Phytochemical screening and TLC of Ocimum basilicum extract
Journal name: World Journal of Pharmaceutical Research
Original article title: Phytochemical screenings and partial charecterization of ocimumbasilicum phenolic extract by tlc
The WJPR includes peer-reviewed publications such as scientific research papers, reports, review articles, company news, thesis reports and case studies in areas of Biology, Pharmaceutical industries and Chemical technology while incorporating ancient fields of knowledge such combining Ayurveda with scientific data.
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Original source:
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R. Prabhavathi, Dr. S. R. Divya and Dr. A. Chandra Mohan
World Journal of Pharmaceutical Research:
(An ISO 9001:2015 Certified International Journal)
Full text available for: Phytochemical screenings and partial charecterization of ocimumbasilicum phenolic extract by tlc
Source type: An International Peer Reviewed Journal for Pharmaceutical and Medical and Scientific Research
Doi: 10.20959/wjpr20176-8663
Copyright (license): WJPR: All rights reserved
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Summary of article contents:
Introduction
The study conducted by Mohan et al. (2017) focuses on the phytochemical analysis and partial characterization of the phenolic extract from the leaves of Ocimum basilicum (commonly known as basil). This investigation aims to identify the bioactive constituents present in the leaves, which are valuable for their medicinal properties. The study emphasizes the significance of such constituents in nutraceuticals and their potential applications within the food industry, as well as the need for further research to explore the fractionation and identification of specific compounds.
Phytochemical Constituents of Ocimum basilicum
One of the most crucial findings of the study is the identification of various bioactive constituents in the aqueous methanol extract of Ocimum basilicum leaves. Preliminary phytochemical analysis revealed the presence of alkaloids, anthraquinones, coumarins, flavonoids, saponins, phlobatannins, tannins, and terpenoids. The study highlights how these compounds were isolated based on their polarity using different solvents. Understanding the diversity of these compounds opens avenues for their application in nutraceuticals and medicinal formulations.
Impact of Polyphenols
Polyphenols, which are secondary metabolites found predominantly in fruits, vegetables, and beverages, play a vital role in health and nutrition. This study illustrates the role of polyphenols as potential scavengers of free radicals, linking their presence in plant material to antioxidant activities. The research indicates that the concentrations and proportions of polyphenols can be influenced by various factors such as environmental conditions and the ripeness of the plants at the time of harvest. The study highlights that increasing polyphenolic content correlates with enhanced antioxidant activity, an essential aspect of health promotion.
Total Phenolic Content and Extraction Efficiency
The examination of total phenolic content within the leaf extract is another essential aspect of the study. Results indicated that the use of 80% methanol as a solvent at temperatures of 60°C yielded the highest concentration of phenolics. The total phenolic content was quantified as catechin equivalents, demonstrating that Ocimum basilicum could be a rich source of these beneficial compounds. The efficient extraction method developed in this study is proposed as a promising approach for maximizing the yield of phenolic compounds from basil leaves, suggesting improvements in its utilization in various applications.
Conclusion
In summary, the research on Ocimum basilicum by Mohan et al. underscores the plant's potential as a source of valuable bioactive compounds, particularly polyphenols, which offer promising antioxidant properties. The study not only provides insights into the phytochemical profile of basil leaves but also emphasizes the importance of further investigation into the potential health benefits and application of these compounds in the nutraceutical and food industries. The findings reinforce the notion that traditional medicinal plants like Ocimum basilicum can be integral to modern pharmacology and nutrition, warranting more research to fully understand their capabilities and applications.
FAQ section (important questions/answers):
What are the main bioactive constituents found in Ocimum basilicum?
The preliminary phytochemical analysis revealed alkaloids, flavonoids, tannins, terpenoids, and other bioactive compounds in Ocimum basilicum leaves.
What method was used to assess total phenolic content in extracts?
The total phenolic content was determined using Folin-Ciocalteu reagent and spectrophotometric analysis.
Which solvent was found to be effective for extracting phenolics?
80% methanol was identified as the best solvent for extracting phenolics from Ocimum basilicum leaves.
What techniques were used for phytochemical screening of extracts?
Phytochemical screening utilized various tests, including Dragendorff’s test for alkaloids and FeCl3 test for tannins.
How was the characterization of phenolic extracts performed?
Partial characterization was conducted using Thin Layer Chromatography (TLC) with a specific solvent system.
What potential uses do the findings suggest for Ocimum basilicum?
The study suggests that the bioactive compounds may have applications in nutraceuticals and the food industry.
Glossary definitions and references:
Scientific and Ayurvedic Glossary list for “Phytochemical screening and TLC of Ocimum basilicum extract�. This list explains important keywords that occur in this article and links it to the glossary for a better understanding of that concept in the context of Ayurveda and other topics.
