Anti leukaemic activity of evolvulus alsinoides
Journal name: World Journal of Pharmaceutical Research
Original article title: Anti leukaemic activity of evolvulus alsinoides
The WJPR includes peer-reviewed publications such as scientific research papers, reports, review articles, company news, thesis reports and case studies in areas of Biology, Pharmaceutical industries and Chemical technology while incorporating ancient fields of knowledge such combining Ayurveda with scientific data.
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N V L Sirisha Mulukuri, Omkar singh, Sukhdeb Baenerje
World Journal of Pharmaceutical Research:
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Full text available for: Anti leukaemic activity of evolvulus alsinoides
Source type: An International Peer Reviewed Journal for Pharmaceutical and Medical and Scientific Research
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Summary of article contents:
Introduction
Evolvulus alsinoides, commonly known as Shankhpushpi, is a notable medicinal plant in Ayurveda recognized for its versatile therapeutic properties. This study investigates its antileukemic activity by isolating and characterizing compounds from its leaf extract and evaluating their cytotoxic effects on different leukemic cell lines.
Isolation and Identification of Bioactive Compounds
The study employed successive solvent extraction using petroleum ether, chloroform, and methanol to isolate bioactive compounds from the dried leaves of Shankhpushpi. Through chromatographic purification and spectral analysis, four distinct compounds were identified: 尾-sitosterol-3-O-glucoside, 尾-sitosterol, Ursolic acid, and Kaempferol-3, 7-di-O-rhamnoside, designated as OS-1, OS-2, OS-3, and OS-4 respectively. These identifications were confirmed by comparing spectral data with known literature.
Cytotoxicity Assay Using MTT
The isolated compounds OS-3 and OS-4 were tested for their cytotoxicity against leukemic cell lines U937, K562, and HL60 using the MTT assay. The results indicated that both OS-3 and OS-4 exhibit significant cytotoxic effects, with OS-4 (Kaempferol-3, 7-di-O-rhamnoside) showing the highest cytotoxicity, followed by OS-3 (Ursolic acid). The assay involved measuring the optical density to determine the degree of cytotoxicity, revealing a dose-dependent inhibition of cell viability.
Effectiveness Against Leukemic Cell Lines
The cytotoxic effect of the compounds was further broken down by their specific impacts on different leukemic cell lines. OS-4 demonstrated higher effectiveness across all tested cell lines (HL60, K562, U937) compared to OS-3. This indicates a potential for Kaempferol-3, 7-di-O-rhamnoside as a potent anti-leukemic agent. The observed cytotoxic effects suggest that the compounds may invoke cell death through apoptosis or necrosis, necessitating further studies to delineate the exact pathways involved.
Conclusion
This research concludes that Evolvulus alsinoides contains bioactive compounds with significant cytotoxic effects against leukemic cells. Of particular note are Kaempferol-3, 7-di-O-rhamnoside (OS-4) and Ursolic acid (OS-3), which showed promising antileukemic activities. These findings underscore the potential of these compounds to be developed as therapeutic agents for leukemia, with further analysis required to understand their mechanisms of inducing cell death.
FAQ section (important questions/answers):
What is the main study focus of this research?
The study focuses on the antileukemic activity of compounds isolated from Evolvulus alsinoides (Shankhpushpi).
What solvents were used for extraction?
The plant leaves were extracted using petroleum ether, chloroform, and methanol successively.
Which compounds were identified from the extracts?
Compounds identified include 尾-sitosterol-3-O-glucoside, 尾-sitosterol, Ursolic acid, and Kaempferol-3, 7-di-O-rhamnoside.
How was cytotoxicity of the compounds tested?
Cytotoxicity was tested using the MTT assay on leukemic cell lines U937, K562, and HL60.
Which compound showed maximum cytotoxicity?
Kaempferol-3, 7-di-O-rhamnoside (OS-4) showed maximum cytotoxicity, followed by Ursolic acid (OS-3).
What further analysis is suggested?
Detailed analysis must be carried out to determine the mode of cell death caused by the drugs.
Glossary definitions and references:
Scientific and Ayurvedic Glossary list for 鈥淎nti leukaemic activity of evolvulus alsinoides鈥�. This list explains important keywords that occur in this article and links it to the glossary for a better understanding of that concept in the context of Ayurveda and other topics.
