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Ethanolic polyherbal extract protects against CCl4 liver injury

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Journal name: Ancient Science of Life
Original article title: Protective effect of ethanolic extract of polyherbal formulation on carbon tetrachloride induced liver injury
The ANSCI is a peer-reviewed, open-access journal focused on Ayurveda and traditional medicines. It publishes original research, reviews, and literary studies linking traditional knowledge with modern science, covering disciplines like botany, ethnomedicine, pharmacology, and clinical research.
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Original source:

This page is merely a summary which is automatically generated hence you should visit the source to read the original article which includes the author, publication date, notes and references.

Author:

K Gurusamy, R Kokilavani, K Arumugasamy, C Sowmia


Ancient Science of Life:

(A quarterly multi-disciplinary scientific research journal in Ayurveda)

Full text available for: Protective effect of ethanolic extract of polyherbal formulation on carbon tetrachloride induced liver injury

Year: 2009

Copyright (license): CC BY-NC-SA


Download the PDF file of the original publication


Summary of article contents:

Introduction

Liver disease poses significant health challenges, and the search for effective liver-protective medications in conventional medicine has highlighted the potential of herbal remedies. This study investigates the hepatoprotective properties of a polyherbal formulation (PHF) made from three medicinal plants: Asteracantha longifolia, Cyperus rotundus, and Bryophyllum pinnatum. The effects of this formulation on liver injury induced by carbon tetrachloride (CCl�) in rats were evaluated, utilizing various biochemical markers to assess liver function and damage.

Antioxidant Mechanism in Hepatoprotection

The mode of action underlying the hepatoprotective effects of the PHF is strongly linked to its antioxidant properties. Carbon tetrachloride is known to cause oxidative damage in liver cells through the generation of free radicals, leading to lipid peroxidation and subsequent cellular injury. The PHF's active components, including flavonoids and terpenoids, are believed to scavenge these free radicals, thereby reducing oxidative stress in liver tissues. Experimental results demonstrated that treatment with the PHF significantly lowered the elevated serum levels of specific liver enzymes (5'NT, GGT, GDH, and SDH) and bilirubin, indicating a normalization of liver function following CCl� exposure.

Conclusion

The findings from this study indicate that the ethanolic extract of the polyherbal formulation offers significant hepatoprotective benefits, effectively mitigating liver damage induced by CCl� in rat models. The ability of the PHF to restore biochemical markers to normal levels underscores its potential as a therapeutic agent in the management of liver disorders. Future studies may explore the specific active compounds within the formulation that contribute to its protective effects, as well as the application of this knowledge in clinical settings for the treatment of liver diseases.

FAQ section (important questions/answers):

What is the purpose of the study on polyherbal formulation?

The study evaluates the hepatoprotective activity of an ethanolic extract of a polyherbal formulation against liver damage induced by carbon tetrachloride in rats.

Which plants were used in the polyherbal formulation preparation?

The polyherbal formulation consisted of Asteracantha longifolia, Cyperus rotundus, and Bryophyllum pinnatum, collected from different districts of Tamilnadu, India.

How was hepatotoxicity induced in the experimental rats?

Hepatotoxicity was induced by intraperitoneal administration of carbon tetrachloride at a dose of 1.0 ml per kg body weight, mixed with liquid paraffin as a vehicle.

What were the main biochemical parameters measured in the study?

The study measured serum constituents and enzyme activities, including 5'NT, GGT, GDH, SDH, and bilirubin levels to assess liver damage and hepatoprotection.

Glossary definitions and references:

Scientific and Ayurvedic Glossary list for “Ethanolic polyherbal extract protects against CCl4 liver injury�. This list explains important keywords that occur in this article and links it to the glossary for a better understanding of that concept in the context of Ayurveda and other topics.

1) Activity:
Activity refers to the biological effects or actions that substances, such as the ethanolic extract of the polyherbal formulation, exhibit in mitigating or reversing liver damage induced by toxins like carbon tetrachloride (CCl4). This is measured by various biochemical markers in serum and liver tissues, indicating effectiveness against liver injury.

2) Science (Scientific):
Science is the systematic study of the natural world, emphasizing observation, experimentation, and evidence-based understanding. In the context of this research, it underpins the methodologies used in evaluating the hepatoprotective properties of herbal formulations, showcasing the importance of empirical data in medicinal plant studies and pharmacology.

3) Shukla (Sukla):
Shukla refers to one of the authors mentioned in the text whose research contributes to the understanding of hepatoprotective activities and antioxidant effects of plant extracts. Collaborations in scientific research, like those of Shukla, validate and advance knowledge regarding natural remedies against liver diseases.

