Isolation and evaluation of antibacterial 8-hydroxyl naringenin.
Journal name: World Journal of Pharmaceutical Research
Original article title: Isolation, characterization and evaluation of antibacterial activity of a flavanone derivative 8- hydroxyl naringenin from elephantopus scaber linn
The WJPR includes peer-reviewed publications such as scientific research papers, reports, review articles, company news, thesis reports and case studies in areas of Biology, Pharmaceutical industries and Chemical technology while incorporating ancient fields of knowledge such combining Ayurveda with scientific data.
This page presents a generated summary with additional references; See source (below) for actual content.
Original source:
This page is merely a summary which is automatically generated hence you should visit the source to read the original article which includes the author, publication date, notes and references.
Jyothi Abraham and T. Dennis Thomas
World Journal of Pharmaceutical Research:
(An ISO 9001:2015 Certified International Journal)
Full text available for: Isolation, characterization and evaluation of antibacterial activity of a flavanone derivative 8- hydroxyl naringenin from elephantopus scaber linn
Source type: An International Peer Reviewed Journal for Pharmaceutical and Medical and Scientific Research
Copyright (license): WJPR: All rights reserved
Download the PDF file of the original publication
Summary of article contents:
Introduction
The study focuses on the isolation, characterization, and evaluation of the antibacterial activity of a flavanone derivative, 8-hydroxyl naringenin, derived from the medicinal plant Elephantopus scaber Linn., which belongs to the Asteraceae family. Traditionally, parts of this plant are utilized for treating various ailments including diarrhea, liver disorders, tumors, inflammation, and ulcers. The research highlights the significance of plant-derived medicinal compounds, particularly in light of rising antibiotic resistance, and underscores the urgent need to discover new, effective antimicrobial agents from natural sources.
Isolation and Characterization of 8-Hydroxyl Naringenin
Utilizing bioactivity-guided fractionation of the acetone extract of Elephantopus scaber, researchers successfully isolated a novel flavanone known as 8-hydroxyl naringenin. The characterization of this compound was achieved through a combination of Nuclear Magnetic Resonance (NMR) spectroscopy, mass spectrometry, and Infrared (IR) spectroscopy, confirming its molecular formula as C₁₅H₁₄O�. These evaluations indicated the presence of hydroxy and aromatic groups, crucial for understanding the compound's potential biological activities. The significant structural elucidation provided foundational knowledge for its subsequent pharmacological evaluation.
Antibacterial Activity Assessment
The antibacterial activity of the isolated compound A was rigorously tested using the disc diffusion method against 12 bacterial strains, including both gram-positive and gram-negative species. Notably, the isolated 8-hydroxyl naringenin exhibited a pronounced antibacterial effect with a maximum inhibition zone of 40.5 mm against Salmonella typhi, indicating its potency compared to the crude acetone extract of E. scaber, which displayed minimal antibacterial activity. This stark difference underscores the value of isolating specific compounds from complex mixtures to achieve enhanced therapeutic efficacy.
The Potential of Medicinal Plants
The research reinforces the importance of traditional medicine and the role of indigenous herbs in offering safer alternatives to synthetic drugs, particularly in light of increasing antibiotic resistance. The authors stress that medicinal plants represent a substantial natural resource, contributing to both health care and economic stability in rural populations. By focusing on active compounds such as 8-hydroxyl naringenin, this study exemplifies how phytochemicals can serve as a foundation for modern pharmacological advancements, paving the way for the development of new antimicrobial therapies.
Conclusion
The study successfully identifies and evaluates 8-hydroxyl naringenin as a promising antimicrobial agent isolated from Elephantopus scaber. Given the alarming rise of multidrug-resistant bacterial strains, the findings of this research advocate for further exploration and development of plant-derived compounds in the quest for new antimicrobial drugs. The medicinal potential of E. scaber and similar plants highlights the need for continued interdisciplinary research that bridges traditional knowledge with modern scientific inquiry, ultimately contributing to more effective healthcare solutions.
FAQ section (important questions/answers):
What is the objective of the study on Elephantopus scaber?
The study aimed to isolate, characterize, and evaluate the antibacterial activity of a flavanone derivative named 8-hydroxyl naringenin from the plant Elephantopus scaber.
