Journal name: World Journal of Pharmaceutical Research
Original article title: Butachlor induced oxidative and hormonal imbalance correlated with ovarian anomalies in fresh water indian cat fish clarias batrachus (linn.)
The WJPR includes peer-reviewed publications such as scientific research papers, reports, review articles, company news, thesis reports and case studies in areas of Biology, Pharmaceutical industries and Chemical technology while incorporating ancient fields of knowledge such combining Ayurveda with scientific data.
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Subtitle: sem study
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Prakriti Verma and G. B. Chand
World Journal of Pharmaceutical Research:
(An ISO 9001:2015 Certified International Journal)
Full text available for: Butachlor induced oxidative and hormonal imbalance correlated with ovarian anomalies in fresh water indian cat fish clarias batrachus (linn.)
Source type: An International Peer Reviewed Journal for Pharmaceutical and Medical and Scientific Research
Copyright (license): WJPR: All rights reserved
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Summary of article contents:
Introduction
This study investigates the effects of Butachlor, an organochlorine pesticide widely used in Indian agriculture, on the freshwater Indian catfish, Clarias batrachus. The research focuses on the oxidative and hormonal imbalances induced by Butachlor exposure and their correlation with ovarian anomalies. Various physiological parameters, including serum lipid peroxides (LPO), reduced glutathione (GSH), thyroid-stimulating hormone (TSH), and estradiol levels, were measured following treatment with Butachlor over different time intervals. The findings reveal significant alterations in biochemical markers and structural anomalies in the ovarian tissues of the fish, indicating potential reproductive toxicity.
Oxidative Stress Induced by Butachlor
One of the critical findings of the study is the significant increase in serum lipid peroxides (LPO) levels following Butachlor exposure. The results showed a sharp rise in LPO, particularly in the groups treated for 10 and 15 days, indicating extensive oxidative damage to the fish. LPO serves as a marker for oxidative stress and reflects the extent of lipid damage caused by free radicals and reactive oxygen species. The increase in LPO corresponds with a significant decline in reduced glutathione (GSH) levels, which are essential for the antioxidant defense mechanisms in the fish. Thus, Butachlor exposure leads to an imbalance in oxidative processes, affirming its toxicological impact.
Hormonal Disruption in Fish
The study also highlights the hormonal disruption caused by Butachlor, with marked increases in serum TSH levels across all experimental groups. The data showed a consistent increase in TSH, suggesting that Butachlor enhances the thyroid axis� activity, potentially leading to impaired reproductive function in fish. Additionally, there was a corresponding increase in serum estradiol levels, particularly in the group exposed to Butachlor for 5 days. The altered levels of these hormones indicate that Butachlor disrupts normal endocrine function, which could adversely affect ovarian health and development.
Structural Anomalies in Ovarian Tissues
The examination of ovarian tissues through scanning electron microscopy revealed significant morphological changes due to Butachlor exposure. Control group fish displayed normal ovarian structures, while those treated with Butachlor exhibited deformities, such as grooves, pits, and raised surfaces on oocytes, indicating cellular damage. With increasing duration of exposure, the structural integrity of the ovarian cells deteriorated, manifested by severed plasma membranes and loosening of follicular epithelium. These observations suggest that oxidative and hormonal imbalances are closely linked to the observed changes in ovarian architecture, leading to potential fertility problems.
Conclusion
In conclusion, the study demonstrates that Butachlor exposure induces significant oxidative stress and hormonal disruptions in freshwater Indian catfish, Clarias batrachus. The resulting increase in lipid peroxidation and alterations in hormonal levels correlate with structural changes in the ovarian tissues, indicating a detrimental impact on reproductive health. These findings underscore the need for further research into the effects of agricultural chemicals on aquatic life and highlight the importance of assessing the ecological impacts of pesticide use, particularly in relation to fish reproduction and sustainability.
FAQ section (important questions/answers):
What is the impact of Butachlor on Clarias batrachus?
Butachlor exposure induces oxidative stress and hormonal imbalance in Clarias batrachus, leading to gonadal atrophy and ovarian anomalies.
How were the fish exposed to Butachlor in the study?
