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Chemical, microbiological and comparative fermentation studies on dasamularishta

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Journal name: Ancient Science of Life
Original article title: Chemical, microbiological and comparative fermentation studies on dasamularishta
The ANSCI is a peer-reviewed, open-access journal focused on Ayurveda and traditional medicines. It publishes original research, reviews, and literary studies linking traditional knowledge with modern science, covering disciplines like botany, ethnomedicine, pharmacology, and clinical research.
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Original source:

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Author:

Muzaffer Alam, K.K.S Dasan, B. Rukmani, K.K. Purushothaman


Ancient Science of Life:

(A quarterly multi-disciplinary scientific research journal in Ayurveda)

Full text available for: Chemical, microbiological and comparative fermentation studies on dasamularishta

Year: 1984

Copyright (license): CC BY-NC-SA


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Summary of article contents:

Introduction

Dasamularista is a medicated wine used in Ayurvedic medicine, prepared through a fermentation process involving various ingredients, including Citraka (Plumbago rosea Linn.). This study aimed to analyze the effects of fermentation conditions on the production of alcohol and the presence of microorganisms in the preparation of Dasamularista. In particular, the research focused on the impact of the container type—earthen pot versus glass vessel—on the fermentation outcomes and the quantification of plumbagin, a significant active component in Citraka that was subjected to purification (Sodhana).

Impact of Container Type on Fermentation

The study's findings highlighted the critical role of container type in influencing the fermentation process. Dasamularista prepared in glass vessels produced a higher amount of alcohol compared to those prepared in earthen pots. Additionally, the chemical analysis showcased differences in specific gravity, solid content, and total sugar between the two preparations, indicating a greater availability of sugar in the glass container setup. The purification process (Sodhana) resulted in a significant reduction of plumbagin content, dropping from 7.30 mg/g to 3.65 mg/g after purification. Microbiological assessments revealed the presence of two yeasts and a specific bacterium, Micrococcus luteus, which was non-alcohol-producing. This suggests that the fermentation environment, influenced by the choice of container, not only affects alcohol yield but also impacts the microbial composition and overall product characteristics.

Conclusion

The results of this study underscore the importance of fermentation conditions in the preparation of Dasamularista, particularly the container type, which can lead to substantial differences in alcohol production and chemical composition. The decrease in plumbagin content during the purification process and the identification of specific microorganisms provide critical insights into the quality and efficacy of Ayurvedic preparations. These findings reinforce the necessity for standardized methods and container selection in the production of traditional medicinal formulations to optimize their therapeutic potential. The authors extend their gratitude for financial support to research institutions, further emphasizing the need for collaboration in the study of traditional medicine practices.

FAQ section (important questions/answers):

What is Dasamularista and its significance in Ayurvedic medicine?

Dasamularista is a medicated wine used in Ayurvedic medicine, known for its therapeutic properties. It undergoes fermentation, which is crucial in enhancing its biological activity and efficacy.

How does 'Sodhana' affect the plumbagin content in Citraka?

'Sodhana' is used to purify Citraka, resulting in a 50% reduction in plumbagin content. The process helps eliminate toxins while retaining beneficial properties, ensuring a safer and more effective ingredient for Dasamularista.

What are the differences observed between fermentation in glass and earthen containers?

Fermentation in glass vessels produced higher alcohol content compared to earthen pots. The glass container also exhibited higher specific gravity and solid content, indicating better fermentation efficiency, likely due to reduced sugar loss.

What microorganisms were identified during the fermentation process?

The fermentation process revealed two yeasts and one bacterium identified as Micrococcus luteus. Notably, Micrococcus luteus is a non-alcohol-producing organism, which may influence the overall fermentation process.

Glossary definitions and references:

Scientific and Ayurvedic Glossary list for “Chemical, microbiological and comparative fermentation studies on dasamularishta�. This list explains important keywords that occur in this article and links it to the glossary for a better understanding of that concept in the context of Ayurveda and other topics.

