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Evaluation of wound healing effect of maerua oblongifolia in albino rats

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Journal name: World Journal of Pharmaceutical Research
Original article title: Evaluation of wound healing effect of maerua oblongifolia in albino rats
The WJPR includes peer-reviewed publications such as scientific research papers, reports, review articles, company news, thesis reports and case studies in areas of Biology, Pharmaceutical industries and Chemical technology while incorporating ancient fields of knowledge such combining Ayurveda with scientific data.
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Original source:

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Author:

N. Arulanand Raj, Dr. V. Gopal, S. Dhivya and Dr. G. Jayabalan


World Journal of Pharmaceutical Research:

(An ISO 9001:2015 Certified International Journal)

Full text available for: Evaluation of wound healing effect of maerua oblongifolia in albino rats

Source type: An International Peer Reviewed Journal for Pharmaceutical and Medical and Scientific Research

Doi: 10.20959/wjpr20191-13812

Copyright (license): WJPR: All rights reserved


Download the PDF file of the original publication


Summary of article contents:

Introduction

The study investigates the wound healing effects of the herbal plant Maerua oblongifolia in albino rats. Wound healing involves a complex process characterized by a sequence of events that aim to restore the integrity of damaged tissue. Traditional remedies have gained popularity as potential alternatives to synthetic medications. Here, the in-vivo efficacy of Maerua oblongifolia extract is compared against the standard antibiotic Soframycin in promoting wound healing.

Mechanism of Wound Healing

Wound healing encompasses several phases, including hemostasis, inflammation, proliferation, and remodeling. The process kicks off with platelet aggregation and the release of clotting factors, leading to the formation of a fibrin clot, which acts as a matrix for subsequent healing processes. Inflammation follows, bringing in immune cells and cytokines to facilitate tissue repair and remodeling. In this study, the focus is on how Maerua oblongifolia extracts may enhance these natural healing pathways through various mechanisms, potentially making it a valuable herbal alternative.

Preparation and Study Methodology

The extraction of Maerua oblongifolia was performed using 70% ethanol for 72 hours, after which the extract was concentrated and dried. The excision wound model involved 18 healthy albino rats, divided into three groups: a control group, a positive control treated with Soframycin, and a test group treated with the herbal extract. Wounds were inflicted on rats, and healing was assessed through wound contraction measurements at specified intervals over 15 days.

Results and Effects of Treatment

The results indicated that the application of Maerua oblongifolia extract significantly promoted wound healing, achieving complete wound coverage in 15 days, comparable to the effects of Soframycin. The study recorded parameters such as wound contraction and epithelialization, confirming the herbal treatment's effectiveness. The wound healing mechanisms were attributed to its ability to form a protective barrier, stimulate fibroblast and capillary formation, and reduce inflammation by scavenging free radicals.

Conclusion

The findings of this study support the potential of Maerua oblongifolia as an effective herbal agent for enhancing wound healing. Its performance in comparison to the standard Soframycin suggests that it could serve as a natural remedy for skin wounds and ulcers, presenting no adverse effects. This reinforces the importance of researching herbal options for treating various ailments, particularly those related to wound management.

FAQ section (important questions/answers):

What was the objective of the study on Maerua oblongifolia?

The study aimed to evaluate the in-vivo wound healing effect of Maerua oblongifolia in albino rats, comparing its effectiveness to the standard treatment, Soframycin.

How was the Maerua oblongifolia extract prepared for the study?

The extract was prepared by soaking 30g of powdered Maerua oblongifolia in 70% ethanol using a Soxhlet extractor for 72 hours, then concentrating the extract using a rotary evaporator.

What model was used to assess wound healing in rats?

An excision wound model was employed, where wounds of 30mm were created on the dorsal thoracic area of the rats, which were then treated with the extract or a standard ointment.

How was wound contraction measured in the study?

Wound contraction was assessed every two days by tracing the raw wound area on transparent paper and calculating the percentage of wound closure based on initial wound size.