The fish were exposed to Butachlor at a dose of 1.5µl/L for 5, 10, and 15 days in controlled laboratory conditions.
What were the main biochemical parameters measured in the study?
The study measured serum Lipid peroxides (LPO), reduced glutathione (GSH), Thyroid stimulating hormone (TSH), and Estradiol levels.
What significant changes occurred in serum LPO and GSH levels?
There was a significant increase in LPO levels and a marked decrease in GSH levels in Butachlor-treated fish compared to control groups.
What effects of Butachlor exposure were observed on ovarian structure?
SEM studies revealed that Butachlor exposure caused grooves, papillae, and protuberances on the surface of oocytes, indicating damage to ovarian cells.
What was the conclusion regarding hormonal changes due to Butachlor?
The study concluded that Butachlor exposure causes significant hormonal changes, including increased TSH and Estradiol levels, affecting ovarian dynamics in female fish.
Glossary definitions and references:
Scientific and Ayurvedic Glossary list for “Butachlor causes oxidative, hormonal imbalances in Clarias batrachus.�. This list explains important keywords that occur in this article and links it to the glossary for a better understanding of that concept in the context of Ayurveda and other topics.
1) Fish:
Fish are aquatic vertebrates that are often studied in biochemical and ecological research. In the context of this study, the freshwater Indian catfish, Clarias batrachus, is used to investigate the effects of the pesticide butachlor, particularly its hormonal and oxidative impacts on reproductive health.
2) Surface:
The surface refers to the outer layer or boundary of an object. In the study, the surface of ovarian cells is observed using scanning electron microscopy (SEM) to identify changes caused by butachlor exposure, such as deformities and structures that affect reproductive potential.
3) Pesticide:
Pesticides are chemical substances used to kill pests, including insects and weeds. Butachlor, a specific pesticide investigated in this study, is known to adversely affect aquatic life, leading to hormonal imbalances and changes in the metabolic processes of fish, particularly affecting their reproductive systems.
4) Blood:
Blood is a vital fluid in animals that transports nutrients, gases, and hormones. In the study, blood samples from the fish are analyzed to measure various biochemical parameters, such as lipid peroxides and hormonal concentrations, helping assess the physiological impact of butachlor exposure.
5) Study (Studying):
The study refers to the systematic investigation presented in the text to explore the effects of butachlor on the freshwater Indian catfish. It aims to elucidate the connections between oxidative stress, hormonal changes, and reproductive health based on biochemical and histological findings.
6) Depression:
Depression in this context describes the morphological changes in ovarian cells. In the SEM observations, the surface membrane shows grooves and indentations, indicating possible cellular damage and dysfunction, which is a key outcome of butachlor exposure affecting fish reproductive capabilities.
7) Water:
Water is the essential medium for aquatic life, providing necessary habitat and resources for organisms. In this study, the quality and contamination levels of water are crucial since the freshwater Indian catfish is exposed to a pesticide-laden environment to observe resultant physiological changes.
8) Patna:
Patna is the capital city of Bihar, India, and serves as the location for the research conducted by the authors. The Department of Zoology at Patna University is where the study takes place, emphasizing the regional focus of the investigation on local fish species.
9) Table:
Tables in research documents organize and present data clearly. In this study, various tables summarize the effects of butachlor on the biochemical parameters of the fish, facilitating easy comparison of results across different treatment groups for lipid peroxides, glutathione, TSH, and estradiol levels.
10) New Delhi:
New Delhi, the capital city of India, is mentioned as the location for advanced scientific analysis using facilities like the scanning electron microscope. This highlights the collaboration between different institutions and emphasizes the modern techniques employed in studying the effects of pesticides on fish.
11) Observation:
Observation refers to the systematic recording of phenomena as they occur during a study. In this research, the observations made during SEM evaluations and biochemical analyses are critical for understanding the broader implications of butachlor exposure on the health and reproductive systems of fish.
12) Delhi:
Delhi refers to the national capital territory of India, inclusive of New Delhi. It is significant in the study mainly for its role as a location for advanced research facilities, contributing to the overall scientific inquiry conducted by the authors related to environmental impacts on aquatic life.
