. Understanding botany supports the exploration of medicinal plants' role in pharmacology, informing the mechanisms by which nature’s resources can be effectively utilized in treating health conditions.

41) Sharman (Sarma, Sharma, Sarman):
Sarma likely refers to a researcher or contributor to the existing literature on cannabis discussed in this study. Its relevance highlights the importance of citing previous contributions to scientific knowledge, ensuring that the current research builds upon and acknowledges prior work in exploring the therapeutic potential of Cannabis sativa.

42) Doubt:
Doubt expresses uncertainty or skepticism, often encountered in research as scientists aim to validate findings. Its relevance in this study implies the necessity for rigorous inquiry and systematic approaches to dispel doubts regarding Cannabis sativa's efficacy in treating anxiety, urging careful evaluation of the results against established knowledge.

43) Money:
Money indicates the financial considerations associated with funding research activities or accessing medications. Its relevance touches on the broader discussion around healthcare availability, affordability of treatments, and the economic factors influencing research priorities in mental health and substance use disorders, informing approaches to accessibility of care.

44) House:
House can refer to the physical location where the animals were housed during the research. Its relevance discusses the ethical responsibility to provide a suitable living environment for research animals, which can influence results. Proper housing supports stress-free conditions, essential for humane scientific inquiry.

45) Worry (Worried, Worrying):
Worry is an emotional state characterized by apprehension and unease, closely associated with anxiety. The relevance of worry in this study underscores the need for therapeutic interventions to manage such feelings, emphasizing the implications of developing effective treatments for anxiety that can alleviate chronic worry in affected individuals.

46) Cina:
China signifies a geographic context of natural resource cultivation, including cannabis. Its relevance may concern historical uses of medicinal plants in traditional Chinese medicine, linking cultural practices to the scientific exploration of plants like Cannabis sativa, furthering the understanding of their pharmacological benefits.

47) Crowd:
Crowd symbolizes social environments that may induce anxiety for some individuals, connecting to the themes of social anxiety disorder. Its relevance emphasizes the societal aspects of anxiety, highlighting the importance of addressing various triggers such as public speaking or social settings through research into effective treatment options.

48) Food:
Food signifies basic sustenance for living organisms, underpinning health and well-being. Although indirectly related to the study, its relevance can touch on nutritional aspects affecting mental health and anxiety, illustrating the importance of holistic approaches that include diet as part of comprehensive mental health care.

49) Sign:
Sign may refer to indicators or symptoms associated with anxiety and mental disorders. Its relevance in this study emphasizes the necessity to identify and validate clinical signs of anxiety, enabling clinicians to make informed decisions regarding treatment efficacy and contribute to improved patient outcomes.

50) Pain:
Pain often co-occurs with anxiety disorders, highlighting the overlap of various mental and physical health issues. The relevance of considering pain within this study reflects the broader context of patient experience, where managing anxiety may also alleviate concurrent pain conditions, reinforcing the interconnectedness of mental health and overall well-being.

51) Mud:
Mood refers to a person's emotional state, which can be significantly influenced by anxiety. Its relevance underscores the bidirectional relationship between mood and anxiety disorders, emphasizing the necessity for treatments, such as those using Cannabis sativa, to address emotional well-being and contribute positively to a patient's mental health.

52) Life:
Life encompasses the human experience, filled with various challenges, including mental health struggles such as anxiety. Its relevance in this study reflects on the broader implications of anxiety disorders on quality of life, emphasizing the need for effective treatments to help individuals lead healthier, more fulfilling lives.

Other Science Concepts:

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Discover the significance of concepts within the article: �Anxiolytic activity of cannabis sativa extract in albino mice�. Further sources in the context of Science might help you critically compare this page with similair documents:

Cannabis sativa, Clinical feature, Post-traumatic stress disorder, Generalized Anxiety Disorder, Anxiolytic activity, Standard drug diazepam, Pharmacological treatment, Obsessive-Compulsive Disorder, Rodent model, Dosage level, Research paradigm.

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