What were the main findings regarding wound healing activity?

The research found that the Maerua oblongifolia extract significantly enhanced wound healing, with complete healing observed in 15 days, comparable to the effects of Soframycin.

What key processes contribute to wound healing according to the study?

Wound healing involves several phases such as granulation, collagen formation, and scar maturation, supported by factors like fibroblast activity, cytokines, and the matrix laid down by blood clotting.

Glossary definitions and references:

Scientific and Ayurvedic Glossary list for “Evaluation of wound healing effect of maerua oblongifolia in albino rats�. This list explains important keywords that occur in this article and links it to the glossary for a better understanding of that concept in the context of Ayurveda and other topics.

1) Animal:
The term animal typically refers to multicellular organisms of the kingdom Animalia. In this research, the specific reference to animal is associated with the use of albino rats as essential subjects for conducting controlled experiments to assess the efficacy of herbal extracts on wound healing processes.

2) Activity:
Activity in this study relates to the biological responses and processes involved in wound healing. By measuring wound contraction and closure, the research assesses how the herbal extract influences these activities, thereby evaluating its potential therapeutic benefits in enhancing healing in injured tissues.

3) Study (Studying):
This study investigates the wound healing effects of Maerua oblongifolia in albino rats. It encompasses research design, methodology, and analysis of results, aiming to contribute to existing knowledge in pharmacology and herbal medicine, potentially leading to alternative treatments for skin injuries.

4) Murva:
Murva is the name used for the plant Maerua oblongifolia, known for its medicinal properties. The research focuses on extracting healing compounds from this plant to evaluate its effectiveness in wound healing, highlighting the plant's significance in herbal medicine and potential advantages over synthetic alternatives.

5) Repair:
Repair in the context of wound healing refers to the biological processes that facilitate tissue regeneration and restoration after injury. The study aims to explore how Maerua oblongifolia contributes to effective repair mechanisms, offering insights into enhancing healing through natural resources.

6) Alvar (Arvar, Alwar):
Alwar is a city in Rajasthan, India, where part of the research was conducted at Alwar College of Pharmacy. The location plays a role in the cultural and traditional use of local medicinal plants, and it emphasizes the regional relevance of herbal medicines like Maerua oblongifolia.

7) Rajasthan:
Rajasthan is a state in northwestern India. The research, conducted in this region, underscores the traditional knowledge and usage of local flora for medicinal purposes. The study reflects on the significance of indigenous plants in treating ailments common in this geographic area.

8) Disease:
The term disease refers to a specific pathological condition characterized by a specific set of symptoms and signs. In the context of the study, it emphasizes the potential of herbal extracts to address particular health issues, drawing attention to their therapeutic roles.

9) Water:
Water is crucial in the context of this study, particularly in animal welfare and health. It ensures proper hydration of the albino rats during the experiment, maintaining standard laboratory conditions essential for ethical research practices and the integrity of experimental results.

10) Table:
Tables in the document present quantitative data related to the wound healing effects of Maerua oblongifolia. They illustrate findings like wound size measurements and percentage contractions, enabling easy comparison and clear interpretation of the experimental results, which is vital for scientific reporting.

11) Food:
Food is relevant to the well-being of the experimental animals, ensuring that the albino rats in the study maintained a regular diet. Adequate nourishment is essential for their health and vitality, impacting the outcomes of the wound healing experiments by maintaining normal physiological functions.

12) Measurement:
Measurement is an integral part of the study, as it quantifies healing rates and other parameters to assess the efficacy of the herbal extract. Accurate measurement techniques ensure reliable results, allowing for meaningful comparisons between treatment groups and a thorough understanding of wound healing processes.

13) Puducherry:
Puducherry is a union territory in India where part of the research was related, specifically at Mother Theresa Post Graduate and Research Institute of Health Sciences. Its mention emphasizes the geographical relevance of the study, indicating a regional focus on indigenous medicinal applications.