1) Glass:
Glass vessels were used to prepare Dasamularista, demonstrating improved alcohol production compared to earthen pots. Its impermeable nature potentially alters fermentation conditions. The study highlights a contrast in fermentation outcomes influenced by container materials, establishing an essential parameter in Ayurvedic drug preparation methodologies, revealing insights into process optimization.

2) Citraka:
Citraka, or Plumbago rosea, is a medicinal herb integral to Dasamularista, primarily offering stimulant properties. The study emphasized its plumbagin content before and after 'Sodhana' purification. The reduction of plumbagin levels suggests a biochemical change impacting the overall efficacy and safety of the final preparation, critical in Ayurvedic formulations.

3) Drug:
In this context, 'drug' references Dasamularista, a traditional Ayurvedic medicated wine. The research underscores the chemical and microbial changes observed during fermentation, which highlight critical parameters affecting drug efficacy. Understanding these changes is essential for standardizing and improving Ayurvedic medications' safety and therapeutic potential.

4) Dashamularishta (DaÅ›amÅ«lÄriṣṭa):
Dasamularista is a medicated wine in Ayurveda, prepared through fermentation of several herbs. The study analyzes its production methods, emphasizing the impact of various factors including container types on alcohol yield and microbial presence, thus providing insights crucial for its preparation, quality, and potential health benefits.

5) Shodhana (Åšodhana, Sodhana):
Sodhana is a purification process in Ayurveda aimed at enhancing the safety and efficacy of medicinal herbs. This study demonstrated its effectiveness in reducing plumbagin levels in Citraka, highlighting its significance in altering chemical profiles. Such processes are fundamental to ensuring that Ayurvedic drugs meet therapeutic standards.

6) Sugar:
Sugar content significantly affects fermentation in Dasamularista, impacting alcohol production and the physico-chemical characteristics of the final product. The study indicated higher sugar levels in glass vessel preparations, suggesting the importance of sugar in influencing medicinal properties and efficiency during production, relevant for Ayurvedic formulations.

7) Water:
Water plays a crucial role in the 'Sodhana' purification process and fermentation of Dasamularista. It serves as a solvent for extracting beneficial compounds and facilitating fermentation. The study emphasizes managing water quality and quantity, as it directly influences the fermentation efficiency, microbial activity, and overall drug quality.

8) Purification:
Purification refers to the 'Sodhana' process employed to cleanse raw materials like Citraka before use in formulation. This study illustrated its significance in depleting harmful compounds like plumbagin, emphasizing the necessity of purification in enhancing the safety and efficacy of Ayurvedic drugs, crucial for holistic health.

9) Ayurveda (Ä€yurveda, Ayus-veda):
Ayurveda, a traditional Indian medicinal system, emphasizes holistic health using natural remedies. The study explores the fermentation of Dasamularista, showcasing Ayurvedic principles in practice, such as the importance of ingredient purity and natural processes. Understanding these principles helps inform modern applications and validations of traditional methods.

10) Quality:
Quality in this study relates to the efficacy and safety of Dasamularista. Factors like container type and purification processes are essential determinants of the final product's quality. By analyzing chemical and microbial profiles, the research underlines the importance of maintaining high-quality standards in Ayurvedic medicine preparation.

11) Discussion:
The discussion in the study synthesizes findings on various factors affecting Dasamularista's fermentation processes. It evaluates the implications of container choice, purification, and ingredient composition on the drug's quality. This analytical part is vital for understanding the interplay of manufacturing variables in enhancing efficacy and safety.

12) Medicine:
Medicine in this context refers to the application of Dasamularista as a therapeutic agent in Ayurveda. The study underscores its role in holistic healing, as well as the need for empirical validation of traditional practices through modern scientific methods to enhance credibility and ensure safe practices.