1) Flavonoid:
Flavonoid refers to a class of polyphenolic compounds found in various fruits, vegetables, and plants. They are known for their antioxidant properties and ability to combat free radicals, which makes them relevant for preventing oxidative stress-related diseases. In the study of 'Ocimum basilicum,' flavonoids were identified, suggesting potential health benefits.
2) Food:
Food is essential for survival and provides the nutrients required for growth, metabolism, and health maintenance. The study explores the role of phytochemicals derived from 'Ocimum basilicum' in food applications. These bioactive compounds can enhance the nutritional profile and functional properties of food, bridging nutrition and health.
3) Phytochemical:
Phytochemicals are bioactive compounds found in plants, contributing to their color, flavor, and disease resistance. They play significant roles in human nutrition and health. The study emphasizes the identification and characterization of phytochemicals in 'Ocimum basilicum,' underlining their importance in food and nutraceutical industries as health-promoting agents.
4) Table:
In scientific literature, tables are used to organize and present data clearly and concisely. The table in the study provides statistical information regarding phytochemical screenings of 'Ocimum basilicum,' offering an accessible format for comparing the presence of various bioactive compounds, which is essential for understanding their potential health benefits.
5) Disease:
Disease refers to a pathological condition affecting an organism, leading to disturbances in normal function. The study underscores the relevance of phytochemicals, such as those found in 'Ocimum basilicum,' in potentially preventing or treating diseases through their antioxidant activities, linking plant compounds to health and disease management.
6) Water:
Water is a fundamental component of biological systems, crucial for sustaining life. In the context of this study, water is used in the extraction processes of phytochemicals from 'Ocimum basilicum.' The solvent plays a vital role in determining the efficiency of extracting various bioactive compounds, affecting their analysis and potential applications.
7) Study (Studying):
Study is a systematic investigation aimed at discovering or interpreting facts about a subject. The research focuses on the phytochemical screening of 'Ocimum basilicum,' detailing the methods, results, and implications for medicinal uses. It contributes to the growing body of knowledge on plant-derived compounds and their applications in health.
8) Wine:
Wine is a fermented beverage made from grapes, known for its polyphenolic content, particularly resveratrol and flavonoids. The study briefly mentions wine as a source of polyphenols, linking its antioxidant properties to those found in 'Ocimum basilicum,' suggesting similar health benefits derived from consuming plant-based compounds.
9) Tamilnadu (Tamil-nadu):
Tamilnadu is a southern state in India known for its rich biodiversity and traditional medicinal practices. The study originates from Chennai, Tamilnadu, highlighting the local use of plants like 'Ocimum basilicum' in traditional medicine. This regional context emphasizes the cultural significance of phytochemical research in this area.
10) Chandra:
Chandra refers to Dr. A. Chandra Mohan, one of the authors of the study. His academic position lends credibility to the research, indicating expertise in biochemistry and chemistry. The involvement of knowledgeable researchers is crucial in conducting scientific investigations and ensuring the reliability of findings presented in the study.
11) Parshad (Parsad):
Parshad refers to a cited researcher whose work appears in the references of the study. His research on protective actions of plant polyphenols against chromatid breaks contributes to understanding the potential therapeutic effects of phytochemicals. Citing relevant works strengthens the research by linking it to existing literature in the field.
12) Chennai:
Chennai is the capital city of Tamilnadu and a center for educational and research institutions. The study is conducted at a college in Chennai, showcasing the city’s role in advancing scientific knowledge about medicinal plants. This urban context highlights the integration of traditional medicine with modern scientific research.
13) Science (Scientific):
Science is a systematic enterprise that builds and organizes knowledge in the form of testable explanations and predictions about the universe. The research study exemplifies the application of scientific methods to explore phytochemicals in 'Ocimum basilicum,' contributing to the understanding of their health benefits through empirical evidence and analysis.
14) Chang:
Chang refers to a researcher cited in the study whose work investigates antioxidant activities and polyphenol contents. This citation underscores the interconnectedness of research within the scientific community, validating the study’s emphasis on the importance of phytochemicals in health. Such references provide a broader context for the findings presented.
15) Jaya:
Jaya refers to Jaya College of Arts and Science, the institution where the study was conducted. The affiliation underscores the educational context of the research, reflecting the importance of academic institutions in facilitating studies on plant-derived compounds, fostering innovation, and contributing to advancements in pharmaceutical and nutritional sciences.
16) Diet:
Diet encompasses the foods and beverages consumed by individuals, significantly impacting health and nutrition. The study discusses the dietary importance of 'Ocimum basilicum' phytochemicals, drawing attention to their potential roles in improving health outcomes. This connection between diet and plant compounds is crucial for promoting wellness through nutrition.
