Extraction and study of diastase-like enzyme from piper betle.
Journal name: Ancient Science of Life
Original article title: Extraction and physico-chemical studies of diastase-like enzyme from piper betle petioles: part 1
The ANSCI is a peer-reviewed, open-access journal focused on Ayurveda and traditional medicines. It publishes original research, reviews, and literary studies linking traditional knowledge with modern science, covering disciplines like botany, ethnomedicine, pharmacology, and clinical research.
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Original source:
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G.V.S Ramasarma, Sadhan Kumar Dutta
Ancient Science of Life:
(A quarterly multi-disciplinary scientific research journal in Ayurveda)
Full text available for: Extraction and physico-chemical studies of diastase-like enzyme from piper betle petioles: part 1
Year: 1995
Copyright (license): CC BY-NC-SA
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Summary of article contents:
Introduction
The study investigates the extraction and investigation of a diastase-like enzyme from the petioles of the Piper betle plant, particularly the Bengal variety. This research stems from a growing interest in plant products for their biological and pharmacological properties, with previous studies highlighting the medicinal benefits of Piper betle leaves for various health conditions, including respiratory infections and external wound healing. The focus of this study is to evaluate the crude extract of petioles for its carbohydrate hydrolytic activity, contributing to our understanding of its potential therapeutic applications.
Diastase-Like Activity of Piper Betle Petioles
One of the key findings of the study is the presence of amylase-like activity in the crude extract from the petioles of Piper betle. The analysis revealed an amylase activity of 64 units per milligram when the extract was tested under various temperature and pH conditions. The extract exhibited a significant reduction in the viscosity of starch solutions, indicating its enzymatic action. The optimal conditions for this activity were found to be at a temperature of 35°C and a pH range of 5.6 to 7.9, demonstrating that both temperature and pH significantly influence the enzyme's performance. This discovery underscores the potential of Piper betle as a source of useful biological enzymes for applications in medicine and industry.
Conclusion
The preliminary findings from the extraction and analysis of the petiolous extract of Piper betle suggest that it possesses valuable carbohydrate hydrolytic properties. The observed diastase-like activity opens avenues for further investigations into the purification and potential pharmacological uses of this extract. Future research aims to clarify the biochemical properties and therapeutic potentials of the extracted enzyme, which could lead to innovative applications in both health and medicinal fields. The study lays the groundwork for further exploration of Piper betle's contributions to science and medicine, affirming the importance of traditional plant resources in modern therapeutics.
FAQ section (important questions/answers):
What was the focus of the study on Piper betle petioles?
The study aimed to extract and evaluate the diastase-like enzyme from Piper betle petioles, along with assessing its physicochemical properties to explore potential biological and pharmacological activities.
What methods were used for enzyme activity detection?
Enzyme activity was assessed using a standard procedure with a phosphate buffer. It involved monitoring the reaction with starch solution and titrating the products formed against sodium thiosulfate.
What were the optimal conditions for enzyme activity observed?
The study found that the optimal temperature for enzymatic activity was 35°C. The ideal pH levels were determined to be around 5.6 to 5.8 for citrate buffer and 7.9 for phosphate buffer.
What were the key findings regarding viscosity reduction?
The crude extract from Piper betle significantly reduced the viscosity of starch solution at all investigated temperatures, indicating substantial amylase-like activity present in the extract.
Glossary definitions and references:
Scientific and Ayurvedic Glossary list for “Extraction and study of diastase-like enzyme from piper betle.�. This list explains important keywords that occur in this article and links it to the glossary for a better understanding of that concept in the context of Ayurveda and other topics.
1) Activity:
In the context of this study, 'activity' refers to the functional capacity of the crude extract obtained from Piper betle petioles, particularly its ability to exhibit diastase-like enzyme action. Measuring the enzymatic activity helps in evaluating potential therapeutic benefits and further understanding the biological role of the extract.
2) Pharmacological:
The term 'pharmacological' pertains to the study of how natural compounds, like those extracted from Piper betle, can impact biological systems. This research aims to investigate the potential medicinal properties, safety, and effectiveness of plant-derived substances, contributing to the development of new therapeutic agents in modern medicine.
3) Water:
Water serves as a crucial medium in the extraction process of the diastase-like enzyme. In this study, phosphate buffer and distilled water are utilized to maintain pH levels and solubilize compounds. Understanding how water interacts with the plant extracts is vital for assessing their enzymatic activity and stability.
4) Purification:
Purification refers to the process of isolating the diastase-like enzyme from the crude extract. This step is essential to identify the specific properties and effective components of the extract, enabling researchers to study the enzyme's biochemical and pharmacological characteristics in more detail and assess its potential applications.
5) Discussion:
The 'discussion' section is where the findings of this study are analyzed, interpreted, and compared with existing literature. Important implications, limitations, and future research directions regarding the diastase-like enzyme's activity and its pharmacological potential are presented, allowing for a deeper understanding of the significance of the results.
6) Science (Scientific):
Science is the overarching discipline that encompasses the methodology and systematic study of natural phenomena. In this research, scientific principles are applied to explore the biochemical properties of Piper betle and its extract, aiming to demonstrate how plant-based substances can offer biological and pharmacological significance.
7) Substance:
In this context, 'substance' refers to the distinct compounds or extracts derived from plant materials. The study investigates the physical and chemical properties of the crude extract obtained from Piper betle petioles, focusing on its enzyme activity. Understanding these substances can lead to insights into their potential health benefits.
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