In vitro Antioxidant and Antilipidperoxidative potential of Pleurotus florida
Journal name: Ancient Science of Life
Original article title: In vitro Antioxidant and Antilipidperoxidative potential of Pleurotus florida
The ANSCI is a peer-reviewed, open-access journal focused on Ayurveda and traditional medicines. It publishes original research, reviews, and literary studies linking traditional knowledge with modern science, covering disciplines like botany, ethnomedicine, pharmacology, and clinical research.
This page presents a generated summary with additional references; See source (below) for actual content.
Original source:
This page is merely a summary which is automatically generated hence you should visit the source to read the original article which includes the author, publication date, notes and references.
S. Selvi, P. Chinnaswamy
Ancient Science of Life:
(A quarterly multi-disciplinary scientific research journal in Ayurveda)
Full text available for: In vitro Antioxidant and Antilipidperoxidative potential of Pleurotus florida
Year: 2007
Copyright (license): CC BY-NC-SA
Download the PDF file of the original publication
Summary of article contents:
Introduction
The research focuses on the antioxidant and antilipid peroxidative properties of the ethanolic extract of the edible mushroom Pleurotus florida, commonly known as oyster mushroom. The study emphasizes the significance of lipid peroxidation (LPO) in various diseases and the role of reactive oxygen species (ROS) in mediating oxidative damage to cellular membranes. The extracts were evaluated using in vitro assays like DPPH (1,1-diphenyl-picrylhydrazyl) scavenging activity, Ferric Reducing Antioxidant Power (FRAP) assay, and antilipid peroxidative activity using goat liver homogenates and erythrocyte ghost (RBC membrane) models.
Antioxidant Potential of Pleurotus florida
The findings demonstrate that the ethanolic extract of Pleurotus florida significantly inhibited LPO in both the RBC ghost and goat liver homogenate models, with higher efficacy observed in the RBC membrane model. The antioxidant activity was assessed through the DPPH and FRAP assays, revealing a dose-dependent scavenging property of the mushroom extracts. Specifically, the extract exhibited an IC50 value of 62.05 Âľg for DPPH degradation, indicating strong free radical scavenging capabilities compared to the commonly used synthetic antioxidant Îą-tocopherol (IC50 = 18.2 Âľg). These results suggest that Pleurotus florida could serve as a valuable source of natural antioxidants that may mitigate oxidative stress associated with various pathological conditions.
Conclusion
The study reinforces the potential health benefits of Pleurotus florida as a functional food ingredient with significant antioxidant and antilipid peroxidative properties. Given the capacity of the mushroom extract to inhibit lipid peroxidation and scavenge free radicals effectively, it may play a therapeutic role in combating oxidative damage and related diseases. As the search for natural antioxidants continues to gain traction, the incorporation of Pleurotus florida into dietary practices could offer protective effects against oxidative stress and improve overall health.
FAQ section (important questions/answers):
What is the primary focus of the study on Pleurotus florida?
The study investigates the antioxidant and antilipid peroxidative potential of ethanolic extract from Pleurotus florida, using various in vitro models to assess its ability to scavenge free radicals and inhibit lipid peroxidation.
How was the ethanolic extract of Pleurotus florida prepared?
The mushroom samples were dried, powdered, and then extracted with ethanol using a soxhlet apparatus for 72 hours. The ethanol was evaporated, and the concentrated extract was used for further assays.
What methods were used to evaluate the antioxidant activity?
Antioxidant activity was measured using the DPPH radical scavenging assay and the Ferric Reducing Antioxidant Power (FRAP) assay, which assess the extract's ability to reduce free radicals and improve antioxidant capacity.
What were the findings regarding lipid peroxidation inhibition?
The ethanolic extract of Pleurotus florida significantly inhibited lipid peroxidation in both RBC ghost and goat liver homogenate models, with greater inhibition observed in the RBC membrane model compared to the liver homogenate.
Glossary definitions and references:
Scientific and Ayurvedic Glossary list for âIn vitro Antioxidant and Antilipidperoxidative potential of Pleurotus floridaâ�. This list explains important keywords that occur in this article and links it to the glossary for a better understanding of that concept in the context of Ayurveda and other topics.
