Significance of HPTLC method
High-Performance Thin Layer Chromatography (HPTLC) is a versatile analytical technique utilized for the separation, identification, and quantification of compounds in various samples. The method is widely applied in pharmaceutical research, herbal drug analysis, and chemical assessments of plant extracts. HPTLC offers advantages like sensitivity, simplicity, and specificity, making it suitable for analyzing both qualitative and quantitative aspects of substances. Its validation according to ICH guidelines enhances its reliability in determining the composition of complex mixtures and formulations.
Synonyms: High-performance thin-layer chromatography (hptlc), Chromatographic technique, Separation technique, High-performance thin-layer chromatography method, Tlc analysis
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The concept of HPTLC method in scientific sources
The HPTLC method is a validated chromatographic technique essential for quantifying marker compounds, evaluating almond oil's constituents, and determining Piperine levels in Ayurvedic formulations, adhering to ICH guidelines for accuracy and precision.
From: World Journal of Pharmaceutical Research
(1) High-Performance Thin Layer Chromatography, a technique used for separating and analyzing compounds in mixtures.[1] (2) High Performance Thin Layer Chromatography method used for the quantitative analysis and separation of chemical substances in pharmaceutical formulations.[2] (3) High-Performance Thin Layer Chromatography (HPTLC) is a technique used for separating and analyzing substances in a mixture.[3] (4) High-Performance Thin Layer Chromatography method used for the separation and analysis of substances.[4] (5) High-Performance Thin-Layer Chromatography, a technique used for quantitative analysis of samples.[5]
From: International Ayurvedic Medical Journal
(1) A chromatographic technique used for the quantification of marker compounds in formulations, developed and validated for accuracy and precision as per ICH guidelines.[6]
From: Journal of Ayurveda and Integrative Medicine
(1) High-Performance Thin-Layer Chromatography technique used for the detection and quantification of chemical substances, specifically piperine in this context.[7]