13) India:
India is the country where the study is conducted, emphasizing the regional implications of pesticide use in agricultural practices. The focus on Indian freshwater catfish underlines the relevance of local fish species to ecological and environmental health within the context of agricultural pollution.
14) Chand:
Chand is one of the co-authors of the study alongside Prakriti Verma. Their collaboration at the Department of Zoology in Patna University signifies the combined expertise in conducting critical research on the effects of environmental stressors like butachlor on fish biology.
15) Zoology:
Zoology is the scientific study of animals, encompassing their biology, behavior, and ecology. This study falls under zoological research as it examines the physiological and hormonal impacts of a pesticide on a specific fish species, contributing to the broader understanding of environmental toxicology.
16) Prakriti (Prakrti):
Prakriti Verma is the lead author of the study, representing a significant contributor to the research on butachlor effects on the freshwater Indian catfish. Her work highlights the importance of individual researchers in conducting impactful scientific inquiries within the field of zoology.
17) Fixation:
Fixation is a crucial step in preparing biological samples for microscopic examination. In this study, ovarian tissues were fixed in gluteraldehyde, preserving their structure for scanning electron microscopy to assess the morphological alterations induced by butachlor exposure on the fish's reproductive organs.
18) Science (Scientific):
Science is the systematic enterprise that builds and organizes knowledge through testable explanations and predictions regarding the universe. This study exemplifies the scientific method by employing controlled experiments, detailed observations, and statistical analyses to explore the toxic effects of pesticides on fish.
19) Indian:
Indian refers to the geographic and cultural context in which the study is conducted, specifically focusing on the freshwater Indian catfish. Understanding local species and their responses to environmental pollutants is crucial for developing targeted conservation and management strategies within India’s diverse ecosystems.
20) Kumar:
Kumar refers to a co-author or contributing researcher in the study. His presence indicates a collaborative effort in the scientific investigation, which is essential for obtaining a comprehensive understanding of the complex interactions between environmental toxins and aquatic organisms' biological systems.
21) Bihar:
Bihar is a state in India, where the research was conducted. Its geographical and environmental characteristics provide context for the study as it explores the consequences of agricultural practices and pesticide use on local fish populations within the freshwater ecosystems of Bihar.
22) Glass:
Glass refers to the material used to make laboratory equipment, such as the culture tubes and apparatus employed during the blood and tissue collection processes. In scientific experimentation, glassware is critical for ensuring accuracy, sterility, and reliability of chemical reactions and analyses.
23) Field:
Field refers to the agricultural landscape where pesticides like butachlor are commonly used. Understanding the field applications and subsequent runoff into aquatic environments is essential for assessing the broader ecological impact of agricultural practices on the health of local fish species.
24) Miṇi (Mini):
Mini likely refers to specific laboratory equipment used in biochemical assays, such as mini ELISA kits for hormone testing. The miniaturization of equipment allows for efficient use of reagents and smaller sample volumes, streamlining the analysis process in laboratory settings.
25) Rana:
Rana is likely mentioned as a cited author within the text, contributing to the scientific knowledge surrounding pesticide effects on aquatic organisms. His work, along with others, forms part of the information foundation that informs the current investigation into butachlor's impact.
26) Gold (Golden):
Gold is referenced in the context of using sputtering technology for sample preparation prior to SEM analysis. Gold coating enhances the conductivity of biological samples for electron microscopy, allowing for clear visualization of structural details, which is critical for assessing cellular alterations.
27) Post:
Post refers to the stage following initial specimen preparation, particularly in histological processes such as post-fixation. Proper post-fixation treatment is vital for preserving tissue morphology and ensuring accurate observation of structural changes due to exposure to toxic substances.
28) Discussion:
Discussion within a research article provides interpretation and analysis of the results obtained from the study. It allows authors to relate their findings to existing literature, hypothesize implications, and suggest further research directions, emphasizing the significance of their contributions to the field.
29) Developing:
Developing refers to the growth and maturation process of ovarian follicles, which is a focus of the study. Understanding how environmental stressors like butachlor affect developing oocytes is essential for assessing the reproductive health and ecological viability of fish populations.
