Antimicrobial activity of callus vs. natural extracts of Solanum trilobatum
Journal name: Ancient Science of Life
Original article title: Comparative antimicrobial activity of callus and natural plant extracts of Solanum trilobatum L
The ANSCI is a peer-reviewed, open-access journal focused on Ayurveda and traditional medicines. It publishes original research, reviews, and literary studies linking traditional knowledge with modern science, covering disciplines like botany, ethnomedicine, pharmacology, and clinical research.
This page presents a generated summary with additional references; See source (below) for actual content.
Original source:
This page is merely a summary which is automatically generated hence you should visit the source to read the original article which includes the author, publication date, notes and references.
S.M. Nagarajan, S. Kandasamy, R. Chinnappa
Ancient Science of Life:
(A quarterly multi-disciplinary scientific research journal in Ayurveda)
Full text available for: Comparative antimicrobial activity of callus and natural plant extracts of Solanum trilobatum L
Year: 2009
Copyright (license): CC BY-NC-SA
Download the PDF file of the original publication
Summary of article contents:
Introduction
Solanum trilobatum L., a medicinal plant in the Solanaceae family, is valued for its therapeutic properties, including the treatment of cough, chronic bronchitis, and various gastrointestinal issues. The plant's roots and leaves are known to be bitter and astringent, and they are often used in traditional medicine to alleviate respiratory problems. Due to the overharvesting of natural populations, tissue culture techniques have been explored to facilitate the rapid multiplication and conservation of this valuable species. The study aimed to assess the antimicrobial activity of callus and natural extracts of S. trilobatum against specific bacteria and fungi using various solvents.
Enhanced Antimicrobial Activity of Callus Extracts
The research indicated that callus extracts, particularly derived from stem and leaf tissues cultured on Murashige and Skoog medium with specific growth regulators (0.5 mg/l NAA and 0.5 mg/l Kinetin), exhibited significantly higher antimicrobial activity than natural extracts. The study employed the cup diffusion method to test the extracts against Escherichia coli and Staphylococcus aureus for antibacterial properties, and Aspergillus flavus and Aspergillus niger for antifungal properties. The results showed that the petroleum ether and chloroform extracts from the leaf callus were most effective against the tested fungi. This enhanced antimicrobial effect in callus extracts is attributed to a higher concentration of secondary metabolites compared to those found in natural extracts.
Conclusion
In conclusion, the study demonstrates that callus cultures of Solanum trilobatum can serve as a potent source of antimicrobial compounds, making them potentially superior to naturally sourced extracts. The findings suggest that further research into the isolation and purification of specific active compounds from callus could yield significant insights and advancements in pharmacological applications. This underlines the importance of plant tissue culture not only for conservation but also for enhancing the availability of bioactive compounds with therapeutic potential.
FAQ section (important questions/answers):
What is Solanum trilobatum and its significance?
Solanum trilobatum L. is a medicinal plant from the Solanaceae family. It's used for treating respiratory issues, rheumatism, and gastric problems. The extracts from its leaves and roots are known for their antibacterial and antifungal properties.
What tissue culture technique was used for the study?
The study utilized callus induction from stem and leaf explants on MS medium enriched with growth regulators like NAA and Kinetin. This method promotes rapid multiplication and conservation of the plant's valuable genetic material.
How was the antimicrobial activity of extracts assessed?
Antimicrobial activity was evaluated using the cup diffusion method on bacteria like E. coli and Staphylococcus aureus, as well as fungi such as Aspergillus flavus and Aspergillus niger. Various solvent extracts were tested for their effectiveness.
What were the results of the antimicrobial tests?
The results indicated that callus extracts, particularly from leaves and stems, exhibited significant antimicrobial activity compared to natural extracts. Petroleum ether and chloroform extracts showed the highest inhibitory effects against tested microorganisms.
Glossary definitions and references:
Scientific and Ayurvedic Glossary list for “Antimicrobial activity of callus vs. natural extracts of Solanum trilobatum�. This list explains important keywords that occur in this article and links it to the glossary for a better understanding of that concept in the context of Ayurveda and other topics.
1) Activity:
The term 'Activity' in this context refers to the biological effectiveness of extracts from Solanum trilobatum, specifically their antimicrobial properties against certain bacteria and fungi. This activity is measured through assays that show how different extracts inhibit microbial growth, which is crucial for evaluating the therapeutic potential of the plant.
2) Antibiotic (Antibacterial):
Antibacterial describes substances or properties that inhibit the growth of bacteria. In this study, the antibacterial efficacy of the extracts is pivotal for assessing the medicinal value of Solanum trilobatum, particularly its potential use in treating bacterial infections. The results indicate significant antibacterial activity, making it relevant for healthcare applications.
3) Science (Scientific):
Science encompasses the systematic study and understanding of natural phenomena. In this research, scientific methods, such as tissue culture and antimicrobial assays, are utilized to explore the medicinal properties of Solanum trilobatum. This scientific inquiry contributes to the broader field of pharmacognosy, which investigates the potential health benefits of natural products.
4) Botany:
Botany, the study of plants, is central to this research since it focuses on Solanum trilobatum, a plant of significant medicinal value. Understanding the biology, growth, and extraction processes of the plant aids in effective cultivation and application of its health benefits, linking theoretical knowledge to practical use in medicine.
5) Water:
Water is a vital solvent used in the sterilization and extraction processes of the plant material. The cleaning steps involve rinsing explants in water as a preparatory measure. Furthermore, water serves as a medium for dissolving extracts, highlighting its fundamental role in facilitating the methodologies employed for this research.
6) Purification:
Purification pertains to the refined process of isolating specific active compounds from plant extracts. The study emphasizes the need for purification to identify and enhance bioactive components with antimicrobial properties. This step is essential for future investigations aimed at developing pharmaceutical products derived from Solanum trilobatum.
7) Discussion:
Discussion refers to the section where the findings from the study are interpreted and related to existing literature. It serves to contextualize the results, draw conclusions about the effectiveness of the extracts, and suggest avenues for further research, thereby contributing to the understanding of the plant's medicinal properties.
8) Gingelly (Gingelli):
Gingelly oil is mentioned in the context of traditional uses of Solanum trilobatum in cooking and medicinal preparations. Its relevance highlights the cultural and culinary significance of the plant as well as its integration into local remedies for treating health issues, showing the interplay between gastronomy and herbal medicine.
9) Bitter:
Bitter is an adjective describing the taste of certain parts of Solanum trilobatum, like its leaves and roots. The bitterness is attributed to the presence of particular compounds, which may also contribute to its medicinal properties, providing a link between taste, phytochemistry, and therapeutic effects in herbal medicine.
10) Anna:
Anna refers to Arignar Anna Government Arts College, part of the affiliation where the research is conducted. The institution plays a role in advancing knowledge in botany and medicinal plant studies, indicating the educational context within which this research is situated and its contribution to academic discourse.
Other Science Concepts:
Discover the significance of concepts within the article: �Antimicrobial activity of callus vs. natural extracts of Solanum trilobatum�. Further sources in the context of Science might help you critically compare this page with similair documents:
Antibacterial activity, Zone of inhibition, Antifungal activity, Secondary metabolite, Soxhlet extraction, Explant, Tissue culture technique, Murashige and Skoog medium, Dimethyl Formamide (DMF), Solanum trilobatum L., Callus extracts, Natural plant extracts, Growth regulator.