What are the medicinal uses of Elephantopus scaber?
Elephantopus scaber is traditionally used to treat diarrhea, liver disorders, tumors, inflammations, and ulcers, indicating its significance in traditional medicine.
What methods were used to isolate 8-hydroxyl naringenin?
The isolation involved bioactivity-guided fractionation using acetone extract, followed by silica gel column chromatography and confirmation through NMR and mass spectrometry.
How was the antibacterial activity of the compounds tested?
Antibacterial activity was evaluated using the disc diffusion method against 12 standard bacterial strains, comparing with crude acetone extract and standard antibiotics.
What were the findings regarding the antibacterial activity?
The isolated compound 8-hydroxyl naringenin exhibited significant antibacterial activity against various bacteria, outpacing the antibacterial efficacy of the crude acetone extract.
What is the significance of the study on antibacterial agents?
The study underscores the potential of plant-derived compounds as effective alternatives to synthetic antibiotics, especially in combating multidrug-resistant bacterial strains.
Glossary definitions and references:
Scientific and Ayurvedic Glossary list for “Isolation and evaluation of antibacterial 8-hydroxyl naringenin.�. This list explains important keywords that occur in this article and links it to the glossary for a better understanding of that concept in the context of Ayurveda and other topics.
1) Activity:
Activity in this context refers to the biological effects exhibited by substances, specifically the antibacterial properties of 8-hydroxy naringenin, the flavanone isolated from Elephantopus scaber. This is essential for evaluating the efficacy of medicinal compounds against various bacterial strains, thus contributing to the field of pharmacology and natural medicine.
2) Thomas:
Thomas refers to T. Dennis Thomas, one of the authors in the study. His contributions, especially in the research and development of medicinal plant compounds, are crucial for advancing the understanding of traditional herbal medicine and its applications, particularly in the isolation of bioactive phytochemicals.
3) Antibiotic (Antibacterial):
Antibiotic refers to medicinal compounds used to treat bacterial infections. This term is relevant due to the increasing problem of antibiotic resistance, which the study addresses by exploring plant-derived alternatives, particularly emphasizing the isolated compound's effectiveness compared to conventional antibiotics.
4) Medicine:
Medicine relates to the varied practices and substances used to prevent or treat illnesses. The research focuses on plant-derived medicines, showcasing their role as therapeutic agents and their importance in alternative medicine systems. This underscores the growing interest in natural products for healthcare.
5) India:
India is significant in this study as it serves as the geographical origin for Elephantopus scaber and the research setting. The country has a rich tradition of using medicinal plants in various systems of medicine, which enhances the relevance of this research in the context of local and cultural practices.
6) Palai:
Palai is the location where the research was conducted, specifically at St. Thomas College. This region's rich biodiversity includes many medicinal plants, making it a relevant site for studying the therapeutic potential of local flora in traditional medicine and ethnobotanical applications.
7) Table:
Table in this document summarizes key data, specifically findings related to the antibacterial activity of the isolated compound and the crude extract. It provides a comparative overview of the efficacy against different bacterial strains, allowing quick reference to the study's results and conclusions.
8) Aureus:
Aureus refers to Staphylococcus aureus, a Gram-positive bacterium tested for susceptibility to the isolated compound. Its presence indicates the relevance of the research in studying common pathogens responsible for infections, thus contributing to the understanding of potential treatments derived from natural sources.
9) Powder:
Powder refers to the powdered form of the dried leaves of Elephantopus scaber used for extraction purposes in the study. The process of grinding plant material into powder increases the surface area, facilitating better extraction of bioactive compounds, which is crucial for subsequent analyses.
10) Drug:
Drug signifies any substance used for medical treatment. This research explores the potential of plant-derived substances as drugs, focusing on their isolation and evaluation for pharmacological activity. It highlights the importance of discovering new therapeutic agents from natural sources, particularly as alternatives to synthetic drugs.
11) Botany:
Botany is the scientific study of plants, and it plays a central role in this research. The investigation of medicinal plants like Elephantopus scaber falls within the realm of botany, emphasizing the importance of identifying and understanding plant species with therapeutic potential.
