Significance of Nucleic acid amplification
Nucleic acid amplification, particularly through PCR, is a diagnostic technique utilized for analyzing cerebrospinal fluid in cases of tuberculous meningitis (TBM). While this method is highly specific, its sensitivity may not always be optimal. This limitation can result in challenges when attempting to definitively rule out TBM based on negative test results, emphasizing the need for caution in the interpretation of PCR findings in this context.
Synonyms: Dna amplification, Pcr, Polymerase chain reaction, Gene amplification
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The concept of Nucleic acid amplification in scientific sources
Nucleic acid amplification, particularly PCR, is a diagnostic tool for analyzing cerebrospinal fluid in TBM cases. It offers high specificity, although its sensitivity issues may hinder conclusive negative results for this condition.
From: The Malaysian Journal of Medical Sciences
(1) Isothermal nucleic acid amplification is a one-tube and fixed temperature method for amplifying nucleic acids and is a cost-effective substitute for the diagnosis of certain diseases.[1] (2) This is a diagnostic method, specifically PCR, used for cerebrospinal fluid analysis in the case of TBM, is highly specific, but its sensitivity might be suboptimal, potentially preventing a definitive ruling out of TBM with negative results.[2]