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Significance of PCR

Polymerase Chain Reaction (PCR) is a crucial laboratory technique widely used for amplifying specific DNA sequences. This method has applications in various studies, including the analysis of glucose transporters, genotyping of the MMP-9 gene related to stroke, and the identification of pathogenic bacteria. PCR is employed in different contexts, such as amplifying DNA from infected cells and detecting virulence factors in E. coli isolates. Its high sensitivity and specificity make it a revolutionary tool in molecular biology and clinical diagnostics.

Synonyms: Polymerase chain reaction, Dna amplification, Nucleic acid amplification, Molecular cloning, Genetic testing, Molecular diagnostics, Gene testing, Nucleic acid amplification.

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The concept of PCR in scientific sources

Science Books

PCR, or Polymerase Chain Reaction, is a vital technique for amplifying DNA sequences, enabling the measurement of gene expression and facilitating molecular characterization, such as detecting specific genes like the cfr gene in MRSA isolates.

Significance in Scientific journals, articles, etc.:

From: The Malaysian Journal of Medical Sciences

(1) This is the polymerase chain reaction, which permits potentially unlimited amplification of minute traces of DNA and is currently used for STR typing.[1] (2) This was performed using a thermal cycler system with gradient option, and the conditions and reagent concentrations were adapted from a previous study.[2] (3) This is a molecular technique that has been developed for detecting pathogenic bacteria from the genus Leptospira, providing a sensitive, specific, and rapid method for detecting various microorganisms of clinical importance.[3] (4) A method where DNA microsatellites are amplified using polymerase chain reaction, employing specific primers tagged with fluorescence to yield products of a specific length, crucial for detecting genetic markers.[4] (5) Polymerase chain reaction, a method used to amplify DNA fragments, used to amplify the TSA gene from genomic DNA.[5]

This section includes scientific research in the field of healthcare, often involving traditional medicine systems such as Ayurveda, Unani, Siddha and Holistic health. It includes papers from scientific journals classified as either original, clinical or experimental research, review articles or case reports.