Significance of Agar well diffusion assay
The Agar well diffusion assay is a laboratory method utilized to evaluate the antibacterial activity of various substances. This technique involves measuring the zone of inhibition created around wells containing test samples in an agar medium, indicating effectiveness against bacteria. It is essential for assessing antimicrobial properties, including those of plant extracts and bacteriocin-producing lactobacilli. The method provides a quantitative analysis of how effectively a substance inhibits bacterial growth by observing and measuring the inhibition zones on agar plates.
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The concept of Agar well diffusion assay in scientific sources
The Agar well diffusion assay is a technique to evaluate the antibacterial activity of extracts by measuring the zones of inhibition on agar plates, thus determining their effectiveness against bacterial growth.
From: The Malaysian Journal of Medical Sciences
(1) A method used to evaluate the antimicrobial activity of substances by observing their effects on indicator strains in agar medium.[1]