13) Dhataki (DhÄtakÄ«, DhÄtaki):
Dhataki refers to the flowers used in Ayurvedic formulations, which are sensitive to geographical variations in microbial content. Earlier research noted differences influenced by soil and climate on microbial presence, showcasing the significance of source materials quality affecting overall product efficacy and consistency in traditional Ayurvedic formulations.

14) Pippali (Pippalī, Pīppalī, Pippalin):
Pippali is an herb used to enhance the preparation of Ayurvedic formulations. It was mentioned in context with the ghee coating of earthen pots, crucial for altering fermentation conditions and potentially enhancing therapeutic qualities. Understanding its use illustrates the intricate knowledge of herb combinations in traditional medicine.

15) Jaggery:
Jaggery, a traditional unrefined sugar, serves as a fermentable component in Dasamularista preparation. The quality of jaggery may influence the total sugar levels and fermentation outcomes. As the study suggests, variations in jaggery quality can directly impact the efficacy of the final product in Ayurvedic medicine.

16) Science (Scientific):
Science refers to the empirical investigation of processes outlined in the study. The chemical and microbiological analyses not only validate traditional Ayurvedic practices but also contribute to the scientific understanding of fermentation and medicinal properties of herbs, fostering a bridge between ancient wisdom and modern scientific methodologies.

17) Siddha (SiddhÄ):
Siddha is another traditional Indian medicinal system often parallel to Ayurveda. While the study primarily focuses on Ayurveda, understanding Siddha practices can provide complementary insights into herbal formulations and their preparation processes, broadening the context of traditional medicine and enhancing integrative health approaches.

18) Vaidya:
Vaidya signifies a practitioner of Ayurveda. Acknowledging Vaidya S. Usman Ali’s input highlights the importance of expert feedback in refining medicinal preparations. Their traditional knowledge is crucial for ensuring the cultural integrity and practical application of the findings from modern scientific research in Ayurvedic practices.

19) Dasa (DÄsa, DaÅ›Ä, DasÄ, DaÅ›a, DÄÅ›a, DaÅ›an, Dasha, Dashan):
K.K.S. Dasan, one of the contributing authors, represents the collaborative effort in investigating Dasamularista. His role, along with others, showcases the interdisciplinary nature of the study, combining traditional Ayurvedic knowledge with scientific inquiry, necessary for progressing the understanding and applications of traditional medicine.

20) Blood:
Blood is mentioned in the context of the physiological effects of Citraka, which impacts blood pressure and circulatory functions. Understanding these effects is essential in Ayurveda for evaluating the systemic impact of herbal medicines, particularly their influence on vital physiological processes relevant to health and wellness.

21) Sweating (Sweat):
Sweat is referenced in relation to Citraka's stimulant effects on bodily functions. The herb's influence on sweat and overall excretion is significant in Ayurvedic pharmacology, as it indicates a need for holistic assessments of herbal preparations, encompassing all bodily functions and their interactions with the environment.

22) Ghi (Ghī, Ghee):
Ghee is clarified butter used here to coat earthen pots, aiding in fermentation and preserving the drug's properties. Its inclusion indicates an understanding of how such traditional ingredients contribute to the overall preparation process, intertwining culinary practices with medicinal applications in Ayurvedic formulations.

23) Shand (Åšaṇá¸, Sand):
Sand is utilized in the fermentation process of Dasamularista, providing a thermal insulation environment for the earthen pot. This method emphasizes the traditional techniques used in Ayurveda, where natural materials enhance medication effectiveness, showcasing the intersection of environmental factors and medicinal outcomes in traditional practices.

Other Science Concepts:

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Discover the significance of concepts within the article: �Chemical, microbiological and comparative fermentation studies on dasamularishta�. Further sources in the context of Science might help you critically compare this page with similair documents:

Fermentation process, Literature survey, Thin Layer Chromatography, Chemical studies, Total sugar content, Plumbagin content, Sodhana purification, Microbiological study, Micrococcus luteus, Alcohol production, Research Acknowledgement.

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