12) Phytochemical:
Phytochemical refers to the bioactive compounds derived from plants. This study isolates and characterizes phytochemicals, specifically flavonoids, contributing to the body of knowledge on their health benefits, including antibacterial properties, and their potential application in developing new natural medicines.
13) Disease:
Disease highlights the focus of the research on medicinal plants as a source for treatment options. The investigation of antibacterial properties is directly linked to combating bacterial diseases, underlining the importance of natural compounds in addressing health issues associated with these pathogens.
14) Surface:
Surface refers to the area of contact where antibacterial activity is assessed, particularly in the disc diffusion assay. This term emphasizes the methodology used in testing the effectiveness of the isolates against bacteria, showcasing the practical applications of the research findings.
15) Kerala:
Kerala is the state in India where the study was conducted and where the Elephantopus scaber plant was sourced. The state's diverse flora contributes significantly to the exploration of traditional medicinal practices, making it an important site for ethnobotanical studies on medicinal plants.
16) Family:
Family refers to the botanical classification of Elephantopus scaber, which belongs to the Asteraceae family. Understanding the family classification aids in identifying the plant's characteristics and its relatives, thus providing insights into the potential uses and benefits of similar species.
17) Medium:
Medium refers to the nutrient agar used in the antibacterial assay to cultivate bacterial strains. The choice of medium is essential for accurate testing, as it influences bacterial growth. This term underscores the methodology's importance in evaluating the antibacterial activity of the extracts.
18) Study (Studying):
Study refers to the research effort undertaken to isolate and evaluate the antibacterial properties of 8-hydroxy naringenin from Elephantopus scaber. It serves as a systematic investigation contributing knowledge regarding the medicinal potential of local plants and their bioactive compounds.
19) Central India:
Central India is relevant as it represents a region where Elephantopus scaber is found. Understanding the geographical distribution of the plant enhances the contextual significance of its medicinal uses within traditional practices of local populations and contributes to the broader field of ethnobotany.
20) Human body:
Human body relates to the target of the medicinal properties investigated in the study. The research focuses on how phytochemicals interact with bacterial pathogens that affect human health, thereby contributing to the understanding of natural treatments and their effects on human physiology.
21) Marudhamalai (Maruthamalai):
Maruthamalai refers to the road where Bharathiar University is located, and it suggests the specific locale of the research institutions involved in the study. Geographical specifics may enhance the understanding of local flora and its traditional uses, thus contextualizing the research further.
22) Purification:
Purification pertains to the process of isolating 8-hydroxy naringenin from the crude extract. It is a critical step in evaluating the compound's efficacy, ensuring that the results reflect the true activity of the isolated substance without interference from other constituents in the extract.
23) Inflammation:
Inflammation is a biological response linked to the medicinal uses of Elephantopus scaber. The study's background highlights traditional uses of the plant in treating inflammatory conditions, thereby connecting historical practices with new scientific findings regarding active compounds with potential therapeutic effects.
24) Surrounding:
Surrounding refers to the area around the test samples during bacterial assays. The interaction between the extract and bacteria is critically assessed in this zone of inhibition, which is vital for determining the effectiveness of the antibacterial activity of the isolated compounds.
25) Observation:
Observation in the study pertains to the careful monitoring of bacterial growth inhibition during experiments. Rigorous observation is essential for accurately measuring the efficacy of antibacterial agents, contributing to the scientific rigor and reliability of the findings presented.
26) Discussion:
Discussion refers to the section of the research where the findings are interpreted and contextualized. It allows researchers to explore the implications of their results concerning existing knowledge, which is critical for advancing understanding in the field of pharmacognosy and traditional medicine.
27) Tamilnadu (Tamil-nadu):
Tamil Nadu is the state in which Bharathiar University is located, emphasizing the geographical context of the research. The state's rich heritage in traditional medicine and vast biodiversity makes it a significant area for studying medicinal plants like Elephantopus scaber.
28) Snake-bite:
Snake-bite highlights one of the traditional uses of Elephantopus scaber as an antidote. Studying plants with ethnomedicinal applications can provide insights into their potential efficacy and further validate their historical significance in treating specific health conditions.