4) Botany:
Botany is the branch of biological sciences concerned with the study of plants, including their structure, properties, and biochemical processes. It is relevant here as it encompasses the identification and classification of the medicinal plants used in the polyherbal formulation, which plays a crucial role in their therapeutic exploration.

5) Drug:
A drug is a substance used for medical treatment, which can affect physiological functions. In the context of this study, it contrasts the herbal formulations being explored as alternatives to conventional allopathic medicines for liver protection, highlighting the historical and ongoing search for effective treatments using natural compounds.

6) Flavonoid:
Flavonoids are a class of polyphenolic compounds commonly found in plants, known for their antioxidant properties. They are crucial in the study because their presence in the ethanolic extract from the polyherbal formulation contributes to the observed hepatoprotective effects against chemical-induced liver damage, making them relevant targets for research.

7) Sharman (Sarma, Sharma, Sarman):
Sharma is another contributor to the research detailed in the text. His involvement likely emphasizes collaborative efforts in scientific studies, aiding in the exploration of medicinal plants' potential health benefits. It highlights the significance of teamwork in advancing knowledge about hepatoprotective properties in traditional medicine.

8) Water:
Water is a vital solvent and transport medium in biological organisms. In the context of the research, it is used in preparing solutions for administering extracts of medicinal plants, which is critical for studying their effects on liver functions, underscoring its fundamental role in biochemical and physiological processes.

9) Blood:
Blood serves as the medium through which nutrients, waste, and biochemical signals are transported in the body. In this research, blood samples were analyzed to determine the serum levels of liver enzymes and bilirubin after treatment with the polyherbal formulation, providing insight into its effectiveness against liver toxicity.

10) Cyperus rotundus:
Cyperus rotundus, commonly known as nut grass, is one of the plants used in the polyherbal formulation. Its inclusion is significant due to its traditional use in various systems of medicine for potential health benefits, including hepatoprotective effects, which are explored through experimental models in this study.

11) Pharmacology:
Pharmacology is the science concerned with the study of drug action. The research engages pharmacological principles by assessing the effects of the polyherbal formulation on liver injury, highlighting the significance of medicinal plants and their constituents within the broader context of drug development and therapeutic applications.

12) Discussion:
Discussion refers to the section of scientific research where results are interpreted and implications analyzed. In this study, it provides insights into the findings related to the hepatoprotective effects of the herbal formulation, connecting experimental data with potential applications in traditional and modern medical practices.

13) Toxicity:
Toxicity refers to the degree to which a substance can cause harm to an organism. The research focuses on the hepatotoxic effects of carbon tetrachloride, investigating how the polyherbal formulation mitigates these toxic effects on the liver, thereby establishing its relevance in treating chemical-induced liver injuries.

14) Cancer:
Cancer represents a broad spectrum of diseases characterized by uncontrolled cell proliferation. Although the study primarily targets liver injury, the underlying principles of investigating oxidative stress and cellular damage have parallels in cancer research, emphasizing the importance of antioxidant agents in holistic health and disease prevention.

15) Gupta:
Gupta is another researcher referenced in the study, likely contributing to the body of knowledge regarding hepatoprotective properties of plant extracts. The collaborative nature of research is emphasized, illustrating how individual contributions lead to comprehensive understanding and advancements in natural product pharmacology.

16) Latta:
Latha denotes one of the researchers involved in the study. Her contributions to the research signify the importance of gender diversity and collective expertise in science, essential for exploring the therapeutic potential of herbal formulations and analyzing their biochemical impacts in health and disease.

17) Mica:
Mica, commonly known as a mineral, does not have direct relevance in the provided excerpt. However, it can represent broader discussions around natural resources or materials involved in chemical analyses in scientific research, emphasizing the importance of purity in materials used during experimental procedures.

18) Diet:
Diet pertains to the customary intake of food and drinks by organisms. In this study, the standard diet provided to experimental rats is crucial for ensuring consistent nutritional conditions during the experiment, allowing for reliable assessment of the polyherbal formulation's effects on liver health amid controlled dietary variables.

Other Science Concepts:

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Discover the significance of concepts within the article: �Ethanolic polyherbal extract protects against CCl4 liver injury�. Further sources in the context of Science might help you critically compare this page with similair documents:

Natural product, Polyherbal formulation, Hepatotoxicity, Hepatoprotective activity, Lipid peroxidation, Animal Ethics Committee, Carbon tetrachloride, Ethanolic extract, Liver disease, Bilirubin level, Free Radical, Gamma Glutamyl Transferase, Succinate dehydrogenase, Glutamate dehydrogenase, Liver protective drugs, Trichloromethyl free radical.

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