Significance of MTT assay
The MTT assay is a colorimetric method used to assess cell viability and cytotoxicity by measuring the metabolic activity of living cells. It operates on the principle that viable cells can reduce the yellow MTT salt to purple formazan crystals, indicating their presence and health. This assay is frequently employed in various studies, including cancer research, to evaluate the effectiveness of treatments on different cell lines, providing a quantitative measure of cell proliferation and metabolic activity.
Synonyms: Cell viability assay, Cell proliferation assay, Colorimetric assay
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The concept of MTT assay in scientific sources
The MTT assay is a colorimetric assay that evaluates cell viability by measuring the reduction of MTT to formazan. It is used to assess metabolic activity and cytotoxic effects, particularly against cancer cells like PC-3.
From: The Malaysian Journal of Medical Sciences
(1) This is a method that was used to determine cell viability, and it involves the reduction of a tetrazolium salt.[1] (2) This is a specific method that was used to assess the cytotoxicity of goniothalamin in the cells, in order to measure its effects.[2] (3) This is a biochemical assay used to measure the cytotoxic activity of the extracts and compounds against the specified cell lines.[3] (4) MTT assay is a method used to measure cell viability and assess the cytotoxicity of the extracts on cell lines.[4] (5) A laboratory test used to determine the cytotoxicity of tamarillo extracts on cancer cell lines by measuring the reduction of MTT to formazan.[5]