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Phytochemical and antibacterial studies of Limonia acidissima L.

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Journal name: Ancient Science of Life
Original article title: PRELIMINARY STUDIES ON PHYTOCHEMICAL AND ANTIBACTERIAL ACTIVITY OF Limonia acidissima L. PLANT PARTS
The ANSCI is a peer-reviewed, open-access journal focused on Ayurveda and traditional medicines. It publishes original research, reviews, and literary studies linking traditional knowledge with modern science, covering disciplines like botany, ethnomedicine, pharmacology, and clinical research.
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Original source:

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Author:

Asha Thomas, N. R. Ponnammal


Ancient Science of Life:

(A quarterly multi-disciplinary scientific research journal in Ayurveda)

Full text available for: PRELIMINARY STUDIES ON PHYTOCHEMICAL AND ANTIBACTERIAL ACTIVITY OF Limonia acidissima L. PLANT PARTS

Year: 2005

Copyright (license): CC BY-NC-SA


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Summary of article contents:

Introduction

Limonia acidissima L., commonly known as wood apple, is a significant indigenous tree found in India, recognized for its medicinal properties and nutritional value. As a member of the Rutaceae family, this plant is traditionally valued in various cultures, particularly in India where it is associated with sacred groves and is frequently planted in temples and gardens. The study aims to evaluate the phytochemical constituents and antibacterial efficacy of different parts of the Limonia acidissima plant, namely the bark, leaf, rind, pulp, and seed.

Phytochemical and Nutritional Analysis

The analysis revealed a rich composition of phytochemicals across the different plant parts, with the pulp showing a high protein content, while seeds contained a significant amount of carbohydrates. The study further identified a variety of secondary metabolites such as alkaloids, flavonoids, saponins, phenols, and tannins, with varying concentrations depending on the plant part. Notably, the bark and rind had a higher presence of alkaloids, while flavonoids were primarily found in the pulp and seeds. The antibacterial activity tests demonstrated that methanolic extracts from all parts of L. acidissima displayed significant inhibition against both Escherichia coli and Staphylococcus aureus, particularly the pulp, which had the strongest inhibitory effects.

Conclusion

The findings of this study underline the nutritional and medicinal potential of Limonia acidissima, as it is not only a source of vital nutrients but also possesses antibacterial properties that could be exploited for therapeutic applications. Given its rich biochemical composition and traditional uses, L. acidissima represents a valuable resource, both in addressing food security concerns as a �poor man’s food� and as a potential source for natural pharmaceutical products. Promoting the cultivation of this tree could aid in conservation efforts and enhance its availability for medicinal use.

FAQ section (important questions/answers):

What are the main phytochemical constituents of Limonia acidissima?

The main phytochemical constituents include alkaloids, flavonoids, steroids, saponins, glycosides, phenols, gum and mucilage, fixed oils and fats, resins, and tannins, with varying presence in different plant parts.

Which parts of the Limonia acidissima have the highest protein content?

The pulp of Limonia acidissima has the highest protein content at 19.33 mg/g, followed by seeds and leaves, making it a nutritious choice.

How effective are Limonia acidissima extracts against bacteria?

The extracts showed significant antibacterial activity, especially from the pulp, against Escherichia coli and Staphylococcus aureus, indicating its potential use in natural antimicrobial products.

What traditional uses are associated with Limonia acidissima?

Traditionally, Limonia acidissima is valued for its medicinal properties, including use as a stomachic, diuretic, and carminative, addressing digestive issues and often used in temple plantings.

Glossary definitions and references:

Scientific and Ayurvedic Glossary list for “Phytochemical and antibacterial studies of Limonia acidissima L.�. This list explains important keywords that occur in this article and links it to the glossary for a better understanding of that concept in the context of Ayurveda and other topics.

1) Antibiotic (Antibacterial):
Antibiotics are substances that inhibit bacterial growth or kill bacteria. With increasing antibiotic resistance, the search for natural alternatives is vital. The antibacterial properties of Limonia acidissima underscore its potential as a source for novel antibiotic compounds, emphasizing the need for continued exploration of plant-derived antibiotics in contemporary medicine.