1) Shirisha (艢ir墨峁, Sir墨sa, 艢ir墨峁, Sirisa, Shirisa, Sirisha):
Sirisha is the first author of the study, affiliated with Karnataka College of Pharmacy in Bangalore. Her research focuses on the isolation and characterization of compounds from Evolvulus alsinoides and their antileukemic activity. Her contact information is included for correspondence regarding the study.
2) Cancer:
Cancer is the central focus of the study, specifically leukemia. The research aims to explore the antileukemic properties of compounds isolated from Evolvulus alsinoides. The study highlights the importance of finding novel plant-derived compounds for anticancer treatments.
3) Activity:
Activity refers to the biological functions tested in the study. It specifically denotes the antileukemic activity of isolated compounds from Evolvulus alsinoides. The research measures this activity against leukemic cell lines using MTT assays to determine cytotoxic effects.
4) Drug:
Drug refers to Evolvulus alsinoides, known in Ayurveda as Shankhpushpi. The plant is explored for its potential as an anticancer agent. Its traditional usage in Ayurveda for various ailments suggests its medicinal value, warranting scientific investigation for modern therapeutic applications.
5) Epilepsy:
Epilepsy is listed among the various conditions Evolvulus alsinoides is traditionally used to treat. This points to the broad spectrum of its medicinal applications in Ayurveda, suggesting the plant's potential neuroprotective and antiepileptic properties.
6) Anxiety:
Anxiety is mentioned as one of the conditions where Evolvulus alsinoides shows effectiveness. This points to its calming and neuroprotective properties, which have been acknowledged in traditional Ayurvedic medicine and now studied scientifically for validation.
7) Burning sensation:
Burning sensation is another condition treated by Evolvulus alsinoides in traditional medicine. Documenting this helps highlight the plant's multipurpose therapeutic uses, which include addressing sensory disturbances and potentially contributing to its overall anti-inflammatory properties.
8) Ayurveda (膧yurveda, Ayus-veda):
Ayurveda is a traditional system of Indian medicine. Evolvulus alsinoides, known as Shankhpushpi, is a reputed drug in Ayurveda. The study aims to validate these ancient medicinal claims with scientific research, focusing on the plant's antileukemic properties.
9) Neurosis:
Neurosis is cited as one of the conditions treatable by Evolvulus alsinoides. This highlights the plant's use in managing psychological and neurological disorders, emphasizing its wide therapeutic range in traditional Ayurvedic practices.
10) Edema (Oedema):
Oedema, or swelling, is listed among the ailments treatable by Evolvulus alsinoides. This implies the plant's potential anti-inflammatory and diuretic properties documented in Ayurvedic medicine, which the study aims to explore scientifically.
11) Purification:
Purification refers to the process of isolating and identifying pure compounds from the plant extracts. This is achieved through chromatographic techniques, ensuring that the biological activity measured is attributable to specific, well-characterized compounds.
12) Discussion:
Discussion is a section in the study where results are interpreted. It elaborates on the cytotoxic effects of the isolated compounds OS-3 and OS-4, comparing their efficacy against leukemic cell lines and determining their potential for further in-depth studies.
13) Toxicity:
Toxicity in this context refers to the cytotoxic effects measured against leukemic cell lines using MTT assays. The study assesses how the compounds from Evolvulus alsinoides affect cell viability, crucial for understanding their therapeutic window and safety profile.
14) Medicine:
Medicine refers to the use of plant-derived extracts or isolated compounds as therapeutic agents. The study contributes to the field of pharmacognosy by investigating the medicinal properties of Evolvulus alsinoides, particularly its potential use in leukemia treatment.
15) Rama (Ram膩, R膩m膩, R膩ma):
Rama appears in the email contact details of one of the corresponding authors, possibly a collaborator in the study. This name alongside Sirisha's email signifies the avenues for direct academic and research-related communication regarding the study.
Other Science Concepts:
Discover the significance of concepts within the article: 鈥�Anti leukaemic activity of evolvulus alsinoides鈥�. Further sources in the context of Science might help you critically compare this page with similair documents:
Evolvulus alsinoides, Snake-bite, Nervine tonic, MTT assay, Pharmacological studies, Chloroform extract, Methanol extract, Petroleum ether extract, Cancer chemoprevention, Cytotoxicity, Plant Extract, Authentic sample, Chemical compound, Ursolic acid, Petroleum ether, Biological studies, Cytotoxicity assessment, Silica Gel, Cell death, Cell line.