LC-MS Analysis of Kigelia Pinnata Root Bark: A Medicinal Plant
Journal name: Ayushdhara
Original article title: LC-MS Analysis of Kigelia Pinnata (JACQ) DC. Root Bark- A Multi-Potent Medicinal Plant
AYUSHDHARA is an international peer-reviewed journal. It focuses on research in Ayurveda, Yoga, Unani, Siddha, Homeopathy, Allopathy, and Pharmaceutical Sciences.
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Dabhi Swati
PhD Scholar, Institute of Teaching and Research in Ayurveda, INI, Jamnagar, Gujarat, India
Parkhi Vishwatej
PhD Scholar, Institute of Teaching and Research in Ayurveda, INI, Jamnagar, Gujarat, India
Shukla Vinay
Head of Department, Department of Pharmaceutical Laboratory, Institute of Teaching and Research in Ayurveda, INI, Jamnagar, Gujarat, India
Patel Bhupesh
Head of Department, Department of Dravyaguna, Institute of Teaching and Research in Ayurveda, INI, Jamnagar, Gujarat, India
Ayushdhara:
(A peer-reviewed, bi-monthly open-access journal)
Full text available for: LC-MS Analysis of Kigelia Pinnata (JACQ) DC. Root Bark- A Multi-Potent Medicinal Plant
Year: 2023 | Doi: 10.47070/ayushdhara.v10i2.1195
Copyright (license): CC BY-NC-SA 4.0
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Summary of article contents:
Introduction
Kigelia pinnata (Jacq) DC., a member of the Bignoniaceae family, is a well-regarded medicinal plant known for its diverse therapeutic applications, particularly in traditional African medicine. It is now widely found in India and is utilized in treating a range of health conditions, including gynecological issues, infections, and various biological disorders. The root bark of K. pinnata is notable for its rich profile of phytochemicals that contribute to its medicinal properties, such as antimalarial, antioxidant, and anticancer activities. This study utilized Liquid Chromatography-Mass Spectroscopy (LC-MS) to extensively analyze the bioactive components present in the methanolic extract of K. pinnata root bark, revealing critical information about its phytochemical constituents and their potential therapeutic uses.
Diversity of Bioactive Compounds
The LC-MS analysis identified a total of 63 known compounds in the methanolic extract of K. pinnata, with 27 compounds found in positive mode and 36 in negative mode. Among the identified compounds, terpenoids represented the largest class, indicating a strong potential for anticancer, antimicrobial, and anti-inflammatory properties. Other significant classes included flavonoids, known for their antioxidative and anti-inflammatory effects, and phenols, credited with various health benefits such as antimicrobial and neuroprotective activities. The findings highlight the complexity and diversity of the phytochemical profile in K. pinnata root bark, suggesting a multifaceted approach to understanding its medicinal benefits.
Application of LC-MS Technique
The use of LC-MS technology in this study allowed for a sophisticated analysis of the intricate mixture of compounds present in the root bark extract. The method provided valuable insights into the chemical composition and facilitated the identification of both known and unknown compounds, enhancing the understanding of K. pinnata's pharmacological potential. The study included a detailed examination of the extracted components, comparing them against established databases to ascertain their molecular identities and potential biological activities. This thorough analytical approach underscores the importance of advanced methodologies in phytochemical research and drug development.
Conclusion
The findings from this study elucidate the rich phytochemical landscape of K. pinnata root bark, encompassing a variety of compounds with significant biological activities. The identification of 63 compounds through LC-MS provides substantive evidence supporting the traditional uses of K. pinnata, asserting its value as a therapeutic herb in folk medicine. The results not only demonstrate the plant's pharmacological potential but also pave the way for further research into its various applications for human health. The study validates the importance of preserving traditional medicinal knowledge while integrating modern analytical techniques to explore the benefits of such plants.
FAQ section (important questions/answers):
What is the primary focus of the study on Kigelia pinnata?
The study aims to explore the biological active components in the methanolic root bark extract of Kigelia pinnata using Liquid Chromatography-Mass Spectroscopy (LC-MS). It identifies various phytochemicals responsible for its traditional medicinal properties.
What types of compounds were identified in the study?
A total of 63 compounds were identified, including terpenoids, flavonoids, phenols, glycosides, and others. These compounds exhibit a range of biological activities such as antioxidant, anti-inflammatory, antimicrobial, and anticancer properties.
How was the extract of Kigelia pinnata prepared for analysis?
The root bark was collected, dried, and then coarse powder was prepared. This powder was extracted with methanol overnight to obtain the crude extract for further analysis by LC-MS to identify the phytochemical components.
Glossary definitions and references:
Scientific and Ayurvedic Glossary list for “LC-MS Analysis of Kigelia Pinnata Root Bark: A Medicinal Plant�. This list explains important keywords that occur in this article and links it to the glossary for a better understanding of that concept in the context of Ayurveda and other topics.
1) Disease:
Many diseases can be treated with the medicinal properties of Kigelia pinnata. It has been traditionally used against fibroid, cancer of the uterus, tapeworm infections, gastrointestinal problems, venereal diseases, rheumatism, and more.