29) Science (Scientific):
Scientific pertains to the methodical approach taken in conducting the research. The use of rigorous scientific procedures and techniques in isolating and evaluating compounds is essential for validating traditional knowledge and ensuring the reliability of the findings in the study.
30) Knowledge:
Knowledge in this context relates to the understanding of medicinal plants and their bioactive properties. The research integrates traditional knowledge with modern scientific inquiry, contributing to the broader discourse on the importance of preserving and utilizing ethnobotanical wisdom.
31) Flavonoid:
Flavonoid represents a class of plant secondary metabolites to which 8-hydroxy naringenin belongs. This term indicates the focus on these compounds in research due to their diverse biological activities, including antibacterial effects, thus highlighting their significance in medicinal plant studies.
32) Species:
Species refers to the specific categorization of Elephantopus scaber within the plant kingdom. The identification of this species as a source of medicinal compounds underscores the importance of biodiversity in natural product research, promoting conservation and sustainable use of medicinal flora.
33) Filling (Filled):
Filling in this text conveys the idea of addressing gaps in knowledge about effective antimicrobial treatment options. The exploration of novel compounds aligns with the urgent need to find alternatives to conventional antibiotics, contributing to the ongoing fight against antibiotic resistance.
34) Purity:
Purity relates to the quality of the isolated compound, indicating the extent to which it is free from impurities. In research, assessing the purity is critical as it affects the evaluation of the bioactivity of the compound, determining its efficacy in antibacterial assays.
35) Nature:
Nature symbolizes the source of medicinal compounds, emphasizing the relationship between natural environments and human health. The study reinforces the concept of utilizing nature's resources to find alternative treatments, highlighting the importance of plant biodiversity in medicine and health care.
36) Dealer:
Dealer refers to the local authorized retailer from whom chromatography materials were sourced. This mention emphasizes the importance of utilizing local resources and suppliers in the research process, thus contributing to the practical aspects of conducting scientific inquiry.
37) Campu:
Campu, likely a misspelling of 'campus', pertains to the setting where the research was conducted, specifically at St. Thomas College. It emphasizes the institutional context and accessibility of resources for students and researchers engaging in botanical and pharmacological studies.
38) Water:
Water refers to a solvent used in the extraction process of bioactive compounds from plant material. It plays a crucial role in ensuring the efficacy of extraction techniques, highlighting its importance in the preparation of extracts for pharmaceutical evaluations.
39) Ulcer:
Ulcer is one of the traditional ailments treated using Elephantopus scaber. Understanding the historical and cultural significance of such traditional uses informs the research on the plant's potential pharmacological benefits, thereby merging traditional practices with contemporary scientific inquiry.
40) Tamil:
Tamil denotes the language spoken in Tamil Nadu, the region where the research was conducted. The inclusion of Tamil highlights the cultural aspect of the research environment, underlining the connection between local languages, traditional medicine, and the contextual significance of the study's findings.
41) Nadu:
Nadu completes the reference to Tamil Nadu, emphasizing the geographical and cultural identity of the region. The significance lies in the understanding of how local culture influences traditional practices regarding medicinal plants and the research efforts to validate and explore these traditions.
42) Road:
Road, specifically referring to Maruthamalai Road, signifies the accessibility of the research institution where the study took place. It provides geographical context, enhancing understanding of the study's environment, connectivity to resources, and the significance of regional flora in medicinal research.
Other Science Concepts:
Discover the significance of concepts within the article: �Isolation and evaluation of antibacterial 8-hydroxyl naringenin.�. Further sources in the context of Science might help you critically compare this page with similair documents:
Medicinal plant, Phytochemical, Treatment of disease, Therapeutic agent, Traditional medicine, Antibacterial activity, Antimicrobial activities, Research article, Disc diffusion method, Zone of inhibition, Elephantopus scaber, Asteraceae family, Clinical observation, Acetone extract, Gram positive bacteria, Gram negative bacteria, Bioactivity-guided fractionation, Elemental analysis, Herbal drug, Spectral data, Mass spectrum, Multidrug-resistant Strains, Biologically active compound, Polyphenolic compound, Inhibitory action, Compound A, Phytochemical constituent.