Antibacterial effect of Bulbophyllum neilgherrense: an in vitro study
Journal name: Ancient Science of Life
Original article title: Antibacterial effect of bulbophyllum neilgherrense wt. (orchidaceae). an in vitro study
The ANSCI is a peer-reviewed, open-access journal focused on Ayurveda and traditional medicines. It publishes original research, reviews, and literary studies linking traditional knowledge with modern science, covering disciplines like botany, ethnomedicine, pharmacology, and clinical research.
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Original source:
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K. Priya, C. Krishnaveni
Ancient Science of Life:
(A quarterly multi-disciplinary scientific research journal in Ayurveda)
Full text available for: Antibacterial effect of bulbophyllum neilgherrense wt. (orchidaceae). an in vitro study
Year: 2005
Copyright (license): CC BY-NC-SA
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Summary of article contents:
Introduction
The study investigates the antibacterial activity of Bulbophyllum neilgherrense, an orchid species, against five bacterial strains: Escherichia coli, Staphylococcus aureus, Bacillus pumilus, Pseudomonas aeruginosa, and Pseudomonas putida. Given the adverse effects associated with conventional antibiotics, there is an increasing need for natural substitutes. Orchids, with their extensive history of medicinal application dating back over 3000 years, are rich in alkaloids and phytochemicals, which have been noted for their diverse pharmacological properties, including antibacterial activity.
Antibacterial Efficacy of Orchid Extracts
The research employed various solvent extracts鈥攁queous, ethanolic, and chloroform鈥攐f leaf and pseudobulb from Bulbophyllum neilgherrense to assess their antibacterial potential. The disc diffusion method was utilized, revealing that the ethanolic extracts exhibited the most significant inhibitory effects on bacterial growth, particularly against E. coli, S. aureus, and P. aeruginosa when compared to the other extracts. In contrast, the efficacy of the pseudobulb extract was more pronounced against Pseudomonas species. While these extracts were less effective than the antibiotic streptomycin, they still demonstrated noteworthy antibacterial properties, underscoring the potential of orchid extracts as alternative antimicrobial agents.
Conclusion
The findings from the study highlight the promising antimicrobial potential of Bulbophyllum neilgherrense, suggesting the need for further research to isolate and characterize the specific phytochemicals responsible for its antibacterial effects. With the increasing prevalence of antibiotic resistance, it is vital to explore natural alternatives, and orchids represent a valuable resource in this quest. Continued investigation into the clinical applications of these plant extracts could lead to the development of new therapies for bacterial infections.
FAQ section (important questions/answers):
What is the focus of the study on Bulbophyllum neilgherrense?
The study investigates the antibacterial activity of Bulbophyllum neilgherrense against five bacterial species, including Escherichia coli and Staphylococcus aureus, using aqueous, ethanolic, and chloroform extracts from its leaves and pseudobulbs.
Which extraction method showed the highest antibacterial activity?
Ethanolic extracts exhibited the largest antibacterial effect compared to aqueous and chloroform extracts, specifically against Escherichia coli, Staphylococcus aureus, and Pseudomonas aeruginosa.
What method was employed to test antibacterial efficacy?
The disc diffusion method, also known as the Kirby鈥揃auer method, was utilized to evaluate the antibacterial potential of the plant extracts against the test organisms.
What were the outcomes for each bacterial strain tested?
The study found varying zones of inhibition for different strains, with ethanolic extracts showing superior effectiveness against specific bacteria, thus indicating the antibacterial potential of Bulbophyllum neilgherrense.
Glossary definitions and references:
Scientific and Ayurvedic Glossary list for 鈥淎ntibacterial effect of Bulbophyllum neilgherrense: an in vitro study鈥�. This list explains important keywords that occur in this article and links it to the glossary for a better understanding of that concept in the context of Ayurveda and other topics.
1) Antibiotic (Antibacterial):
Anti-bacterial describes agents that counteract bacteria and prevent their growth, similar to antibacterial. This term emphasizes the focus of the study on testing the extracts from bulbhophylum neilgherrense for their potential anti-bacterial effects, leading to the identification of useful natural antibacterial agents in pharmaceuticals.
2) Water:
Water is a solvent that plays a critical role in biological and chemical processes. In this study, distilled water was used to prepare extracts of bulbhophylum neilgherrense, allowing for the dissolution of phytochemicals, which could exert antibacterial activity against the tested bacteria during the in vitro investigations.
3) Phytochemical:
Phytochemicals are bioactive compounds found in plants that may exhibit various biological activities, including antibacterial effects. The study explores the phytochemical content of bulbhophylum neilgherrense, implying that these compounds are responsible for its medicinal properties and potential therapeutic applications in treating bacterial infections.
4) Activity:
Activity in this context refers to the potential effectiveness of the plant extracts in inhibiting bacterial growth. The study assesses the antibacterial activity of bulbhophylum neilgherrense, evaluating how well its extracts can affect different bacterial species, thereby providing insights into its medicinal properties.
5) Krishnaveni (K峁涐梗峁嘺ve峁嚹�, Krishna-veni):
Krishnaveni is the name of one of the researchers involved in the study. Her contributions, along with Priya K., enhance the credibility of the research findings. The recognition of individual researchers highlights their collective effort in exploring the antibacterial properties of bulbhophylum neilgherrense.
6) Discussion:
Discussion refers to the section of the research paper where results are interpreted and contextualized. It is a critical part of the paper where the efficacy of bulbhophylum neilgherrense extracts is analyzed, compared to other studies, and where implications for future research are suggested, influencing the understanding of its antibacterial properties.
7) Medicine:
Medicine pertains to the science and practice of diagnosing and treating illnesses. The study emphasizes the medicinal value of bulbhophylum neilgherrense, advocating for exploring traditional and natural remedies as potential sources for developing new and effective medicinal treatments, particularly against bacterial infections.
8) Science (Scientific):
Science represents the systematic study of the structure and behavior of the physical and natural world through observation and experimentation. The research on bulbhophylum neilgherrense reflects scientific inquiry aimed at understanding its biological properties, contributing to pharmacognosy and the search for natural antibiotics.
9) Botany:
Botany is the branch of biology that studies plants. In this research, the focus is on bulbhophylum neilgherrense as an important plant species. The study addresses its botanical characteristics and highlights its ethnobotanical significance, encouraging further exploration of plant-based resources in combating bacterial infections.
10) Priya (Priy膩):
Priya is the name of one of the authors collaborating on the research. Her role alongside Krishnaveni contributes significantly to the credibility and authority of the study, indicating collaborative effort in investigating the antibacterial properties of bulbhophylum neilgherrense and its ethnobotanical implications.
11) Soil:
Soil refers to the upper layer of earth where plants grow. It is relevant as the source environment for bulbhophylum neilgherrense. The research highlights the importance of plant integrity and habitat in assessing the potential antibacterial properties of plant extracts, linking environmental factors to medicinal plant efficacy.
Other Science Concepts:
Discover the significance of concepts within the article: 鈥�Antibacterial effect of Bulbophyllum neilgherrense: an in vitro study鈥�. Further sources in the context of Science might help you critically compare this page with similair documents:
Medicinal plant, In vitro study, Disc diffusion method, Zone of inhibition, Aqueous extract, Antibacterial effect, Ethanolic extract, Chloroform extract, Phytochemical content, Bulbophyllum neilgherrense, Bacterial strain, Antimicrobial sensitivity testing, Ethnobotanical importance.