Significance of Enzyme-linked immunosorbent assay
Enzyme-linked immunosorbent assay (ELISA) is a versatile laboratory technique used to detect and quantify various proteins, antibodies, and antigens in samples. It is commonly employed in measuring concentrations of cytokines, such as TNF-α; detecting hepatitis B surface antigens; and analyzing biomarkers related to bone health and diseases, including cancer. Additionally, ELISA can evaluate specific proteins like Interferon gamma and adiponectin, making it an essential tool for diagnostics across numerous biological samples and clinical conditions.
Synonyms: Elisa, Enzyme immunoassay, Immunoassay, Enzyme assay, Antibody test
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The concept of Enzyme-linked immunosorbent assay in scientific sources
Enzyme-linked immunosorbent assay (ELISA) is a biochemical technique essential for diagnosing diseases, notably by measuring CA15-3 levels for breast and ovarian cancer and assessing hormone concentrations, such as testosterone, in various biological samples.
From: The Malaysian Journal of Medical Sciences
(1) The method used to measure fasting plasma insulin, helping to assess insulin sensitivity in the rats.[1] (2) This is a test used to evaluate the level of anti-H. pylori immunoglobulin G in this study, which helps to identify the presence of the infection.[2] (3) Enzyme-linked immunosorbent assays is a method used to detect antibodies and the detection of JE virus IgM antibodies was carried out by this method.[3] (4) Enzyme-linked immunosorbent assay is a laboratory technique (ELISA) used to measure the levels of adiponectin and leptin in serum or plasma, providing quantitative data for assessing the impact of exercise and other factors.[4] (5) This immunological technique is discussed in the context of detecting ruminant fascioliasis and summarises the performance of various assays in studies.[5]