17) Drug:
Drug refers to a substance used to diagnose, cure, treat, or prevent disease. The research indicates the potential application of phytochemicals from 'Ocimum basilicum' in the development of nutraceuticals and therapeutic agents. Understanding plant-derived compounds opens pathways for innovative drug development based on natural resources.
18) Observation:
Observation refers to the act of noting or perceiving phenomena to gain information. In the study, observations related to the presence of phytochemicals were recorded, forming the basis for further analysis. Careful observations are essential for validating results, leading to reliable conclusions about the medicinal properties of the studied plant.
19) Discussion:
Discussion is a critical analysis of study findings that contextualizes them within existing knowledge. In this study, the discussion reflects on the implications of phytochemical presence for health applications, linking the results back to the broader field of research on natural compounds. Discussing findings is crucial for drawing meaningful conclusions.
20) Substance:
Substance refers to a specific kind of matter with uniform properties. The phytochemicals studied in 'Ocimum basilicum' represent various substances contributing to the plant's medicinal properties. Identifying and characterizing these substances enhances the understanding of their biological activities and potential therapeutic applications in health and nutrition.
21) Triphala (Tri-phala):
Triphala is a traditional Ayurvedic formulation composed of three fruits known for their health benefits. Though not directly studied in the research, it exemplifies the importance of plant-based mixtures in traditional medicine. The relevance of phytochemicals in formulations like Triphala corresponds with findings on compounds from 'Ocimum basilicum,' suggesting similar benefits.
22) Activity:
Activity in a scientific context refers to the ability of substances to produce effects. The research highlights the antioxidant activity of phytochemicals extracted from 'Ocimum basilicum.' Understanding the activities of these compounds is vital for assessing their potential therapeutic effects and applications in health and disease prevention.
23) Hunting:
Hunting typically involves searching for or seeking something, which can relate to the quest for discovering new medicinal compounds from plants. In the context of the study, the 'hunting' for bioactive phytochemicals in 'Ocimum basilicum' reflects a broader scientific goal of identifying natural substances with health-promoting properties in herbal medicine.
24) Species:
Species is a taxonomic category that identifies a group of organisms capable of interbreeding. In this study, 'Ocimum basilicum' is the focal species, representing a plant with significant phytochemical content. The understanding of species diversity is crucial for exploring new sources of bioactive compounds with potential health benefits.
25) Harvesting (Harvest):
Harvest refers to the process of gathering mature crops from the fields. In the study of 'Ocimum basilicum,' the timing and method of harvesting can influence the quality and quantity of phytochemicals present. This aspect is crucial for maximizing the extraction of beneficial compounds for further research and applications.
26) Greece:
Greece is referenced in connection with studies on polyphenolic content in regional wines. The mention of Greece highlights the international context of phytochemical research and the shared global interest in understanding the health benefits of plant compounds, connecting findings from 'Ocimum basilicum' with those from other cultural contexts.
27) Powder:
Powder is a fine, dry mist of small solid particles often derived from grinding substances. In the study, the leaves of 'Ocimum basilicum' are ground into a powder for extraction purposes. The powder form increases the surface area, enhancing the extraction efficiency of bioactive compounds, which is vital for subsequent analyses.
28) Divya:
Divya refers to Dr. S. R. Divya, an assistant professor involved in the research. Her contributions lend expertise to the study, enhancing its credibility. The role of academic professionals like Divya is essential for conducting thorough scientific investigations and ensuring rigorous methodologies in exploring medicinal plants' properties.
29) Glass:
Glass refers to a transparent material used for laboratory equipment. In the study, glassware is emphasized for sterilization processes during the extraction of 'Ocimum basilicum' phytochemicals. The proper use of glass apparatus is crucial for maintaining experimental integrity, preventing contamination, and ensuring accurate results in scientific research.
30) Death:
Death is the cessation of all biological functions that sustain a living organism. The mention of death relates to the research on oxidative stress and degenerative diseases, where the study of phytochemicals, like those from 'Ocimum basilicum,' aims to find compounds that may mitigate health risks associated with aging and promote longevity.
31) Wall:
Wall refers to the structure surrounding cells in plants. In the context of the study, plant cell walls can comprise insoluble phenolic compounds. Understanding where these compounds are located in the plant contributes to the overall knowledge of phytochemical distribution, aiding in the extraction and utilization of beneficial substances from plants.
Other Science Concepts:
Discover the significance of concepts within the article: �Phytochemical screening and TLC of Ocimum basilicum extract�. Further sources in the context of Science might help you critically compare this page with similair documents:
Medicinal use, Environmental factor, Phytochemical screening, Thin Layer Chromatography, Reactive oxygen species, Total phenolic content, Solvent system, Polyphenolic compound, Crude methanol extract, Bioactive constituent.