2) Beta:
[see source text or glossary: Beta]
3) Drug:
Kigelia pinnata is a reputed drug in African folklore and is now found abundantly in India. Various parts of the plant, particularly the root bark, have applications in traditional medicine to treat a wide range of medical conditions.
4) Flavonoid:
Flavonoids are one of the many classes of compounds identified in the methanolic extract of Kigelia pinnata. These compounds exhibit antioxidative, cardioprotective, antidiabetic, anti-inflammatory, anti-allergic, antiviral, and anticancer properties.
5) Phytochemical:
Phytochemicals are chemicals derived from plants, mainly secondary metabolites. They play a crucial role in drug development for treating human diseases, and the phytochemical profile of Kigelia pinnata root bark was investigated in this study.
6) Medicine:
Plant-based medicines derived from phytochemicals are used widely across the world, particularly in Africa, Asia, and Latin America. Kigelia pinnata is a well-reputed plant in traditional medicine, used to treat multiple ailments.
7) Cancer:
Kigelia pinnata root bark is known for its medicinal properties in treating cancer, particularly cancer of the uterus. It possesses cytotoxic and anticancer activities attributed to its secondary metabolites.
8) Powder:
The root bark of Kigelia pinnata was shade dried and pulverized into coarse powder, which was then stored for further extraction and analysis. This powder is a crucial step in preparing phytochemical extracts.
9) India:
Kigelia pinnata, originally an African folklore plant, is now found abundantly throughout India. The research and collection were specifically conducted in Gujarat, India.
10) Pharmacological:
The study explores the pharmacological activities of Kigelia pinnata, which include antimalarial, antiprotozoal, antioxidant, antibacterial, and antifungal actions, among others. These properties are due to the plant's diverse phytochemical compounds.
11) Gujarat:
The collection and authentication of Kigelia pinnata root bark were carried out in the Jamnagar district of Gujarat state, India. The research was conducted at the Institute of Teaching and Research in Ayurveda, Jamnagar.
12) Family:
Kigelia pinnata belongs to the Bignoniaceae family. This classification is significant for understanding the plant's characteristics and its traditional uses in various medicinal applications.
13) Nature:
[see source text or glossary: Nature]
14) Cutan:
Sudan III is identified as a compound obtained from the methanolic extract of Kigelia pinnata. It belongs to the azo group and serves as a coloring agent.
15) Dravyaguna (Dravya-guna):
Dravyaguna is the Department of the Institute of Teaching and Research in Ayurveda, Jamnagar, where Bhupesh Patel is the head. This department is involved in the study and research of medicinal plants like Kigelia pinnata.
16) Discussion:
[see source text or glossary: Discussion]
17) Knowledge:
[see source text or glossary: Knowledge]
18) Colouring (Coloring):
Sudan III, a compound found in Kigelia pinnata, belongs to the azo group and is used as a coloring agent. It highlights the plant’s potential for diverse applications in various industrial sectors.
19) Ayurveda (Ayus-veda):
Kigelia pinnata’s medicinal properties and phytochemical profile were studied at the Institute of Teaching and Research in Ayurveda, Jamnagar. The plant’s uses align with Ayurvedic practices in treating various diseases.
20) Village:
The root bark of Kigelia pinnata was collected from the Naranpur village area near the Ranjit Sagar dam in the Jamnagar district of Gujarat, India. This collection site was chosen following Good Field collection practices.
21) Mineral:
[see source text or glossary: Mineral]
22) Shukla (Sukla):
Shukla Vinay is the Head of the Department of Pharmaceutical Laboratory at the Institute of Teaching and Research in Ayurveda, Jamnagar, and a co-author of the study on Kigelia pinnata.
23) Insect:
Phytochemicals synthesized by plants, like those found in Kigelia pinnata, include compounds that protect against insect attacks and planting diseases. These properties have medicinal applications.
24) Sagar (Sagár):
The Ranjit Sagar dam is a significant landmark near Naranpur village in Gujarat state, where the root bark of Kigelia pinnata was collected for the study.
25) Patel:
Patel Bhupesh is the Head of the Department of Dravyaguna at the Institute of Teaching and Research in Ayurveda, and a co-author of the study on Kigelia pinnata.
26) Ayus (Ayush):
The study on Kigelia pinnata was published in AYUSHDHARA, an international journal of research in AYUSH (Ayurveda, Yoga, Naturopathy, Unani, Siddha, and Homeopathy) and allied systems.
Other Science Concepts:
Discover the significance of concepts within the article: �LC-MS Analysis of Kigelia Pinnata Root Bark: A Medicinal Plant�. Further sources in the context of Science might help you critically compare this page with similair documents:
Flavonoid, Phytochemical, Medicinal properties, Significant role, Antioxidant activity, Pharmacological activities, Antimicrobial activity, Phytochemical Profile, Secondary metabolite, Retention time, Drug collection, Plant-based medicine, Antimalarial activity, Terpenoid, Anticancer activities, Phenol, Root bark, Bioactive Molecule, Glycoside.