TLC and Spectrophotometric Analysis of Embelin in Embelia ribes
Journal name: Ancient Science of Life
Original article title: A TLC Identification and Spectrophotometric Estimation of Embelin in Embelia ribes
The ANSCI is a peer-reviewed, open-access journal focused on Ayurveda and traditional medicines. It publishes original research, reviews, and literary studies linking traditional knowledge with modern science, covering disciplines like botany, ethnomedicine, pharmacology, and clinical research.
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Original source:
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S. K. Chauhan, B. P. Singh, S. Agarwal
Ancient Science of Life:
(A quarterly multi-disciplinary scientific research journal in Ayurveda)
Full text available for: A TLC Identification and Spectrophotometric Estimation of Embelin in Embelia ribes
Year: 1999
Copyright (license): CC BY-NC-SA
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Summary of article contents:
Introduction
Embelia ribes, commonly referred to as "vidang," is a significant medicinal plant in the Ayurvedic system, recognized for its various therapeutic properties, including its use as an anthelmintic and carminative stimulant. The primary active compound within this plant is Embelin, making its quantitative estimation vital for standardizing the therapeutic application of E. ribes. Traditional methods for determining Embelin content have often proven to be time-consuming and lack precision. This study presents a method utilizing Thin Layer Chromatography (TLC) and UV spectrophotometry to provide a rapid, sensitive, and reproducible means of quantifying Embelin in E. ribes.
Quantitative Estimation of Embelin
A central focus of this study is the development of a method for the quantitative estimation of Embelin using UV spectrophotometry. A calibration curve was established using varied concentrations of Embelin, with the absorbance measured at a wavelength of 285 nm. The study found a correlation coefficient of 0.999, indicating a strong relationship between concentration and absorbance. By employing this method, the researchers were able to determine the amount of Embelin present in tested samples of E. ribes, achieving values that indicate both the presence of Embelin and the reliability of the analysis. Recovery studies further validated this method; varying amounts of standard Embelin added to ground E. ribes yielded satisfactory recovery percentages, ensuring that the method can be trusted for accurate quantification.
Conclusion
In conclusion, the developed method for estimating Embelin in Embelia ribes demonstrates a significant advancement in the standardization process for this important medicinal herb. The study highlights the effectiveness of combining TLC for identification and UV spectrophotometry for quantification, overcoming the limitations of previous extraction and estimation techniques. This rapid, simple, and accurate method offers a reliable means to assess Embelin content in E. ribes, supporting its clinical applications and ensuring quality in herbal formulations. The research contributes to the ongoing efforts to establish robust standards in herbal medicine, facilitating better therapeutic outcomes.
FAQ section (important questions/answers):
What method was developed for estimating Embelin in Embelia ribes?
The method developed is based on UV spectrophotometry combined with TLC identification, providing a rapid, precise, and economical means of quantifying Embelin in Embelia ribes.
What is the main active compound in Embelia ribes?
The main active compound in Embelia ribes is Embelin, which is crucial for the standardization of the plant material used in Ayurvedic medicine.
How was the sample prepared for Embelin extraction?
A 1 gm sample of air-dried Embelia ribes was ground and extracted with 15 ml chloroform, repeated multiple times, before final analysis using a UV spectrophotometer.
What were the results of the recovery studies conducted?
The recovery studies showed satisfactory results, with percentages ranging from 98.96% to 100.4%, indicating the reliability of the method for estimating Embelin.
Glossary definitions and references:
Scientific and Ayurvedic Glossary list for “TLC and Spectrophotometric Analysis of Embelin in Embelia ribes�. This list explains important keywords that occur in this article and links it to the glossary for a better understanding of that concept in the context of Ayurveda and other topics.
1) Embelia ribes:
Embelia ribes, commonly known as ‘vidang�, is a medicinal plant from the Myrsinaceae family. It is notable for its anthelmintic, carminative, and stimulant properties, alongside being a vital component in Ayurvedic medicine. The presence of Embelin as a potent bioactive compound makes its quantification in E. ribes essential for standardization and therapeutic use.
2) Drug:
In the context of the text, a drug refers to any substance that is used for medicinal purposes. Here, E. ribes serves as a drug in Ayurveda, highlighting its application in treating ailments. The necessity for standardization and precise compound identification emphasizes the role of various herbal compounds as pharmacologically active constituents.
3) Water:
Water plays a critical role as a solvent and medium in the extraction process of Embelin from E. ribes. In the method described, steam boiling over water is employed to facilitate the extraction of compounds, demonstrating its importance in the preparation and analysis of herbal drugs and their active ingredients.
4) Purification:
Purification in this context refers to steps taken to isolate and identify Embelin from E. ribes. Various methods mentioned indicate the necessity for removing impurities and achieving concentrated samples for accurate measurement. This process underscores the importance of obtaining pure compounds for reliable pharmacological studies and quality assurance in herbal medicine.
5) Discussion:
The section titled Discussion highlights the findings from the study and interprets the significance of results pertaining to the methods used for Embelin quantification. Discussion encompasses the implications of recovery studies and the reliability of the technique, reinforcing the credibility of the proposed method for standardization and quality control of herbal products.
6) Medicine:
Medicine encompasses the various practices, procedures, and substances employed to treat ailments and promote health. In this text, the focus is on Ayurvedic medicine, where E. ribes is utilized for its therapeutic properties. The estimation of Embelin reinforces the connection between herbal compounds and their medicinal applications in healthcare.
7) Science (Scientific):
Science in this context refers to the systematic study and application of methodologies to investigate natural compounds like Embelin. The research represents a structured approach to developing reliable testing methods, emphasizing the interplay between traditional medicine and modern scientific techniques, thereby bridging herbal remedies with validated pharmacological practices.
Other Science Concepts:
Discover the significance of concepts within the article: �TLC and Spectrophotometric Analysis of Embelin in Embelia ribes�. Further sources in the context of Science might help you critically compare this page with similair documents:
Ayurvedic system of medicine, Quantitative estimation, Sample preparation, Calibration curve, Ancient Science of life, Percentage recovery, Method validation, Spectrophotometric estimation, Chromatogram development, Active compound, Calorimetric method, UV-visible recording spectrophotometer, Reproducible method, Standard preparation, Test sample, Gravimetric method.