1) Activity:
The term 'Activity' refers to the functional capabilities of the evaluated mushroom extract. In this study, it is primarily related to the antioxidant and antilipid peroxidative activities of Pleurotus florida. The activity represents the extract's efficacy in scavenging free radicals and inhibiting lipid peroxidation, essential for preventing oxidative damage in cells.
2) Medicine:
The word 'Medicine' points to the therapeutic use of mushrooms, such as Pleurotus florida, which has potential health benefits. Mushrooms are recognized not only as food but also as sources of medicinal compounds that can be harnessed for enhancing health and combating diseases, illustrating their dual role in nutrition and healthcare.
3) Science (Scientific):
The word 'Scientific' underscores the methodical approach taken in the research. It emphasizes the reliance on empirical evidence and established methods to evaluate the antioxidant properties of Pleurotus florida, ensuring that the findings are trustworthy and reproducible within the scientific community.
4) Antibiotic (Antibacterial):
The term 'Antibiotic' refers to substances that can kill or inhibit bacterial growth. Although the primary focus is on antioxidant activity, this term highlights the potential of Pleurotus florida to address infections and promote health through its medicinal properties, contributing to the multifaceted utility of mushrooms.
5) Discussion:
The word 'Discussion' relates to the section in the research paper where findings are interpreted and contextualized. This part connects the results of the antioxidant assays to the broader implications for human health, elaborating on how Pleurotus florida's properties could contribute to the prevention of diseases linked to oxidative stress.
6) Accumulation (Accumulating, Accumulate):
The term 'Accumulate' pertains to the process by which mushrooms gather or produce bioactive compounds. In the context of this study, it reflects on how Pleurotus florida can assimilate various secondary metabolites, enhancing its antioxidant capacity, which may support its therapeutic potential and health benefits.
7) Substance:
The word 'Substance' indicates the various chemical compounds contained within the ethanolic extract of Pleurotus florida. These substances are crucial for the observed antioxidant and antilipid peroxidative activities, playing a significant role in how the mushroom extract can mitigate oxidative damage in biological systems.
8) Kshama (KᚣamÄ, Kᚣama, KᚣÄma):
The term 'Kshama' likely refers to an author or researcher noted in the references. This inclusion highlights the collaborative nature of scientific research, recognizing contributors who have previously worked on antioxidant and antimicrobial properties of mushrooms, thus enhancing the credibility and foundation of the current study.
9) Cancer:
The word 'Cancer' reflects a major health concern linked to oxidative stress and lipid peroxidation. The study prompts the exploration of natural antioxidants like those found in Pleurotus florida as potential protective agents against cancer, aligning with ongoing research into dietary strategies for cancer prevention.
10) Blood:
The term 'Blood' is relevant in the context of the experiments conducted on goat erythrocyte ghosts and liver homogenates. Analyzing blood-derived samples allows researchers to assess the antioxidant effects of mushroom extracts in a biological fluid, contributing to understanding the extract's impact on cellular health and oxidative damage.
11) Hand:
The word 'Hand' may appear in phrases discussing participation or methods in the research. It signifies the importance of human intervention in conducting experiments and analyses, emphasizing the necessity of careful handling and precise methodologies to achieve valid scientific results in studies investigating biological activities.
12) Salt (Salty):
The term 'Salt' pertains to the context of the study's biochemical assays, often used in creating buffers, including Tris Buffered Saline. Salts facilitate the stability of samples and maintain pH levels, essential for the integrity of biochemical tests assessing the antioxidant properties of Pleurotus florida.
13) Drug:
The word 'Drug' signifies the medicinal potential that derived compounds from Pleurotus florida may hold. Given its noted benefits in reducing oxidative stress, the extract may inspire the development of new therapeutic agents, underscoring the continuous search for natural alternatives in pharmacotherapy.
Other Science Concepts:
Discover the significance of concepts within the article: â�In vitro Antioxidant and Antilipidperoxidative potential of Pleurotus floridaâ�. Further sources in the context of Science might help you critically compare this page with similair documents:
Therapeutic agent, Free radical scavenging, Antioxidant capacity, Ethanolic extract, Dose-dependent manner, Reactive oxygen species (ROS), Ferric reducing antioxidant power (FRAP), Supply chain, Antioxidant Defense Mechanism, Pleurotus florida, In vitro antioxidant, Mushroom extracts, Lipid peroxidation (LPO), Toxic by-products, Bioactive Molecule, Hydrogen atom donation, Erythrocyte ghost.