2) Phytochemical:
Phytochemicals are bioactive compounds found in plants that contribute to their medicinal properties. In the context of Limonia acidissima, a variety of phytochemicals such as alkaloids, flavonoids, and saponins were identified. These compounds play vital roles in promoting health and preventing diseases, underpinning the importance of phytochemical research in drug development and natural remedies.

3) Activity:
The term 'activity' in this study refers to the measurable effects of the methanolic extracts from Limonia acidissima on bacterial growth. The significance lies in evaluating both biochemical properties and antimicrobial activity, which can indicate the therapeutic potential of the extracts, offering insight into their efficacy as natural antibacterial agents.

4) Limonia acidissima:
Limonia acidissima, commonly known as wood apple, is a medicinal plant belonging to the Rutaceae family. Its parts are traditionally used for various health benefits, such as digestive support and as an antidote for snakebites. This study emphasizes the plant's phytochemical composition and antibacterial properties, validating its use in folk medicine.

5) Flavonoid:
Flavonoids are a class of phytochemicals known for their antioxidant and anti-inflammatory properties. In the study, flavonoids were identified in Limonia acidissima, particularly in the pulp and seeds. Their presence contributes to the plant's medicinal potential, highlighting interest in flavonoid-rich sources for developing new health supplements and therapeutic products.

6) Science (Scientific):
Science reflects the systematic study of the natural world through observation and experimentation. The research presented on Limonia acidissima employs scientific methods in phytochemical analysis and antibacterial testing. This approach underscores the role of scientific inquiry in validating traditional knowledge and enhancing our understanding of plant-based medicines.

7) Botany:
Botany is the scientific study of plants, encompassing their structure, growth, and medicinal uses. The research conducted on Limonia acidissima illustrates key botanical concepts by analyzing plant parts for phytochemical content and medicinal applications. It highlights botany’s role in exploring the vast reservoir of plant resources for potential therapeutic uses.

8) Aegle marmelos:
Aegle marmelos, also known as bael, is another plant studied for its medicinal properties. Cited in relation to Limonia acidissima, findings on Aegle marmelos suggest similar phytochemical profiles and health benefits. Comparisons between these two plants bolster the exploration of medicinal plants and promote the potential for shared therapeutic insights.

9) Discussion:
The discussion section of the study reflects on the significance of findings related to the biochemical composition and antibacterial activity of Limonia acidissima. It offers insights into the implications of the research, linking traditional uses of the plant with modern applications in medicine. Critical examination helps contextualize results within existing scientific literature.

10) Medicine:
Medicine encompasses the science and practice of diagnosing, treating, and preventing disease. The study of Limonia acidissima highlights its historical and cultural significance in traditional medicine, alongside its potential for modern therapeutic application. This intersection of traditional and contemporary medicine signifies the importance of herbal remedies in healthcare.

11) Reason:
Reason pertains to the rationale behind scientific investigation. In this study, the researchers sought to understand the phytochemical composition and antibacterial activity of Limonia acidissima to validate its traditional uses in medicine. Establishing scientific evidence provides a foundation for further research and applications of plant-based therapies.

12) Water:
Water is a critical solvent in biological systems and is essential for the extraction processes in phytochemical studies. The research utilized methanol for extraction; however, the role of water is paramount in plant biology, affecting growth, biochemical processes, and the efficacy of medicinal compounds extracted from plants like Limonia acidissima.

13) Drug:
Drugs are substances used for medical treatment, and in the context of this study, the exploration of Limonia acidissima's antibacterial properties supports its potential as a natural drug candidate. Investigating plant extracts for their inhibitory effects on bacteria plays a crucial role in discovering new drug formulations that may be safer and more accessible.

Other Science Concepts:

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Discover the significance of concepts within the article: �Phytochemical and antibacterial studies of Limonia acidissima L.�. Further sources in the context of Science might help you critically compare this page with similair documents:

Limonia acidissima, Sacred grove, Traditional societies, Antibacterial activity, Disc diffusion method, Biochemical Composition, Biochemical analysis, Inhibition zone, Methanolic extract, Carbohydrate content, Preliminary phytochemical analysis, Plant part, Gram-negative and gram-positive bacteria, Antidote to snakebite, Poor man's food.

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