Significance of Electrophoresis
Electrophoresis is a versatile laboratory technique used to separate macromolecules such as proteins and DNA based on their size and charge. This method is essential for various applications, including confirming the purity and molecular weight of proteins, analyzing PCR products and RFLP, and visualizing specific genes. Electrophoresis involves using an electric field to facilitate the separation process, commonly employed in mutation analysis and classifying different types of von Willebrand disease. Overall, it is a crucial tool in molecular biology research.
Synonyms: Gel electrophoresis, Paper electrophoresis, Capillary electrophoresis, Isoelectric focusing
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The concept of Electrophoresis in scientific sources
Electrophoresis is a laboratory technique that separates molecules by size and charge, applicable in detecting enzymatic activity, separating DNA fragments, and facilitating the movement of charged drug molecules through the skin using an electric field.
From: The Malaysian Journal of Medical Sciences
(1) A technique used to separate molecules, like DNA fragments, based on size and charge, used to analyze PCR products, and can be done with agarose gels.[1] (2) A laboratory technique used to separate charged particles, commonly proteins or nucleic acids, based on their size and charge.[2] (3) A laboratory technique used to separate DNA fragments based on their size through a gel matrix, commonly used in mutation analysis.[3]