30) Substance:
Substance typically refers to any form of matter, in this case, the biochemical compounds or pollutants like butachlor being studied. Understanding the impacts of specific substances on aquatic life is important for environmental health assessments and developing effective regulatory measures.
31) Toxicity:
Toxicity denotes the degree to which a substance can harm organisms. This study investigates the toxicity of butachlor on the freshwater catfish, analyzing biochemical and physiological systems to determine how exposure impacts fish health and reproductive capabilities.
32) Activity:
Activity in the context of this research may refer to biological activity, such as hormonal activity and antioxidant defenses. The study measures various biochemical activities to assess the effects of butachlor on fish health, focusing on hormonal balances and oxidative stress responses.
33) Lucknow:
Lucknow is the city in India where SEGNOTECH Agro Pvt. Ltd., the manufacturer of butachlor, is located. This highlights the local production context of the pesticide studied in the research, which is important for understanding its environmental distribution and regulatory implications.
34) Disease:
Disease refers to any condition that negatively affects the health of organisms. The findings of this study can help in understanding how chemical exposures like butachlor can contribute to diseases in fish populations, ultimately impacting biodiversity and ecosystem health.
35) Species:
Species refers to a group of organisms that can interbreed. In this study, Clarias batrachus (Indian catfish) is the primary species under investigation, and understanding its response to environmental stressors is crucial for conservation and management efforts in aquatic ecosystems.
36) Nature:
Nature encompasses the natural world and its ecosystems, including the interactions between living organisms and their environment. This study's findings contribute to the understanding of how anthropogenic activities like pesticide use disrupt natural processes and impact local biodiversity.
37) Animal:
Animal refers to the living organisms belonging to the kingdom Animalia. In this study, the focus is particularly on fish as key indicators of ecosystem health, exemplifying how environmental toxins can impact animal physiology and reproductive biology.
38) Saxena (Sakshena, Saksena):
Saxena is a cited author in the study, contributing prior knowledge on the effects that pollutants and xenobiotics have on aquatic life. The reference to previous research underlines the study’s foundation in established scientific literature, emphasizing collaborative knowledge development.
39) Filling (Filled):
Filled refers to the content or structures within a biological organ, specifically in this study concerning the ovarian follicles that are filled with developing oocytes. This term emphasizes the normal state of the ovarian structure and how it may be altered by pesticide exposure.
40) Rice (Rce):
Rice is the primary agricultural crop in many regions of India, and butachlor is extensively used for its cultivation. This directly connects the study to agricultural practices, highlighting the interplay between pesticide use in rice fields and the health of aquatic ecosystems.
41) Pose:
Pose refers to the indication or representation of a challenge or threat. In the study, the use of pesticides like butachlor poses significant risks to fish populations and signifies the urgent need for understanding the ecological consequences of such agricultural chemicals on nature.
42) Hand:
Hand metaphorically relates to the extent of human impact on the environment. In this study, the reference to human practices, specifically pesticide applications in agriculture, illustrates how human activities directly influence aquatic ecosystems, reinforcing the responsibility to mitigate such impacts.
Other Science Concepts:
Discover the significance of concepts within the article: �Butachlor causes oxidative, hormonal imbalances in Clarias batrachus.�. Further sources in the context of Science might help you critically compare this page with similair documents:
Financial support, Statistical analysis, Embryonic development, Experimental design, Reproductive cycle, Hormonal imbalance, Oxidative stress, Lipid peroxidation, Reduced glutathione, Thyroid stimulating hormone, Histopathological study, Test group, Environmental Contamination, Biochemical analysis, Scanning Electron Microscopy, Lipid peroxide, Scanning electron microscope, Histopathology, Serum TSH, Standard deviation, Estrogen like effects, Hypothalamo hypophyseal gonadal axis, Glutathione, Free Radical, Vitamin E, Estradiol level, Reduced glutathione (GSH), Thyroid stimulating hormone (TSH), Electron microscopy, Correlative Study, Oxidative stress response, Dehydroascorbic acid, Biochemical observations, Environmental contaminant, Thyroid tissue, Temporal trends.
Concepts being referred in other categories, contexts and sources.