Determination of puerarin from pueraria tuberosa dc by hplc
Journal name: Ancient Science of Life
Original article title: Determination of puerarin from pueraria tuberosa dc by hplc
The ANSCI is a peer-reviewed, open-access journal focused on Ayurveda and traditional medicines. It publishes original research, reviews, and literary studies linking traditional knowledge with modern science, covering disciplines like botany, ethnomedicine, pharmacology, and clinical research.
This page presents a generated summary with additional references; See source (below) for actual content.
Original source:
This page is merely a summary which is automatically generated hence you should visit the source to read the original article which includes the author, publication date, notes and references.
Sateesh Kumar Chauhan, Brijpal Singh, Sudhakar Agrawal
Ancient Science of Life:
(A quarterly multi-disciplinary scientific research journal in Ayurveda)
Full text available for: Determination of puerarin from pueraria tuberosa dc by hplc
Year: 2004
Copyright (license): CC BY-NC-SA
Download the PDF file of the original publication
Summary of article contents:
Introduction
Pueraria tuberosa DC, a member of the Leguminosae family, is renowned in traditional medicine for its diverse therapeutic properties, including its use as an aphrodisiac, tonic, diuretic, and galactogogue. Despite its significant medicinal applications, there has been a lack of standardized analytical methods for quantifying the active compound, puerarin, in this plant. The current study aimed to develop a reliable reverse phase High Pressure Liquid Chromatography (HPLC) method that allows for the determination of puerarin content in P. tuberosa, thereby aiding in its standardization and quality assurance.
Method Development for Puerarin Quantification
The researchers established an HPLC method utilizing a mobile phase composed of acetonitrile, water, and orthophosphoric acid in a 32:67:01 ratio. This setup facilitated effective separation and resolution of puerarin, with optimal detection at 280 nm using a photodiode array detector. A significant finding was the linearity of the calibration graph for puerarin concentrations ranging from 1.0 mg to 8.0 mg, exhibiting a correlation coefficient of 0.9999, indicative of high reliability. The procedure demonstrated an average puerarin content of 3.02% in crude P. tuberosa, with recovery studies showing a percentage recovery of 99.83 ± 0.24%, underscoring the method's accuracy and reproducibility.
Conclusion
The HPLC method developed in this study provides a swift, precise, and effective means of quantifying puerarin in P. tuberosa. This advancement holds importance for the standardization and quality control of herbal medicines that utilize this plant, ensuring that the therapeutic benefits attributed to puerarin can be consistently validated. Ultimately, this method serves not only to enhance the quality assurance of P. tuberosa but also contributes to the broader field of herbal medicine by establishing a foundation for analytical standardization of its active compounds.
FAQ section (important questions/answers):
What is the objective of the HPLC method described in the study?
The study aims to develop a reverse phase HPLC method for determining puerarin in Pueraria tuberosa, which is important for the standardization and quality assessment of this medicinal plant.
What are the main findings regarding puerarin content in P. tuberosa?
The study found an average puerarin content of 3.02% w/w in crude Pueraria tuberosa, indicating its potential as an effective natural source of this active compound.
How was the plant material prepared for HPLC analysis?
Fresh tubers of Pueraria tuberosa were dried, powdered, and extracted with aqueous methanol. The resulting solution was filtered and used for HPLC analysis to determine the puerarin content.
What were the results of the method validation and recovery studies?
The method validation showed a recovery rate of 99.83 ±0.24%, indicating reliability and accuracy. The correlation coefficient of 0.9999 demonstrated excellent linearity for quantifying puerarin in P. tuberosa.
Glossary definitions and references:
Scientific and Ayurvedic Glossary list for “Determination of puerarin from pueraria tuberosa dc by hplc�. This list explains important keywords that occur in this article and links it to the glossary for a better understanding of that concept in the context of Ayurveda and other topics.
1) Water:
Water is a crucial component in the extraction process of active compounds such as puerarin from Pueraria tuberosa. It serves as a solvent in the methanol solution used during extraction. Its role emphasizes the significance of solvent choice in herbal medicine methodologies, impacting the yield and purity of the desired compounds.
2) Medicine:
Medicine refers to the application of scientific methods to study and utilize medicinal plants for therapeutic purposes. In this context, Pueraria tuberosa is used for various health-related benefits. The development of reliable HPLC methods supports the standardization of herbal medicines, ensuring efficacy and safety in their medicinal applications.
3) Discussion:
Discussion in scientific studies encompasses the interpretation of experimental results, comparison with existing literature, and implications for future research. In this study, discussions around the methodology and findings establish a foundation for validating the HPLC technique, emphasizing the importance of analytical methods in herbal medicine research and standardization.
4) Quality:
Quality pertains to the assessment of the purity and concentration of active compounds within herbal products. The study's focus on quantifying puerarin through HPLC showcases quality control measures necessary for herbal medicines. Ensuring high quality via precise analytical techniques is vital for consumer safety and effective therapeutic outcomes.
5) Science (Scientific):
Science serves as the backbone of the methodology utilized in the study. It involves systematic experimentation, measurement, and analysis to explore the properties of Pueraria tuberosa. The application of HPLC represents the intersection of traditional herbal knowledge and modern scientific practices, aiming to enhance the reliability of herbal medicine.
Other Science Concepts:
Discover the significance of concepts within the article: �Determination of puerarin from pueraria tuberosa dc by hplc�. Further sources in the context of Science might help you critically compare this page with similair documents:
HPLC analysis, Chemical analysis, Mobile phase, Ayurvedic Pharmacopoea of India, Phytochemical content, Percentage recovery, Sensitivity of the method, High-pressure liquid chromatography, Linear regression equation, Standard preparation.