Inhibition of in vitro lipid peroxidation by Calocybe indica.
Journal name: Ancient Science of Life
Original article title: Inhibition of in vitro lipid peroxidation (lpo) evoked by calocybe indica (milky mushroom)
The ANSCI is a peer-reviewed, open-access journal focused on Ayurveda and traditional medicines. It publishes original research, reviews, and literary studies linking traditional knowledge with modern science, covering disciplines like botany, ethnomedicine, pharmacology, and clinical research.
This page presents a generated summary with additional references; See source (below) for actual content.
Original source:
This page is merely a summary which is automatically generated hence you should visit the source to read the original article which includes the author, publication date, notes and references.
S Selvi, P. Umadevi, S. Suja, K. Sridhar, Dr. P. Chinnaswamy
Ancient Science of Life:
(A quarterly multi-disciplinary scientific research journal in Ayurveda)
Full text available for: Inhibition of in vitro lipid peroxidation (lpo) evoked by calocybe indica (milky mushroom)
Year: 2006
Copyright (license): CC BY-NC-SA
Download the PDF file of the original publication
Summary of article contents:
Introduction
The research conducted by Selvi et al. aimed to evaluate the inhibition of lipid peroxidation (LPO) by the aqueous extract of Calocybe indica, also known as milky mushroom, utilizing an in vitro model of goat liver homogenate and red blood cell (RBC) ghosts. LPO represents a significant reaction associated with cellular damage induced by oxygen free radicals, which can contribute to a variety of clinical disorders through an increase in oxidative stress. The antioxidant properties of compounds, including those derived from fungi, have garnered attention in the quest to mitigate cellular damage and enhance health.
The Antioxidant Potential of Calocybe indica
The study demonstrated that the aqueous extract of Calocybe indica effectively inhibited lipid peroxidation in both the goat liver homogenate and RBC ghost models, with a more pronounced effect observed in the RBC membrane model. Specifically, the extract achieved a 71.28% inhibition in RBC ghosts compared to 58.85% in the liver homogenate. These findings suggest that milky mushroom possesses potent antioxidant properties that could potentially protect cellular membranes from oxidative damage. The different extents of inhibition observed between the two models highlight the importance of lipid composition in determining the efficacy of antioxidant agents.
Conclusion
In conclusion, the aqueous extract of Calocybe indica exhibits significant antioxidant activity, as evidenced by its capability to inhibit lipid peroxidation in vitro. This research underscores the potential of edible mushrooms as a valuable source of natural antioxidants, which could play a vital role in the prevention of oxidative stress-related disorders. Further studies are warranted to explore the mechanisms of action and the therapeutic applications of Calocybe indica in clinical settings.
FAQ section (important questions/answers):
What was the objective of the study on Calocybe indica?
The study aimed to assess the inhibition of lipid peroxidation (LPO) by the aqueous extract of Calocybe indica (milky mushroom) using in vitro models of goat liver homogenate and RBC ghosts.
What methods were used for preparing the goat liver homogenate?
Fresh goat liver was obtained, washed, and homogenized in ice-cold Tris Buffered Saline (TBS) to create a 5% homogenate, which was then used for the assay.
How was the aqueous extract of the mushroom prepared?
Air-dried Calocybe indica was powdered and extracted with distilled water. The extract was concentrated, evaporated to dryness, and then resuspended in distilled water for the study.
What were the results of the lipid peroxidation inhibition?
The aqueous extract of Calocybe indica showed a 71.28% inhibition in the RBC ghost model and a 58.85% inhibition in the goat liver homogenate model, indicating stronger effects on the RBC membranes.
Glossary definitions and references:
Scientific and Ayurvedic Glossary list for 鈥淚nhibition of in vitro lipid peroxidation by Calocybe indica.鈥�. This list explains important keywords that occur in this article and links it to the glossary for a better understanding of that concept in the context of Ayurveda and other topics.
1) Medicine:
Medicine refers to the science and practice of diagnosing, treating, and preventing diseases. In the context of the study, it highlights the role of mushrooms like Calocybe indica in providing medicinal benefits such as antioxidant properties which can combat oxidative stress in various clinical disorders.
2) Drug:
A drug is a substance used to diagnose, cure, treat, or prevent diseases. The study discusses the potential of Calocybe indica as a natural drug to inhibit lipid peroxidation, demonstrating its value in pharmaceutical applications and its contribution to holistic health through medicinal mushrooms.
3) Blood:
Blood is a vital fluid in the body responsible for transporting nutrients, oxygen, and waste. In this study, blood (specifically goat blood) is utilized to prepare erythrocyte ghosts, which serve as a model to assess the antioxidant activity of the mushroom extract on lipid peroxidation.
4) Water:
Water is a universal solvent essential for life. The study employs distilled water to extract bioactive compounds from Calocybe indica. The aqueous extract is crucial in assessing the mushroom's ability to inhibit lipid peroxidation, demonstrating its significance in food chemistry and natural medicine.
5) Discussion:
Discussion refers to the section of research where results are interpreted and contextualized. In this study, the discussion encompasses the evaluation of the outcomes related to lipid peroxidation inhibition, comparing the efficacy of Calocybe indica's aqueous extract across different biological models.
6) Antibiotic (Antibacterial):
An antibiotic is a type of medication used to treat bacterial infections. While the study focuses on Calocybe indica's antioxidant properties, the mention of antibiotics highlights the broader context of fungi in medicine, showcasing their diverse therapeutic potentials beyond just antibacterial effects.
7) Substance:
A substance is any material with a definite composition. In the study, Calocybe indica is examined as a natural substance with potential health benefits, specifically in inhibiting lipid peroxidation. Understanding its chemical nature helps elucidate its role in combating oxidative stress.
8) Pesticide:
Pesticides are chemicals used to kill or control pests. The study touches on the role of mushrooms in bioremediation, suggesting that Calocybe indica could absorb harmful substances like pesticides, thereby mitigating environmental damage and underscoring fungi鈥檚 ecological importance in health and safety.
9) Science (Scientific):
Science is the systematic study of the structure and behavior of the physical and natural world. This study exemplifies scientific investigation as it employs rigorous methods to explore the antioxidant effects of Calocybe indica, contributing to the body of knowledge in biochemistry and medical research.
10) Brahmi (Br膩hm墨):
Brahmi refers to a traditional medicinal herb known for its cognitive benefits. Although not directly related to the study, its mention underscores the significance of traditional knowledge in understanding the medicinal applications of natural substances like mushrooms, enriching the cultural context of therapeutic practices.
11) Cancer:
Cancer is a disease characterized by uncontrolled cell growth. The study relates to cancer in the sense that oxidative stress and lipid peroxidation can contribute to cancer development. Calocybe indica's potential as an antioxidant positions it as a candidate for cancer prevention strategies.
Other Science Concepts:
Discover the significance of concepts within the article: 鈥�Inhibition of in vitro lipid peroxidation by Calocybe indica.鈥�. Further sources in the context of Science might help you critically compare this page with similair documents:
Lipid peroxidation, Antioxidant, Clinical disorder, Bioremediation, In vitro model, Calocybe indica, Natural antioxidant defense.