14) Discussion:
Discussion in research allows for interpreting results and contextualizing findings within the existing body of knowledge. The discussion section explores the efficacy of Maerua oblongifolia, potential mechanisms behind its action, and implications for future research, reinforcing the study's contribution to science.

15) Epilepsy:
Epilepsy is one of the diseases historically treated with Maerua oblongifolia, emphasizing the plant's traditional therapeutic applications. The study's mention of epilepsy highlights the broader potential uses of the plant beyond just wound healing, illustrating its importance in holistic medicinal practices.

16) Science (Scientific):
Science represents the knowledge and methodology employed in conducting this research. It emphasizes the systematic approach taken to investigate the properties of Maerua oblongifolia, reflecting scientific principles used to understand and validate the findings related to wound healing.

17) Species:
Species refers to the specific group of organisms studied, in this case, albino rats, to evaluate the herb's therapeutic effects. The choice of species is crucial for translating findings into possible applications for human medicine and pharmacology, highlighting ecological and biological nuances.

18) Family:
In botanical taxonomy, family refers to a category that groups related plants sharing common traits. Maerua oblongifolia belongs to the Capparaceae family, highlighting its connection to other medicinal plants and underscoring the importance of this biological classification in pharmacological studies.

19) Powder:
Powder refers to the processed form of the plant used for extraction in the study. The preparation of the plant powder is essential for creating the herbal extract, playing a critical role in ensuring effective delivery of the medicinal properties during wound healing experiments.

20) Cotton:
Cotton is likely referenced as part of the materials used in the surgical procedure, particularly for cleaning wounds. Its significance lies in its absorbent properties, which facilitate proper hygiene and disinfection, reducing the risk of infection during the experimental treatment processes.

21) Ulcer:
An ulcer, as a specific form of damage to tissue, draws attention to the potential applications of Maerua oblongifolia beyond standard wounds. The research's implications suggest that the herb could address ulcers, expanding its therapeutic relevance in dermatological medicine.

22) Fever:
Fever is one of the diseases historically treated with Maerua oblongifolia. Its inclusion illustrates the traditional medicinal uses of the plant, emphasizing its relevance in addressing a variety of health conditions alongside its wound healing capabilities.

23) Hair:
Hair, referenced in the context of preparing the albino rats for wound creation, focuses on the necessity of removing fur to facilitate clear visibility and access to the skin. This preparation step is integral to maintaining the integrity of the wound healing assessment.

24) Cage:
The term cage refers to the individual enclosures used for animal housing during the study. Providing an appropriate cage setting is essential for ethical research, ensuring the health and safety of the experimental animals, which contributes to reliable and reproducible results.

25) Rich (Rch):
Rich, referring to A. Rich, may denote the taxonomist who classified Maerua oblongifolia. This indicates the scholarly lineage of the plant, adding to its recognition in botany and as a potential source of medicinal compounds, showcasing its importance in herbal studies.

26) Wall:
Wall likely refers to the structural components of cells or tissues involved in wound healing. The study may imply the interaction between the herbal extract and cellular walls, essential for understanding how Maerua oblongifolia contributes to healing processes at a cellular level.

27) Post:
Post indicates the position or role assigned to the research within the broader scientific discourse or academia. It underscores the significance of the study's findings and implications for future research, aligning with ongoing efforts to explore herbal remedies in pharmacology.

28) Drug:
Drug refers to a substance used for medical purposes, in this case, the herbal extract from Maerua oblongifolia evaluated for its healing properties. It highlights the potential of natural products as alternatives or complements to conventional pharmaceutical treatments, broadening options in wound care.

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Discover the significance of concepts within the article: �Evaluation of wound healing effect of maerua oblongifolia in albino rats�. Further sources in the context of Science might help you critically compare this page with similair documents:

Control group, Wound contraction, Standard group, Ethanol extract, Herbal extract, Collagen deposition, Wound healing activities, Excision wound model, Wound Healing Effect, Soframycin ointment, Healthy albino rats, Albino rat.

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