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Significance of Agar disc diffusion method

The Agar disc diffusion method is a laboratory technique employed to evaluate the antibacterial activity of various substances by measuring the zone of inhibition on an agar plate. This method involves placing discs impregnated with the test substances on agar plates inoculated with bacteria, where the effectiveness is determined by the size of the inhibition zone that surrounds the discs. It serves as a standardized approach to assess the antimicrobial properties of different extracts, including those from plants.

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The concept of Agar disc diffusion method in scientific sources

Science Books

The Agar disc diffusion method is a laboratory technique employed to evaluate the antimicrobial activity of extracts, specifically measuring the inhibitory effect of Cassia fistula extracts on selected microorganisms through the zone of inhibition.

Significance in Scientific journals, articles, etc.:

From: World Journal of Pharmaceutical Research

(1) A technique to determine the antimicrobial effect of substances by placing discs soaked in the extracts on an agar plate with bacteria.[1] (2) A laboratory technique used to assess the antibacterial activity of plant extracts by measuring the zone of inhibition on an agar plate.[2] (3) A laboratory technique used to evaluate the efficacy of antimicrobial substances by measuring the zone of inhibition around a disc impregnated with the test substance.[3] (4) A method to evaluate the antibacterial activity by measuring the inhibition of bacterial growth around antibiotic-impregnated paper discs placed on agar plates.[4] (5) A laboratory technique used to evaluate the antimicrobial activity of a substance in which the test substance is placed on agar plates inoculated with bacteria; zones of inhibition are measured to assess effectiveness.[5]

From: AYU (Journal of Research in Ayurveda)

(1) A laboratory technique used in this study to assess the antimicrobial activity of the extracts against selected microorganisms.[6] (2) A laboratory technique used in this study to determine the antimicrobial effectiveness of Cassia fistula extracts by measuring the zone of inhibition around discs infused with the extracts.[7]

From: Ancient Science of Life

(1) A method used to determine the antibacterial activity of various extracts of Leptadenia reticulata.[8]

This section includes scientific research in the field of healthcare, often involving traditional medicine systems such as Ayurveda, Unani, Siddha and Holistic health. It includes papers from scientific journals classified as either original, clinical or experimental research, review articles or case reports.